Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni
ABSTRACT The survival strategies that Campylobacter jejuni (C. jejuni) employ throughout its transmission and infection life cycles remain largely elusive. Specifically, there is a lack of understanding about the posttranscriptional regulation of stress adaptations resulting from small noncoding RNA...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-06-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00203-22 |
| _version_ | 1811333182946017280 |
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| author | Stephen Li Jenna Lam Leonidas Souliotis Mohammad Tauqeer Alam Chrystala Constantinidou |
| author_facet | Stephen Li Jenna Lam Leonidas Souliotis Mohammad Tauqeer Alam Chrystala Constantinidou |
| author_sort | Stephen Li |
| collection | DOAJ |
| description | ABSTRACT The survival strategies that Campylobacter jejuni (C. jejuni) employ throughout its transmission and infection life cycles remain largely elusive. Specifically, there is a lack of understanding about the posttranscriptional regulation of stress adaptations resulting from small noncoding RNAs (sRNAs). Published C. jejuni sRNAs have been discovered in specific conditions but with limited insights into their biological activities. Many more sRNAs are yet to be discovered as they may be condition-dependent. Here, we have generated transcriptomic data from 21 host- and transmission-relevant conditions. The data uncovered transcription start sites, expression patterns and posttranscriptional regulation during various stress conditions. This data set helped predict a list of putative sRNAs. We further explored the sRNAs’ biological functions by integrating differential gene expression analysis, coexpression analysis, and genome-wide sRNA target prediction. The results showed that the C. jejuni gene expression was influenced primarily by nutrient deprivation and food storage conditions. Further exploration revealed a putative sRNA (CjSA21) that targeted tlp1 to 4 under food processing conditions. tlp1 to 4 are transcripts that encode methyl-accepting chemotaxis proteins (MCPs), which are responsible for chemosensing. These results suggested CjSA21 inhibits chemotaxis and promotes survival under food processing conditions. This study presents the broader research community with a comprehensive data set and highlights a novel sRNA as a potential chemotaxis inhibitor. IMPORTANCE The foodborne pathogen C. jejuni is a significant challenge for the global health care system. It is crucial to investigate C. jejuni posttranscriptional regulation by small RNAs (sRNAs) in order to understand how it adapts to different stress conditions. However, limited data are available for investigating sRNA activity under stress. In this study, we generate gene expression data of C. jejuni under 21 stress conditions. Our data analysis indicates that one of the novel sRNAs mediates the adaptation to food processing conditions. Results from our work shed light on the posttranscriptional regulation of C. jejuni and identify an sRNA associated with food safety. |
| first_indexed | 2024-04-13T16:49:12Z |
| format | Article |
| id | doaj.art-36644e70c24d49a9af5a005cc59905a6 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-13T16:49:12Z |
| publishDate | 2022-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-36644e70c24d49a9af5a005cc59905a62022-12-22T02:39:00ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-06-0110310.1128/spectrum.00203-22Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuniStephen Li0Jenna Lam1Leonidas Souliotis2Mohammad Tauqeer Alam3Chrystala Constantinidou4Warwick Medical School, University of Warwick, Coventry, United KingdomWarwick Medical School, University of Warwick, Coventry, United KingdomWarwick Medical School, University of Warwick, Coventry, United KingdomDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesWarwick Medical School, University of Warwick, Coventry, United KingdomABSTRACT The survival strategies that Campylobacter jejuni (C. jejuni) employ throughout its transmission and infection life cycles remain largely elusive. Specifically, there is a lack of understanding about the posttranscriptional regulation of stress adaptations resulting from small noncoding RNAs (sRNAs). Published C. jejuni sRNAs have been discovered in specific conditions but with limited insights into their biological activities. Many more sRNAs are yet to be discovered as they may be condition-dependent. Here, we have generated transcriptomic data from 21 host- and transmission-relevant conditions. The data uncovered transcription start sites, expression patterns and posttranscriptional regulation during various stress conditions. This data set helped predict a list of putative sRNAs. We further explored the sRNAs’ biological functions by integrating differential gene expression analysis, coexpression analysis, and genome-wide sRNA target prediction. The results showed that the C. jejuni gene expression was influenced primarily by nutrient deprivation and food storage conditions. Further exploration revealed a putative sRNA (CjSA21) that targeted tlp1 to 4 under food processing conditions. tlp1 to 4 are transcripts that encode methyl-accepting chemotaxis proteins (MCPs), which are responsible for chemosensing. These results suggested CjSA21 inhibits chemotaxis and promotes survival under food processing conditions. This study presents the broader research community with a comprehensive data set and highlights a novel sRNA as a potential chemotaxis inhibitor. IMPORTANCE The foodborne pathogen C. jejuni is a significant challenge for the global health care system. It is crucial to investigate C. jejuni posttranscriptional regulation by small RNAs (sRNAs) in order to understand how it adapts to different stress conditions. However, limited data are available for investigating sRNA activity under stress. In this study, we generate gene expression data of C. jejuni under 21 stress conditions. Our data analysis indicates that one of the novel sRNAs mediates the adaptation to food processing conditions. Results from our work shed light on the posttranscriptional regulation of C. jejuni and identify an sRNA associated with food safety.https://journals.asm.org/doi/10.1128/spectrum.00203-22C. jejunibioinformaticssRNAsignal transductiontranscriptional regulation |
| spellingShingle | Stephen Li Jenna Lam Leonidas Souliotis Mohammad Tauqeer Alam Chrystala Constantinidou Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni Microbiology Spectrum C. jejuni bioinformatics sRNA signal transduction transcriptional regulation |
| title | Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni |
| title_full | Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni |
| title_fullStr | Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni |
| title_full_unstemmed | Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni |
| title_short | Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni |
| title_sort | posttranscriptional regulation in response to different environmental stresses in campylobacter jejuni |
| topic | C. jejuni bioinformatics sRNA signal transduction transcriptional regulation |
| url | https://journals.asm.org/doi/10.1128/spectrum.00203-22 |
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