Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant Bacteria
Antibiotic resistance is one of the most important global problems currently confronting the world. Different biomedical applications of silver nanoparticles (AgNPs) have indicated them to be promising antimicrobial agents. In the present study, extracellular extract of an endophytic bacterium, <...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/23/12/3220 |
| _version_ | 1819160507499675648 |
|---|---|
| author | Tahmina Monowar Md. Sayedur Rahman Subhash J. Bhore Gunasunderi Raju Kathiresan V. Sathasivam |
| author_facet | Tahmina Monowar Md. Sayedur Rahman Subhash J. Bhore Gunasunderi Raju Kathiresan V. Sathasivam |
| author_sort | Tahmina Monowar |
| collection | DOAJ |
| description | Antibiotic resistance is one of the most important global problems currently confronting the world. Different biomedical applications of silver nanoparticles (AgNPs) have indicated them to be promising antimicrobial agents. In the present study, extracellular extract of an endophytic bacterium, <i>Pantoea ananatis,</i> was used for synthesis of AgNPs. The synthesized AgNPs were characterized by UV⁻Vis spectroscopy, FTIR, transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and Zeta potential. The antimicrobial potential of the AgNPs against pathogenic <i>Staphylococcus aureus</i> subsp. <i>aureus</i> (ATCC 11632), <i>Bacillus cereus</i> (ATCC 10876), <i>Escherichia coli</i> (ATCC 10536), <i>Pseudomonas aeruginosa</i> (ATCC 10145) and <i>Candida albicans</i> (ATCC 10231), and multidrug resistant (MDR) <i>Streptococcus pneumoniae</i> (ATCC 700677), <i>Enterococcus faecium</i> (ATCC 700221) <i>Staphylococcus aureus</i> (ATCC 33592) <i>Escherichia coli</i> (NCTC 13351) was investigated. The synthesized spherical-shaped AgNPs with a size range of 8.06 nm to 91.32 nm exhibited significant antimicrobial activity at 6 μg/disc concentration against <i>Bacillus cereus</i> (ATCC 10876) and <i>Candida albicans</i> (ATCC 10231) which were found to be resistant to conventional antibiotics. The synthesized AgNPs showed promising antibacterial efficiency at 10 µg/disc concentration against the MDR strains. The present study suggests that AgNPs synthesized by using the endophytic bacterium <i>P. ananatis</i> are promising antimicrobial agent. |
| first_indexed | 2024-12-22T16:57:33Z |
| format | Article |
| id | doaj.art-364ad5ed4903469cbb7f591fb8959f09 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T16:57:33Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-364ad5ed4903469cbb7f591fb8959f092022-12-21T18:19:25ZengMDPI AGMolecules1420-30492018-12-012312322010.3390/molecules23123220molecules23123220Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant BacteriaTahmina Monowar0Md. Sayedur Rahman1Subhash J. Bhore2Gunasunderi Raju3Kathiresan V. Sathasivam4Unit of Microbiology, Faculty of Medicine, AIMST University, Kedah 08100, MalaysiaRegional Director, Ministry of Information, Government of the People’s Republic of Bangladesh, Gopalganj 8100, BangladeshDepartment of Biotechnology, Faculty of Applied Sciences, AIMST University, Kedah 08100, MalaysiaSchool of Distance Education, Universiti Sains Malaysia, Pulau Pinang 11800, MalaysiaDepartment of Biotechnology, Faculty of Applied Sciences, AIMST University, Kedah 08100, MalaysiaAntibiotic resistance is one of the most important global problems currently confronting the world. Different biomedical applications of silver nanoparticles (AgNPs) have indicated them to be promising antimicrobial agents. In the present study, extracellular extract of an endophytic bacterium, <i>Pantoea ananatis,</i> was used for synthesis of AgNPs. The synthesized AgNPs were characterized by UV⁻Vis spectroscopy, FTIR, transmission electron microscopy (TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX), and Zeta potential. The antimicrobial potential of the AgNPs against pathogenic <i>Staphylococcus aureus</i> subsp. <i>aureus</i> (ATCC 11632), <i>Bacillus cereus</i> (ATCC 10876), <i>Escherichia coli</i> (ATCC 10536), <i>Pseudomonas aeruginosa</i> (ATCC 10145) and <i>Candida albicans</i> (ATCC 10231), and multidrug resistant (MDR) <i>Streptococcus pneumoniae</i> (ATCC 700677), <i>Enterococcus faecium</i> (ATCC 700221) <i>Staphylococcus aureus</i> (ATCC 33592) <i>Escherichia coli</i> (NCTC 13351) was investigated. The synthesized spherical-shaped AgNPs with a size range of 8.06 nm to 91.32 nm exhibited significant antimicrobial activity at 6 μg/disc concentration against <i>Bacillus cereus</i> (ATCC 10876) and <i>Candida albicans</i> (ATCC 10231) which were found to be resistant to conventional antibiotics. The synthesized AgNPs showed promising antibacterial efficiency at 10 µg/disc concentration against the MDR strains. The present study suggests that AgNPs synthesized by using the endophytic bacterium <i>P. ananatis</i> are promising antimicrobial agent.https://www.mdpi.com/1420-3049/23/12/3220endophytic bacterium<i>Pantoea ananatis</i>silver nanoparticlespathogenic microbesmultidrug resistant bacteria |
| spellingShingle | Tahmina Monowar Md. Sayedur Rahman Subhash J. Bhore Gunasunderi Raju Kathiresan V. Sathasivam Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant Bacteria Molecules endophytic bacterium <i>Pantoea ananatis</i> silver nanoparticles pathogenic microbes multidrug resistant bacteria |
| title | Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant Bacteria |
| title_full | Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant Bacteria |
| title_fullStr | Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant Bacteria |
| title_full_unstemmed | Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant Bacteria |
| title_short | Silver Nanoparticles Synthesized by Using the Endophytic Bacterium <i>Pantoea ananatis</i> are Promising Antimicrobial Agents against Multidrug Resistant Bacteria |
| title_sort | silver nanoparticles synthesized by using the endophytic bacterium i pantoea ananatis i are promising antimicrobial agents against multidrug resistant bacteria |
| topic | endophytic bacterium <i>Pantoea ananatis</i> silver nanoparticles pathogenic microbes multidrug resistant bacteria |
| url | https://www.mdpi.com/1420-3049/23/12/3220 |
| work_keys_str_mv | AT tahminamonowar silvernanoparticlessynthesizedbyusingtheendophyticbacteriumipantoeaananatisiarepromisingantimicrobialagentsagainstmultidrugresistantbacteria AT mdsayedurrahman silvernanoparticlessynthesizedbyusingtheendophyticbacteriumipantoeaananatisiarepromisingantimicrobialagentsagainstmultidrugresistantbacteria AT subhashjbhore silvernanoparticlessynthesizedbyusingtheendophyticbacteriumipantoeaananatisiarepromisingantimicrobialagentsagainstmultidrugresistantbacteria AT gunasunderiraju silvernanoparticlessynthesizedbyusingtheendophyticbacteriumipantoeaananatisiarepromisingantimicrobialagentsagainstmultidrugresistantbacteria AT kathiresanvsathasivam silvernanoparticlessynthesizedbyusingtheendophyticbacteriumipantoeaananatisiarepromisingantimicrobialagentsagainstmultidrugresistantbacteria |