Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls
Our previous studies showed that hydrogen-rich water (HRW) promoted the biosynthesis of anthocyanin under UV-A in radish. However, molecular mechanism involved in the regulation of the anthocyanin biosynthesis is still unclear. In this study, the role of calcium (Ca2+) in HRW-promoted anthocyanin bi...
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01020/full |
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| author | Xiaoyan Zhang Xiaoyan Zhang Xiaoyan Zhang Junyu Wei Yifan Huang Wenbiao Shen Xin Chen Chungui Lu Nana Su Jin Cui |
| author_facet | Xiaoyan Zhang Xiaoyan Zhang Xiaoyan Zhang Junyu Wei Yifan Huang Wenbiao Shen Xin Chen Chungui Lu Nana Su Jin Cui |
| author_sort | Xiaoyan Zhang |
| collection | DOAJ |
| description | Our previous studies showed that hydrogen-rich water (HRW) promoted the biosynthesis of anthocyanin under UV-A in radish. However, molecular mechanism involved in the regulation of the anthocyanin biosynthesis is still unclear. In this study, the role of calcium (Ca2+) in HRW-promoted anthocyanin biosynthesis in radish sprouts hypocotyls under UV-A was investigated. The results showed that a positive effect of HRW on the content of cytosolic calcium and anthocyanin accumulation, mimicking the effects of induced CaCl2. Exogenous addition of Ca2+ chelator bis (β-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) and inositol 1,4,5-trisphosphate (IP3) synthesis inhibitor neomycin partially reversed the facilitated effect of HRW. The positive effects of HRW on activity of anthocyanin biosynthetic-enzymes (L-phenylalanine ammonia-lyase, PAL; chalcone isomerase, CHI; dihydroflavonol 4-reductase, DFR and UDP glc-flavonoid 3-O-glucosyl transferase, UFGT) were reversed by EGTA and neomycin. Further tests confirmed that the upregulation of anthocyanin biosynthetic related genes induced by HRW was substantially inhibited by calcium antagonists. The possible involvement of CaM in HRW-regulated anthocyanin biosynthesis was also preliminarily investigated in this study. Taken together, our results indicate that IP3-dependent calcium signaling pathway might be involved in HRW-regulated anthocyanin biosynthesis under UV-A irradiation. |
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| last_indexed | 2024-12-11T01:07:17Z |
| publishDate | 2018-07-01 |
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| spelling | doaj.art-365d402547f54149adca11073c3deeb42022-12-22T01:26:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-07-01910.3389/fpls.2018.01020398102Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts HypocotylsXiaoyan Zhang0Xiaoyan Zhang1Xiaoyan Zhang2Junyu Wei3Yifan Huang4Wenbiao Shen5Xin Chen6Chungui Lu7Nana Su8Jin Cui9College of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Animal, Rural and Environmental Sciences, Nottingham Trent University, Nottingham, United KingdomCollege of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaInstitute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaSchool of Animal, Rural and Environmental Sciences, Nottingham Trent University, Nottingham, United KingdomCollege of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaCollege of Life Sciences, Nanjing Agricultural University, Nanjing, ChinaOur previous studies showed that hydrogen-rich water (HRW) promoted the biosynthesis of anthocyanin under UV-A in radish. However, molecular mechanism involved in the regulation of the anthocyanin biosynthesis is still unclear. In this study, the role of calcium (Ca2+) in HRW-promoted anthocyanin biosynthesis in radish sprouts hypocotyls under UV-A was investigated. The results showed that a positive effect of HRW on the content of cytosolic calcium and anthocyanin accumulation, mimicking the effects of induced CaCl2. Exogenous addition of Ca2+ chelator bis (β-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) and inositol 1,4,5-trisphosphate (IP3) synthesis inhibitor neomycin partially reversed the facilitated effect of HRW. The positive effects of HRW on activity of anthocyanin biosynthetic-enzymes (L-phenylalanine ammonia-lyase, PAL; chalcone isomerase, CHI; dihydroflavonol 4-reductase, DFR and UDP glc-flavonoid 3-O-glucosyl transferase, UFGT) were reversed by EGTA and neomycin. Further tests confirmed that the upregulation of anthocyanin biosynthetic related genes induced by HRW was substantially inhibited by calcium antagonists. The possible involvement of CaM in HRW-regulated anthocyanin biosynthesis was also preliminarily investigated in this study. Taken together, our results indicate that IP3-dependent calcium signaling pathway might be involved in HRW-regulated anthocyanin biosynthesis under UV-A irradiation.https://www.frontiersin.org/article/10.3389/fpls.2018.01020/fullcalciumhydrogen-rich waterradish sproutsanthocyaninUV-A |
| spellingShingle | Xiaoyan Zhang Xiaoyan Zhang Xiaoyan Zhang Junyu Wei Yifan Huang Wenbiao Shen Xin Chen Chungui Lu Nana Su Jin Cui Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls Frontiers in Plant Science calcium hydrogen-rich water radish sprouts anthocyanin UV-A |
| title | Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls |
| title_full | Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls |
| title_fullStr | Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls |
| title_full_unstemmed | Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls |
| title_short | Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls |
| title_sort | increased cytosolic calcium contributes to hydrogen rich water promoted anthocyanin biosynthesis under uv a irradiation in radish sprouts hypocotyls |
| topic | calcium hydrogen-rich water radish sprouts anthocyanin UV-A |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01020/full |
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