Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopT
Symbiotic nitrogen fixation provides most of the nitrogen required for soybean growth. Rhizobial nodulation outer proteins (Nops) have been reported to influence host specificity during symbiosis establishment. However, the host proteins that interact with Nops remain unknown. In this study, we gene...
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2022-04-01
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| author | Hejia Ni Yang Peng Jinhui Wang Jing Wang Yantong Yuan Tingting Fu Zikun Zhu Jialin Zhang Xipeng Pan Zhuoling Cui Chunyan Liu Qingshan Chen Dawei Xin |
| author_facet | Hejia Ni Yang Peng Jinhui Wang Jing Wang Yantong Yuan Tingting Fu Zikun Zhu Jialin Zhang Xipeng Pan Zhuoling Cui Chunyan Liu Qingshan Chen Dawei Xin |
| author_sort | Hejia Ni |
| collection | DOAJ |
| description | Symbiotic nitrogen fixation provides most of the nitrogen required for soybean growth. Rhizobial nodulation outer proteins (Nops) have been reported to influence host specificity during symbiosis establishment. However, the host proteins that interact with Nops remain unknown. In this study, we generated <i>Sinorhizobium fredii</i> HH103 mutants (HH103Ω<i>NopL</i>, HH103Ω<i>NopT</i>, and HH103Ω<i>NopL</i>Ω<i>NopT</i>) and analysed the nodule number (NN) and nodule dry weight (NDW) of 12 soybean germplasms after inoculation with wild-type <i>S. fredii</i> HH103 or the mutant strains. The analysis of chromosome segment substitution lines revealed quantitative trait loci (QTLs) associated with NopL and NopT interactions. A total of 22 QTLs for the 2 nodule traits were detected and mapped to 12 different chromosomes in the soybean genome. Eight and fifteen QTLs were found to be associated with NN and NDW, respectively. Furthermore, 17 candidate genes were selected for further analyses. Considering the results of reverse-transcription quantitative PCR, we propose that the protein products of these 17 candidate genes interact with NopL and NopT. |
| first_indexed | 2024-03-09T11:16:41Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T11:16:41Z |
| publishDate | 2022-04-01 |
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| series | Agronomy |
| spelling | doaj.art-365106f37eee48119358fc9ece2a1c6d2023-12-01T00:29:15ZengMDPI AGAgronomy2073-43952022-04-0112494610.3390/agronomy12040946Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopTHejia Ni0Yang Peng1Jinhui Wang2Jing Wang3Yantong Yuan4Tingting Fu5Zikun Zhu6Jialin Zhang7Xipeng Pan8Zhuoling Cui9Chunyan Liu10Qingshan Chen11Dawei Xin12Key Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaKey Laboratory of Soybean Biology of the Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, College of Agriculture, Northeast Agricultural University, Harbin 150036, ChinaSymbiotic nitrogen fixation provides most of the nitrogen required for soybean growth. Rhizobial nodulation outer proteins (Nops) have been reported to influence host specificity during symbiosis establishment. However, the host proteins that interact with Nops remain unknown. In this study, we generated <i>Sinorhizobium fredii</i> HH103 mutants (HH103Ω<i>NopL</i>, HH103Ω<i>NopT</i>, and HH103Ω<i>NopL</i>Ω<i>NopT</i>) and analysed the nodule number (NN) and nodule dry weight (NDW) of 12 soybean germplasms after inoculation with wild-type <i>S. fredii</i> HH103 or the mutant strains. The analysis of chromosome segment substitution lines revealed quantitative trait loci (QTLs) associated with NopL and NopT interactions. A total of 22 QTLs for the 2 nodule traits were detected and mapped to 12 different chromosomes in the soybean genome. Eight and fifteen QTLs were found to be associated with NN and NDW, respectively. Furthermore, 17 candidate genes were selected for further analyses. Considering the results of reverse-transcription quantitative PCR, we propose that the protein products of these 17 candidate genes interact with NopL and NopT.https://www.mdpi.com/2073-4395/12/4/946symbiosistype III secretion system effectorsoybeanquantitative trait locusNopLNopT |
| spellingShingle | Hejia Ni Yang Peng Jinhui Wang Jing Wang Yantong Yuan Tingting Fu Zikun Zhu Jialin Zhang Xipeng Pan Zhuoling Cui Chunyan Liu Qingshan Chen Dawei Xin Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopT Agronomy symbiosis type III secretion system effector soybean quantitative trait locus NopL NopT |
| title | Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopT |
| title_full | Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopT |
| title_fullStr | Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopT |
| title_full_unstemmed | Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopT |
| title_short | Mapping of Quantitative Trait Loci Underlying Nodule Traits in Soybean (<i>Glycine max</i> (L.) Merr.) and Identification of Genes Whose Expression Is Affected by the <i>Sinorhizobium fredii</i> HH103 Effector Proteins NopL and NopT |
| title_sort | mapping of quantitative trait loci underlying nodule traits in soybean i glycine max i l merr and identification of genes whose expression is affected by the i sinorhizobium fredii i hh103 effector proteins nopl and nopt |
| topic | symbiosis type III secretion system effector soybean quantitative trait locus NopL NopT |
| url | https://www.mdpi.com/2073-4395/12/4/946 |
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