Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
Abstract Background Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a 1H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced ex...
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1777-1 |
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| author | Amalina Ahmad Azam Raghunath Pariyani Intan Safinar Ismail Amin Ismail Alfi Khatib Faridah Abas Khozirah Shaari |
| author_facet | Amalina Ahmad Azam Raghunath Pariyani Intan Safinar Ismail Amin Ismail Alfi Khatib Faridah Abas Khozirah Shaari |
| author_sort | Amalina Ahmad Azam |
| collection | DOAJ |
| description | Abstract Background Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a 1H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimental model in rats. Methods Four different solvent extracts of OS, namely aqueous, ethanolic, 50% aqueous ethanolic and methanolic, at a dose of 500 mg/kg body weight (bw) were orally administered for 14 days to diabetic rats induced via intraperitoneal injection of 60 mg/kg bw STZ. NMR metabolomics approach using pattern recognition combined with multivariate statistical analysis was applied in the rat urine to study the resulted metabolic perturbations. Results OS aqueous extract (OSAE) caused a reversal of DM comparable to that of 10 mg/kg bw glibenclamide. A total of 15 urinary metabolites, which levels changed significantly upon treatment were identified as the biomarkers of OSAE in diabetes. A systematic metabolic pathways analysis identified that OSAE contributed to the antidiabetic activity mainly through regulating the tricarboxylic acid cycle, glycolysis/gluconeogenesis, lipid and amino acid metabolism. Conclusions The results of this study validated the ethnopharmacological use of OS in diabetes and unveiled the biochemical and metabolic mechanisms involved. |
| first_indexed | 2024-12-21T04:02:26Z |
| format | Article |
| id | doaj.art-364d84d21b7941d1b2c295f492addd54 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-21T04:02:26Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-364d84d21b7941d1b2c295f492addd542022-12-21T19:16:40ZengBMCBMC Complementary and Alternative Medicine1472-68822017-05-0117111310.1186/s12906-017-1777-1Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopyAmalina Ahmad Azam0Raghunath Pariyani1Intan Safinar Ismail2Amin Ismail3Alfi Khatib4Faridah Abas5Khozirah Shaari6Laboratory of Natural Products, Institute of Bioscience, Universiti Putra MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra MalaysiaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaFaculty of Pharmacy, International Islamic University MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra MalaysiaLaboratory of Natural Products, Institute of Bioscience, Universiti Putra MalaysiaAbstract Background Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a 1H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimental model in rats. Methods Four different solvent extracts of OS, namely aqueous, ethanolic, 50% aqueous ethanolic and methanolic, at a dose of 500 mg/kg body weight (bw) were orally administered for 14 days to diabetic rats induced via intraperitoneal injection of 60 mg/kg bw STZ. NMR metabolomics approach using pattern recognition combined with multivariate statistical analysis was applied in the rat urine to study the resulted metabolic perturbations. Results OS aqueous extract (OSAE) caused a reversal of DM comparable to that of 10 mg/kg bw glibenclamide. A total of 15 urinary metabolites, which levels changed significantly upon treatment were identified as the biomarkers of OSAE in diabetes. A systematic metabolic pathways analysis identified that OSAE contributed to the antidiabetic activity mainly through regulating the tricarboxylic acid cycle, glycolysis/gluconeogenesis, lipid and amino acid metabolism. Conclusions The results of this study validated the ethnopharmacological use of OS in diabetes and unveiled the biochemical and metabolic mechanisms involved.http://link.springer.com/article/10.1186/s12906-017-1777-1Orthosiphon StamineusJava teaMetabolomicsDiabetes mellitus1H NMR spectroscopy |
| spellingShingle | Amalina Ahmad Azam Raghunath Pariyani Intan Safinar Ismail Amin Ismail Alfi Khatib Faridah Abas Khozirah Shaari Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy BMC Complementary and Alternative Medicine Orthosiphon Stamineus Java tea Metabolomics Diabetes mellitus 1H NMR spectroscopy |
| title | Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
| title_full | Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
| title_fullStr | Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
| title_full_unstemmed | Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
| title_short | Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy |
| title_sort | urinary metabolomics study on the protective role of orthosiphon stamineus in streptozotocin induced diabetes mellitus in rats via 1h nmr spectroscopy |
| topic | Orthosiphon Stamineus Java tea Metabolomics Diabetes mellitus 1H NMR spectroscopy |
| url | http://link.springer.com/article/10.1186/s12906-017-1777-1 |
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