Insect endosymbiont proliferation is limited by lipid availability
Spiroplasma poulsonii is a maternally transmitted bacterial endosymbiont that is naturally associated with Drosophila melanogaster. S. poulsonii resides extracellularly in the hemolymph, where it must acquire metabolites to sustain proliferation. In this study, we find that Spiroplasma proliferation...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/02964 |
| _version_ | 1811200340058439680 |
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| author | Jeremy K Herren Juan C Paredes Fanny Schüpfer Karim Arafah Philippe Bulet Bruno Lemaitre |
| author_facet | Jeremy K Herren Juan C Paredes Fanny Schüpfer Karim Arafah Philippe Bulet Bruno Lemaitre |
| author_sort | Jeremy K Herren |
| collection | DOAJ |
| description | Spiroplasma poulsonii is a maternally transmitted bacterial endosymbiont that is naturally associated with Drosophila melanogaster. S. poulsonii resides extracellularly in the hemolymph, where it must acquire metabolites to sustain proliferation. In this study, we find that Spiroplasma proliferation specifically depletes host hemolymph diacylglyceride, the major lipid class transported by the lipoprotein, Lpp. RNAi-mediated knockdown of Lpp expression, which reduces the amount of circulating lipids, inhibits Spiroplasma proliferation demonstrating that bacterial proliferation requires hemolymph-lipids. Altogether, our study shows that an insect endosymbiont acquires specific lipidic metabolites from the transport lipoproteins in the hemolymph of its host. In addition, we show that the proliferation of this endosymbiont is limited by the availability of hemolymph lipids. This feature could limit endosymbiont over-proliferation under conditions of host nutrient limitation as lipid availability is strongly influenced by the nutritional state. |
| first_indexed | 2024-04-12T02:03:14Z |
| format | Article |
| id | doaj.art-364cd8ee56dd47e5bb35793d7fc0a72c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:03:14Z |
| publishDate | 2014-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-364cd8ee56dd47e5bb35793d7fc0a72c2022-12-22T03:52:37ZengeLife Sciences Publications LtdeLife2050-084X2014-07-01310.7554/eLife.02964Insect endosymbiont proliferation is limited by lipid availabilityJeremy K Herren0https://orcid.org/0000-0003-2239-7275Juan C Paredes1Fanny Schüpfer2Karim Arafah3Philippe Bulet4Bruno Lemaitre5Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, SwitzerlandGlobal Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, SwitzerlandGlobal Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, SwitzerlandPlatform BioPark Archamps, Saint Julien en Genevois, FrancePlatform BioPark Archamps, Saint Julien en Genevois, France; Université Joseph Fourier, AGIM FRE CNRS, La Tronche, FranceGlobal Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, SwitzerlandSpiroplasma poulsonii is a maternally transmitted bacterial endosymbiont that is naturally associated with Drosophila melanogaster. S. poulsonii resides extracellularly in the hemolymph, where it must acquire metabolites to sustain proliferation. In this study, we find that Spiroplasma proliferation specifically depletes host hemolymph diacylglyceride, the major lipid class transported by the lipoprotein, Lpp. RNAi-mediated knockdown of Lpp expression, which reduces the amount of circulating lipids, inhibits Spiroplasma proliferation demonstrating that bacterial proliferation requires hemolymph-lipids. Altogether, our study shows that an insect endosymbiont acquires specific lipidic metabolites from the transport lipoproteins in the hemolymph of its host. In addition, we show that the proliferation of this endosymbiont is limited by the availability of hemolymph lipids. This feature could limit endosymbiont over-proliferation under conditions of host nutrient limitation as lipid availability is strongly influenced by the nutritional state.https://elifesciences.org/articles/02964SpiroplasmaSymbiosisEndosymbiosisLipophorindiacylglyceridecardiolipin |
| spellingShingle | Jeremy K Herren Juan C Paredes Fanny Schüpfer Karim Arafah Philippe Bulet Bruno Lemaitre Insect endosymbiont proliferation is limited by lipid availability eLife Spiroplasma Symbiosis Endosymbiosis Lipophorin diacylglyceride cardiolipin |
| title | Insect endosymbiont proliferation is limited by lipid availability |
| title_full | Insect endosymbiont proliferation is limited by lipid availability |
| title_fullStr | Insect endosymbiont proliferation is limited by lipid availability |
| title_full_unstemmed | Insect endosymbiont proliferation is limited by lipid availability |
| title_short | Insect endosymbiont proliferation is limited by lipid availability |
| title_sort | insect endosymbiont proliferation is limited by lipid availability |
| topic | Spiroplasma Symbiosis Endosymbiosis Lipophorin diacylglyceride cardiolipin |
| url | https://elifesciences.org/articles/02964 |
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