Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin
Abstract The simultaneous and sequential dual elicitation effect on plumbagin production in Plumbago indica L. root cultures, revealed that combination of chitosan (150 mg L−1) with ʟ-alanine (5 mM) or methyl-β-cyclodextrin (MCD; 2 mM) significantly increased plumbagin production, but in the differe...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-02-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-020-0298-9 |
| _version_ | 1818037230104477696 |
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| author | Amit Jaisi Pharkphoom Panichayupakaranant |
| author_facet | Amit Jaisi Pharkphoom Panichayupakaranant |
| author_sort | Amit Jaisi |
| collection | DOAJ |
| description | Abstract The simultaneous and sequential dual elicitation effect on plumbagin production in Plumbago indica L. root cultures, revealed that combination of chitosan (150 mg L−1) with ʟ-alanine (5 mM) or methyl-β-cyclodextrin (MCD; 2 mM) significantly increased plumbagin production, but in the different treatment manners. The simultaneous treatment using chitosan + ʟ-alanine on a 14-day-old culture enhanced plumbagin production to 14.62 mg g−1 DW, while the sequential additions of MCD to a 12-day-old culture followed by chitosan after 48 h enhanced production of plumbagin to 14.33 mg g−1 DW. The plumbagin productivity from both treatments were up to 1.3- and 8-fold higher than the chitosan treated (10.93 mg g−1 DW) and untreated root cultures (1.76 mg g−1 DW). Moreover, the present studies provided new information on the effect of simultaneous and sequential elicitation on plumbagin-producing P. indica root cultures using chitosan in combinations with MCD or ʟ-alanine. |
| first_indexed | 2024-12-10T07:23:32Z |
| format | Article |
| id | doaj.art-365b9d0f574d43ce8d2794e07e5777eb |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-10T07:23:32Z |
| publishDate | 2020-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-365b9d0f574d43ce8d2794e07e5777eb2022-12-22T01:57:45ZengSpringerOpenBioresources and Bioprocessing2197-43652020-02-01711710.1186/s40643-020-0298-9Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrinAmit Jaisi0Pharkphoom Panichayupakaranant1School of Pharmacy, Walailak UniversityPhytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla UniversityAbstract The simultaneous and sequential dual elicitation effect on plumbagin production in Plumbago indica L. root cultures, revealed that combination of chitosan (150 mg L−1) with ʟ-alanine (5 mM) or methyl-β-cyclodextrin (MCD; 2 mM) significantly increased plumbagin production, but in the different treatment manners. The simultaneous treatment using chitosan + ʟ-alanine on a 14-day-old culture enhanced plumbagin production to 14.62 mg g−1 DW, while the sequential additions of MCD to a 12-day-old culture followed by chitosan after 48 h enhanced production of plumbagin to 14.33 mg g−1 DW. The plumbagin productivity from both treatments were up to 1.3- and 8-fold higher than the chitosan treated (10.93 mg g−1 DW) and untreated root cultures (1.76 mg g−1 DW). Moreover, the present studies provided new information on the effect of simultaneous and sequential elicitation on plumbagin-producing P. indica root cultures using chitosan in combinations with MCD or ʟ-alanine.http://link.springer.com/article/10.1186/s40643-020-0298-9ʟ-AlanineChitosanDual elicitationMethyl-β-cyclodextrinPlumbaginPlumbago indica |
| spellingShingle | Amit Jaisi Pharkphoom Panichayupakaranant Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin Bioresources and Bioprocessing ʟ-Alanine Chitosan Dual elicitation Methyl-β-cyclodextrin Plumbagin Plumbago indica |
| title | Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin |
| title_full | Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin |
| title_fullStr | Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin |
| title_full_unstemmed | Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin |
| title_short | Enhanced plumbagin production in Plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with ʟ-alanine and methyl-β-cyclodextrin |
| title_sort | enhanced plumbagin production in plumbago indica root culture by simultaneous and sequential dual elicitations using chitosan with l alanine and methyl β cyclodextrin |
| topic | ʟ-Alanine Chitosan Dual elicitation Methyl-β-cyclodextrin Plumbagin Plumbago indica |
| url | http://link.springer.com/article/10.1186/s40643-020-0298-9 |
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