Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in Australia
Antibiotic resistance genes (ARGs) including those from the <i>bla</i><sub>CTX-M</sub> family and <i>mcr-1</i> that encode resistance to extended spectrum β–lactams and colistin, respectively, have been linked with IncHI2 plasmids isolated from swine production fa...
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/896 |
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| author | Tiziana Zingali Toni A. Chapman John Webster Piklu Roy Chowdhury Steven P. Djordjevic |
| author_facet | Tiziana Zingali Toni A. Chapman John Webster Piklu Roy Chowdhury Steven P. Djordjevic |
| author_sort | Tiziana Zingali |
| collection | DOAJ |
| description | Antibiotic resistance genes (ARGs) including those from the <i>bla</i><sub>CTX-M</sub> family and <i>mcr-1</i> that encode resistance to extended spectrum β–lactams and colistin, respectively, have been linked with IncHI2 plasmids isolated from swine production facilities globally but not in IncHI2 plasmids from Australia. Here we describe the first complete sequence of a multiple drug resistance Australian IncHI2-ST4 plasmid, pTZ41_1P, from a commensal <i>E. coli</i> from a healthy piglet. pTZ41_1P carries genes conferring resistance to heavy-metals (copper, silver, tellurium and arsenic), β-lactams, aminoglycosides and sulphonamides. The ARGs reside within a complex resistance locus (CRL) that shows considerable sequence identity to a CRL in pSDE_SvHI2, an IncHI2:ST3 plasmid from an enterotoxigenic <i>E. coli</i> with serotype O157:H19 of porcine origin that caused substantial losses to swine production operations in Australia in 2007. pTZ41_1P is closely related to IncHI2 plasmids found in <i>E. coli</i> and <i>Salmonella enterica</i> from porcine, avian and human sources in Europe and China but it does not carry genes encoding resistance to clinically-important antibiotics. We identified regions of IncHI2 plasmids that contribute to the genetic plasticity of this group of plasmids and highlight how they may readily acquire new resistance gene cargo. Genomic surveillance should be improved to monitor IncHI2 plasmids. |
| first_indexed | 2024-03-10T19:10:56Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:10:56Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-364a99fbfd2e46e1a40ca7137b1571362023-11-20T03:46:51ZengMDPI AGMicroorganisms2076-26072020-06-018689610.3390/microorganisms8060896Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in AustraliaTiziana Zingali0Toni A. Chapman1John Webster2Piklu Roy Chowdhury3Steven P. Djordjevic4The ithree Institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, AustraliaNSW Department of Primary Industries, Elizabeth MacArthur Agricultural Institute, Menangle, NSW 2568, AustraliaNSW Department of Primary Industries, Elizabeth MacArthur Agricultural Institute, Menangle, NSW 2568, AustraliaThe ithree Institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, AustraliaThe ithree Institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, AustraliaAntibiotic resistance genes (ARGs) including those from the <i>bla</i><sub>CTX-M</sub> family and <i>mcr-1</i> that encode resistance to extended spectrum β–lactams and colistin, respectively, have been linked with IncHI2 plasmids isolated from swine production facilities globally but not in IncHI2 plasmids from Australia. Here we describe the first complete sequence of a multiple drug resistance Australian IncHI2-ST4 plasmid, pTZ41_1P, from a commensal <i>E. coli</i> from a healthy piglet. pTZ41_1P carries genes conferring resistance to heavy-metals (copper, silver, tellurium and arsenic), β-lactams, aminoglycosides and sulphonamides. The ARGs reside within a complex resistance locus (CRL) that shows considerable sequence identity to a CRL in pSDE_SvHI2, an IncHI2:ST3 plasmid from an enterotoxigenic <i>E. coli</i> with serotype O157:H19 of porcine origin that caused substantial losses to swine production operations in Australia in 2007. pTZ41_1P is closely related to IncHI2 plasmids found in <i>E. coli</i> and <i>Salmonella enterica</i> from porcine, avian and human sources in Europe and China but it does not carry genes encoding resistance to clinically-important antibiotics. We identified regions of IncHI2 plasmids that contribute to the genetic plasticity of this group of plasmids and highlight how they may readily acquire new resistance gene cargo. Genomic surveillance should be improved to monitor IncHI2 plasmids.https://www.mdpi.com/2076-2607/8/6/896IncHI2plasmidporcine<i>mefB</i>complex resistance locusmultiple drug resistance |
| spellingShingle | Tiziana Zingali Toni A. Chapman John Webster Piklu Roy Chowdhury Steven P. Djordjevic Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in Australia Microorganisms IncHI2 plasmid porcine <i>mefB</i> complex resistance locus multiple drug resistance |
| title | Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in Australia |
| title_full | Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in Australia |
| title_fullStr | Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in Australia |
| title_full_unstemmed | Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in Australia |
| title_short | Genomic Characterisation of a Multiple Drug Resistant IncHI2 ST4 Plasmid in <i>Escherichia coli</i> ST744 in Australia |
| title_sort | genomic characterisation of a multiple drug resistant inchi2 st4 plasmid in i escherichia coli i st744 in australia |
| topic | IncHI2 plasmid porcine <i>mefB</i> complex resistance locus multiple drug resistance |
| url | https://www.mdpi.com/2076-2607/8/6/896 |
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