A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos
The objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR). Using alkaline method of DNA extraction from whole blood and feather bulb of adults, and tissue samples from...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2008-01-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/45/1/45_45_75/_pdf/-char/en |
| _version_ | 1797816238901035008 |
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| author | Santosh Haunshi Arunav Pattanayak Subhasis Bandyopadhaya Sharat C. Saxena Kamal M. Bujarbaruah |
| author_facet | Santosh Haunshi Arunav Pattanayak Subhasis Bandyopadhaya Sharat C. Saxena Kamal M. Bujarbaruah |
| author_sort | Santosh Haunshi |
| collection | DOAJ |
| description | The objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR). Using alkaline method of DNA extraction from whole blood and feather bulb of adults, and tissue samples from embryos, the present study demonstrated that identification of sex by multiplex PCR protocol is simplest, safer, faster and inexpensive. Multiplex PCR was used to amplify W chromosome specific 481-bp fragment in female and 256bp fragment of 18S ribosomal gene in both male and female chicken. DNA samples were prepared by conventional phenol-chloroform-iso-amyl alcohol (PCI) method, modified PCI method, wizard genomic DNA purification kit and a simple alkaline method from blood samples and feather bulbs of adults and tissue samples of embryos. The protocol successfully identified sex of embryos and White Leghorn (Exotic), indigenous and Vanaraja (Hybrid of exotic and indigenous) varieties of chicken. Sequence comparison of W chromosome specific PCR products amplified from these three varieties showed no difference among them. |
| first_indexed | 2024-03-13T08:34:47Z |
| format | Article |
| id | doaj.art-365123c726f44adc9d7ee3be6f30b08e |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:34:47Z |
| publishDate | 2008-01-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-365123c726f44adc9d7ee3be6f30b08e2023-05-31T00:32:44ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862008-01-01451758110.2141/jpsa.45.75jpsaA Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken EmbryosSantosh Haunshi0Arunav Pattanayak1Subhasis Bandyopadhaya2Sharat C. Saxena3Kamal M. Bujarbaruah4ICAR Research Complex for N.E.H. Region, IndiaICAR Research Complex for N.E.H. Region, IndiaICAR Research Complex for N.E.H. Region, IndiaICAR Research Complex for N.E.H. Region, IndiaICAR Research Complex for N.E.H. Region, IndiaThe objective of this study was to develop a simple and quick DNA extraction procedure for rapid diagnosis of sex of chicken and its embryos using multiplex polymerase chain reaction (PCR). Using alkaline method of DNA extraction from whole blood and feather bulb of adults, and tissue samples from embryos, the present study demonstrated that identification of sex by multiplex PCR protocol is simplest, safer, faster and inexpensive. Multiplex PCR was used to amplify W chromosome specific 481-bp fragment in female and 256bp fragment of 18S ribosomal gene in both male and female chicken. DNA samples were prepared by conventional phenol-chloroform-iso-amyl alcohol (PCI) method, modified PCI method, wizard genomic DNA purification kit and a simple alkaline method from blood samples and feather bulbs of adults and tissue samples of embryos. The protocol successfully identified sex of embryos and White Leghorn (Exotic), indigenous and Vanaraja (Hybrid of exotic and indigenous) varieties of chicken. Sequence comparison of W chromosome specific PCR products amplified from these three varieties showed no difference among them.https://www.jstage.jst.go.jp/article/jpsa/45/1/45_45_75/_pdf/-char/enalkalinechickendna extractionfeathersexing |
| spellingShingle | Santosh Haunshi Arunav Pattanayak Subhasis Bandyopadhaya Sharat C. Saxena Kamal M. Bujarbaruah A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos The Journal of Poultry Science alkaline chicken dna extraction feather sexing |
| title | A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos |
| title_full | A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos |
| title_fullStr | A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos |
| title_full_unstemmed | A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos |
| title_short | A Simple and Quick DNA Extraction Procedure for Rapid Diagnosis of Sex of Chicken and Chicken Embryos |
| title_sort | simple and quick dna extraction procedure for rapid diagnosis of sex of chicken and chicken embryos |
| topic | alkaline chicken dna extraction feather sexing |
| url | https://www.jstage.jst.go.jp/article/jpsa/45/1/45_45_75/_pdf/-char/en |
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