Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma
Abstract Background Pathway mutations have been calculated to predict the poor prognosis and immunotherapy resistance in head and neck squamous cell carcinoma (HNSCC). To uncover the unique markers predicting prognosis and immune therapy response, the accurate quantification of pathway mutations are...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Medical Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12920-024-01818-6 |
| _version_ | 1797272885451030528 |
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| author | Yuhong Huang Han Liu Bo Liu Xiaoyan Chen Danya Li Junyuan Xue Nan Li Lei Zhu Liu Yang Jing Xiao Chao Liu |
| author_facet | Yuhong Huang Han Liu Bo Liu Xiaoyan Chen Danya Li Junyuan Xue Nan Li Lei Zhu Liu Yang Jing Xiao Chao Liu |
| author_sort | Yuhong Huang |
| collection | DOAJ |
| description | Abstract Background Pathway mutations have been calculated to predict the poor prognosis and immunotherapy resistance in head and neck squamous cell carcinoma (HNSCC). To uncover the unique markers predicting prognosis and immune therapy response, the accurate quantification of pathway mutations are required to evaluate epithelial-mesenchymal transition (EMT) and immune escape. Yet, there is a lack of score to accurately quantify pathway mutations. Material and methods Firstly, we proposed Individualized Weighted Hallmark Gene Set Mutation Burden (IWHMB, https://github.com/YuHongHuang-lab/IWHMB ) which integrated pathway structure information and eliminated the interference of global Tumor Mutation Burden to accurately quantify pathway mutations. Subsequently, to further elucidate the association of IWHMB with EMT and immune escape, support vector machine regression model was used to identify IWHMB-related transcriptomic features (IRG), while Adversarially Regularized Graph Autoencoder (ARVGA) was used to further resolve IRG network features. Finally, Random walk with restart algorithm was used to identify biomarkers for predicting ICI response. Results We quantified the HNSCC pathway mutation signatures and identified pathway mutation subtypes using IWHMB. The IWHMB-related transcriptomic features (IRG) identified by support vector machine regression were divided into 5 communities by ARVGA, among which the Community 1 enriching malignant mesenchymal components promoted EMT dynamically and regulated immune patterns associated with ICI responses. Bridge Hub Gene (BHG) identified by random walk with restart was key to IWHMB in EMT and immune escape, thus, more predictive for ICI response than other 70 public signatures. Conclusion In summary, the novel pathway mutation scoring-IWHMB suggested that the elevated malignancy mediated by pathway mutations is a major cause of poor prognosis and immunotherapy failure in HNSCC, and is capable of identifying novel biomarkers to predict immunotherapy response. |
| first_indexed | 2024-03-07T14:35:42Z |
| format | Article |
| id | doaj.art-36524003beee48e79088d94fdd706b60 |
| institution | Directory Open Access Journal |
| issn | 1755-8794 |
| language | English |
| last_indexed | 2024-03-07T14:35:42Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj.art-36524003beee48e79088d94fdd706b602024-03-05T20:39:14ZengBMCBMC Medical Genomics1755-87942024-02-0117112610.1186/s12920-024-01818-6Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinomaYuhong Huang0Han Liu1Bo Liu2Xiaoyan Chen3Danya Li4Junyuan Xue5Nan Li6Lei Zhu7Liu Yang8Jing Xiao9Chao Liu10Department of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyInstitute for Genome Engineered Animal Models of Human Diseases, Dalian Medical UniversityDepartment of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyDepartment of Oral Pathology, Dalian Medical University School of StomatologyAbstract Background Pathway mutations have been calculated to predict the poor prognosis and immunotherapy resistance in head and neck squamous cell carcinoma (HNSCC). To uncover the unique markers predicting prognosis and immune therapy response, the accurate quantification of pathway mutations are required to evaluate epithelial-mesenchymal transition (EMT) and immune escape. Yet, there is a lack of score to accurately quantify pathway mutations. Material and methods Firstly, we proposed Individualized Weighted Hallmark Gene Set Mutation Burden (IWHMB, https://github.com/YuHongHuang-lab/IWHMB ) which integrated pathway structure information and eliminated the interference of global Tumor Mutation Burden to accurately quantify pathway mutations. Subsequently, to further elucidate the association of IWHMB with EMT and immune escape, support vector machine regression model was used to identify IWHMB-related transcriptomic features (IRG), while Adversarially Regularized Graph Autoencoder (ARVGA) was used to further resolve IRG network features. Finally, Random walk with restart algorithm was used to identify biomarkers for predicting ICI response. Results We quantified the HNSCC pathway mutation signatures and identified pathway mutation subtypes using IWHMB. The IWHMB-related transcriptomic features (IRG) identified by support vector machine regression were divided into 5 communities by ARVGA, among which the Community 1 enriching malignant mesenchymal components promoted EMT dynamically and regulated immune patterns associated with ICI responses. Bridge Hub Gene (BHG) identified by random walk with restart was key to IWHMB in EMT and immune escape, thus, more predictive for ICI response than other 70 public signatures. Conclusion In summary, the novel pathway mutation scoring-IWHMB suggested that the elevated malignancy mediated by pathway mutations is a major cause of poor prognosis and immunotherapy failure in HNSCC, and is capable of identifying novel biomarkers to predict immunotherapy response.https://doi.org/10.1186/s12920-024-01818-6Pathway Mutation Burden (PMB)Tumor Mutation Burden (TMB)Functional genomicsPolyomicsTranscriptome |
| spellingShingle | Yuhong Huang Han Liu Bo Liu Xiaoyan Chen Danya Li Junyuan Xue Nan Li Lei Zhu Liu Yang Jing Xiao Chao Liu Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma BMC Medical Genomics Pathway Mutation Burden (PMB) Tumor Mutation Burden (TMB) Functional genomics Polyomics Transcriptome |
| title | Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma |
| title_full | Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma |
| title_fullStr | Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma |
| title_full_unstemmed | Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma |
| title_short | Quantified pathway mutations associate epithelial-mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma |
| title_sort | quantified pathway mutations associate epithelial mesenchymal transition and immune escape with poor prognosis and immunotherapy resistance of head and neck squamous cell carcinoma |
| topic | Pathway Mutation Burden (PMB) Tumor Mutation Burden (TMB) Functional genomics Polyomics Transcriptome |
| url | https://doi.org/10.1186/s12920-024-01818-6 |
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