New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients
Osteoarthritis (OA) is a degenerative condition of the articular cartilage with chronic low-grade inflammation. Monocytes have a fundamental role in the progression of OA, given their implication in inflammatory responses and their capacity to differentiate into bone-resorbing osteoclasts (OCLs). Th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1710 |
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| author | Paloma Guillem-Llobat Marta Marín Matthieu Rouleau Antonio Silvestre Claudine Blin-Wakkach María Luisa Ferrándiz María Isabel Guillén Lidia Ibáñez |
| author_facet | Paloma Guillem-Llobat Marta Marín Matthieu Rouleau Antonio Silvestre Claudine Blin-Wakkach María Luisa Ferrándiz María Isabel Guillén Lidia Ibáñez |
| author_sort | Paloma Guillem-Llobat |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a degenerative condition of the articular cartilage with chronic low-grade inflammation. Monocytes have a fundamental role in the progression of OA, given their implication in inflammatory responses and their capacity to differentiate into bone-resorbing osteoclasts (OCLs). This observational–experimental study attempted to better understand the molecular pathogenesis of OA through the examination of osteoclast progenitor (OCP) cells from both OA patients and healthy individuals (25 OA patients and healthy samples). The expression of osteoclastogenic and inflammatory genes was analyzed using RT-PCR. The OA monocytes expressed significantly higher levels of CD16, CD115, TLR2, Mincle, Dentin-1, and CCR2 mRNAs. Moreover, a flow cytometry analysis showed a significantly higher surface expression of the CD16 and CD115 receptors in OA vs. healthy monocytes, as well as a difference in the distribution of monocyte subsets. Additionally, the OA monocytes showed a greater osteoclast differentiation capacity and an enhanced response to an inflammatory stimulus. The results of this study demonstrate the existence of significant differences between the OCPs of OA patients and those of healthy subjects. These differences could contribute to a greater understanding of the molecular pathogenesis of OA and to the identification of new biomarkers and potential drug targets for OA. |
| first_indexed | 2024-03-08T03:55:25Z |
| format | Article |
| id | doaj.art-365a2fabc03a48b7bb6f2154308496b6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:55:25Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-365a2fabc03a48b7bb6f2154308496b62024-02-09T15:14:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01253171010.3390/ijms25031710New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis PatientsPaloma Guillem-Llobat0Marta Marín1Matthieu Rouleau2Antonio Silvestre3Claudine Blin-Wakkach4María Luisa Ferrándiz5María Isabel Guillén6Lidia Ibáñez7Department of Biomedical Science, Cardenal Herrera-CEU University, 46115 Valencia, SpainDepartment of Pharmacy, Cardenal Herrera-CEU University, 46115 Valencia, SpainLaboratory of Molecular PhysioMedicine, UMR 7370, National Centre for Scientific Research, Côte d’Azur University, 06107 Nice, FranceService of Orthopedic Surgery and Traumatology, University Clinical Hospital, 46010 Valencia, SpainLaboratory of Molecular PhysioMedicine, UMR 7370, National Centre for Scientific Research, Côte d’Azur University, 06107 Nice, FranceInteruniversity Research Institute for Molecular Recognition and Technological Development (IDM), Polytechnic University of Valencia and University of Valencia, 46022 Valencia, SpainDepartment of Pharmacy, Cardenal Herrera-CEU University, 46115 Valencia, SpainDepartment of Pharmacy, Cardenal Herrera-CEU University, 46115 Valencia, SpainOsteoarthritis (OA) is a degenerative condition of the articular cartilage with chronic low-grade inflammation. Monocytes have a fundamental role in the progression of OA, given their implication in inflammatory responses and their capacity to differentiate into bone-resorbing osteoclasts (OCLs). This observational–experimental study attempted to better understand the molecular pathogenesis of OA through the examination of osteoclast progenitor (OCP) cells from both OA patients and healthy individuals (25 OA patients and healthy samples). The expression of osteoclastogenic and inflammatory genes was analyzed using RT-PCR. The OA monocytes expressed significantly higher levels of CD16, CD115, TLR2, Mincle, Dentin-1, and CCR2 mRNAs. Moreover, a flow cytometry analysis showed a significantly higher surface expression of the CD16 and CD115 receptors in OA vs. healthy monocytes, as well as a difference in the distribution of monocyte subsets. Additionally, the OA monocytes showed a greater osteoclast differentiation capacity and an enhanced response to an inflammatory stimulus. The results of this study demonstrate the existence of significant differences between the OCPs of OA patients and those of healthy subjects. These differences could contribute to a greater understanding of the molecular pathogenesis of OA and to the identification of new biomarkers and potential drug targets for OA.https://www.mdpi.com/1422-0067/25/3/1710bone remodelingmonocyteosteoclast progenitorosteoarthritisinflammation |
| spellingShingle | Paloma Guillem-Llobat Marta Marín Matthieu Rouleau Antonio Silvestre Claudine Blin-Wakkach María Luisa Ferrándiz María Isabel Guillén Lidia Ibáñez New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients International Journal of Molecular Sciences bone remodeling monocyte osteoclast progenitor osteoarthritis inflammation |
| title | New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients |
| title_full | New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients |
| title_fullStr | New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients |
| title_full_unstemmed | New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients |
| title_short | New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients |
| title_sort | new insights into the pro inflammatory and osteoclastogenic profile of circulating monocytes in osteoarthritis patients |
| topic | bone remodeling monocyte osteoclast progenitor osteoarthritis inflammation |
| url | https://www.mdpi.com/1422-0067/25/3/1710 |
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