Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair
Silk fibroin (SF) is considered biocompatible and biodegradable for osteochondral repair. However, it lacks a bioactive domain for cell adhesion, proliferation and differentiation, limiting its therapeutic efficacy. To revamp SF as a biomimicking and bioactive microenvironment to regulate cell behav...
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KeAi Communications Co., Ltd.
2021-03-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X20302097 |
| _version_ | 1797204309617672192 |
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| author | Zhuoyue Chen Qiang Zhang Hongmin Li Qi Wei Xin Zhao Fulin Chen |
| author_facet | Zhuoyue Chen Qiang Zhang Hongmin Li Qi Wei Xin Zhao Fulin Chen |
| author_sort | Zhuoyue Chen |
| collection | DOAJ |
| description | Silk fibroin (SF) is considered biocompatible and biodegradable for osteochondral repair. However, it lacks a bioactive domain for cell adhesion, proliferation and differentiation, limiting its therapeutic efficacy. To revamp SF as a biomimicking and bioactive microenvironment to regulate cell behaviours, we engineered an elastin-like polypeptide (ELP, Val-Pro-Gly-Xaa-Gly) to modify SF fibers via simple and green dehydrothermal (DHT) treatment. Our results demonstrated that the ELP successfully bound to SF, and the scaffold was reinforced by the fusion of the silk fiber intersections with ELP (S-ELP-DHT) via the DHT treatment. Both bone mesenchymal stem cells (BMSCs) and chondrocytes exhibited improved spreading and proliferation on the S-ELP-DHT scaffolds. The ex vivo and in vivo experiments further demonstrated enhanced mature bone and cartilage tissue formation using the S-ELP-DHT scaffolds compared to the naked SF scaffolds. These results indicated that a recombinant ELP-modified silk scaffold can mimic three-dimensional (3D) cell microenvironment, and improve bone and cartilage regeneration. We envision that our scaffolds have huge clinical potential for osteochondral repair. |
| first_indexed | 2024-04-24T08:33:11Z |
| format | Article |
| id | doaj.art-36612f261c044752b510ab29555e6978 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:33:11Z |
| publishDate | 2021-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-36612f261c044752b510ab29555e69782024-04-16T18:25:36ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-03-0163589601Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repairZhuoyue Chen0Qiang Zhang1Hongmin Li2Qi Wei3Xin Zhao4Fulin Chen5Provincial Key Laboratory of Biotechnology of Shaanxi, Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Faculty of Life Science, Northwest University, 229 North TaiBai Road, Xi'an, Shaanxi Province, 710069, ChinaDepartment of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, ChinaProvincial Key Laboratory of Biotechnology of Shaanxi, Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Faculty of Life Science, Northwest University, 229 North TaiBai Road, Xi'an, Shaanxi Province, 710069, ChinaProvincial Key Laboratory of Biotechnology of Shaanxi, Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Faculty of Life Science, Northwest University, 229 North TaiBai Road, Xi'an, Shaanxi Province, 710069, ChinaDepartment of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; Corresponding author.Provincial Key Laboratory of Biotechnology of Shaanxi, Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Faculty of Life Science, Northwest University, 229 North TaiBai Road, Xi'an, Shaanxi Province, 710069, China; Corresponding author.Silk fibroin (SF) is considered biocompatible and biodegradable for osteochondral repair. However, it lacks a bioactive domain for cell adhesion, proliferation and differentiation, limiting its therapeutic efficacy. To revamp SF as a biomimicking and bioactive microenvironment to regulate cell behaviours, we engineered an elastin-like polypeptide (ELP, Val-Pro-Gly-Xaa-Gly) to modify SF fibers via simple and green dehydrothermal (DHT) treatment. Our results demonstrated that the ELP successfully bound to SF, and the scaffold was reinforced by the fusion of the silk fiber intersections with ELP (S-ELP-DHT) via the DHT treatment. Both bone mesenchymal stem cells (BMSCs) and chondrocytes exhibited improved spreading and proliferation on the S-ELP-DHT scaffolds. The ex vivo and in vivo experiments further demonstrated enhanced mature bone and cartilage tissue formation using the S-ELP-DHT scaffolds compared to the naked SF scaffolds. These results indicated that a recombinant ELP-modified silk scaffold can mimic three-dimensional (3D) cell microenvironment, and improve bone and cartilage regeneration. We envision that our scaffolds have huge clinical potential for osteochondral repair.http://www.sciencedirect.com/science/article/pii/S2452199X20302097Silk fiberElastin-like polypeptideBone repairCartilage repair |
| spellingShingle | Zhuoyue Chen Qiang Zhang Hongmin Li Qi Wei Xin Zhao Fulin Chen Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair Bioactive Materials Silk fiber Elastin-like polypeptide Bone repair Cartilage repair |
| title | Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair |
| title_full | Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair |
| title_fullStr | Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair |
| title_full_unstemmed | Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair |
| title_short | Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair |
| title_sort | elastin like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair |
| topic | Silk fiber Elastin-like polypeptide Bone repair Cartilage repair |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X20302097 |
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