Which Stage of Mouse Embryos Is More Appropriate for Vitrification?
Background Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carr...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2017-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45402_72c86c7f82c3ca92caababf03b45456d.pdf |
| _version_ | 1811332993146421248 |
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| author | Nasibeh Ghandy Abbas Ali Karimpur Malekshah |
| author_facet | Nasibeh Ghandy Abbas Ali Karimpur Malekshah |
| author_sort | Nasibeh Ghandy |
| collection | DOAJ |
| description | Background Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, morula and blastocyst stage embryos and subsequent blast formation and hatching after thawing. Materials and Methods In this experimental study, 2-cell embryos were obtained from the oviducts of super ovulated female NMRI mice. Some embryos were randomly selected and vitrified through a two-step media protocol and cryotop. Other embryos were cultured to assess their development. During the ensuing days, some of these cultured embryos were vitrified at 4-cell, 8-cell, morula and blastocyst stages. After 10 to 14 days, the embryos were thawed to assess their survival and also cultured to determine the rate of blastocyst formation and hatching. The results were analyzed using one-way ANOVA and Tukey’s post-hoc tests. Results There was no significant difference in the survival rates of vitrified embryos at 2-cell, 4-cell, 8-cell, morula and blastocyst stages after thawing (P > 0.05). The blastocyst formation rate of vitrified 8-cell embryos was significantly higher than that of 2-cell embryos (P < 0.05). The hatching rate of vitrified 4-cell, 8-cell and blastocysts were significantly higher than that of 2-cell embryos (P < 0.05). Conclusion Vitrification is suitable for cryopreservation of all stages of mouse embryonic development. However, the best tolerance for vitrification was observed at 4and 8-cell stages of development. Accordingly, the development of vitrified embryos to blastocysts, following thawing, was most efficacious for 4 and 8-cell embryos. Compared to mouse 2-cell embryos, embryos vitrified as blastocysts had the highest rate of hatching. |
| first_indexed | 2024-04-13T16:45:22Z |
| format | Article |
| id | doaj.art-3663ee762acd487f8896c0b4c3ab3618 |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-04-13T16:45:22Z |
| publishDate | 2017-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-3663ee762acd487f8896c0b4c3ab36182022-12-22T02:39:06ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782017-01-0110435736210.22074/ijfs.2016.508645402Which Stage of Mouse Embryos Is More Appropriate for Vitrification?Nasibeh Ghandy0Abbas Ali Karimpur Malekshah1Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranMolecular and Cell Biology Research Center, Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IranBackground Vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. However, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. This study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, morula and blastocyst stage embryos and subsequent blast formation and hatching after thawing. Materials and Methods In this experimental study, 2-cell embryos were obtained from the oviducts of super ovulated female NMRI mice. Some embryos were randomly selected and vitrified through a two-step media protocol and cryotop. Other embryos were cultured to assess their development. During the ensuing days, some of these cultured embryos were vitrified at 4-cell, 8-cell, morula and blastocyst stages. After 10 to 14 days, the embryos were thawed to assess their survival and also cultured to determine the rate of blastocyst formation and hatching. The results were analyzed using one-way ANOVA and Tukey’s post-hoc tests. Results There was no significant difference in the survival rates of vitrified embryos at 2-cell, 4-cell, 8-cell, morula and blastocyst stages after thawing (P > 0.05). The blastocyst formation rate of vitrified 8-cell embryos was significantly higher than that of 2-cell embryos (P < 0.05). The hatching rate of vitrified 4-cell, 8-cell and blastocysts were significantly higher than that of 2-cell embryos (P < 0.05). Conclusion Vitrification is suitable for cryopreservation of all stages of mouse embryonic development. However, the best tolerance for vitrification was observed at 4and 8-cell stages of development. Accordingly, the development of vitrified embryos to blastocysts, following thawing, was most efficacious for 4 and 8-cell embryos. Compared to mouse 2-cell embryos, embryos vitrified as blastocysts had the highest rate of hatching.http://www.ijfs.ir/article_45402_72c86c7f82c3ca92caababf03b45456d.pdfvitrificationembryopreimplantation |
| spellingShingle | Nasibeh Ghandy Abbas Ali Karimpur Malekshah Which Stage of Mouse Embryos Is More Appropriate for Vitrification? International Journal of Fertility and Sterility vitrification embryo preimplantation |
| title | Which Stage of Mouse Embryos Is More Appropriate for Vitrification? |
| title_full | Which Stage of Mouse Embryos Is More Appropriate for Vitrification? |
| title_fullStr | Which Stage of Mouse Embryos Is More Appropriate for Vitrification? |
| title_full_unstemmed | Which Stage of Mouse Embryos Is More Appropriate for Vitrification? |
| title_short | Which Stage of Mouse Embryos Is More Appropriate for Vitrification? |
| title_sort | which stage of mouse embryos is more appropriate for vitrification |
| topic | vitrification embryo preimplantation |
| url | http://www.ijfs.ir/article_45402_72c86c7f82c3ca92caababf03b45456d.pdf |
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