In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infection
Purpose To evaluate the results of a comparative analysis of the in vitro antimicrobial activity of polymer hydrogels and PMMA impregnated with antibiotics against test cultures of the leading pathogens of bone infection. Materials and methods A comparative analysis of the antimicrobial activity o...
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| Format: | Article |
| Language: | English |
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Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics
2022-12-01
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| Series: | Гений oртопедии |
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| _version_ | 1798006794606346240 |
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| author | Archil V. Tsiskarashvili Regina E. Melikova Tamara Ya. Pkhakadze Alexander A. Artyukhov Natalya V. Sokorova |
| author_facet | Archil V. Tsiskarashvili Regina E. Melikova Tamara Ya. Pkhakadze Alexander A. Artyukhov Natalya V. Sokorova |
| author_sort | Archil V. Tsiskarashvili |
| collection | DOAJ |
| description | Purpose To evaluate the results of a comparative analysis of the in vitro antimicrobial activity of polymer hydrogels and PMMA impregnated with
antibiotics against test cultures of the leading pathogens of bone infection. Materials and methods A comparative analysis of the antimicrobial activity
of the polymer hydrogel and PMMA impregnated with antibiotics was carried out. The bactericidal efficacy and prolongation of action of the following
microbe-antibiotic pairs were assessed: MSSA – gentamicin, MSSE – cefazolin, MRSA and MRSE – vancomycin, A. baumannii – tobramycin. The
duration of the study is 7 days. Statistical comparison of groups was carried out using the Mann-Whitney and Wilcoxon tests; the median and 95 % CI
were used to describe the data. Results The obtained indicators of the zone of inhibition of the growth of microorganisms around the polymer hydrogel
were several times higher than the results of PMMA (p < 0.05 for all periods). The antimicrobial activity of hydrogels in all cases lasted more than 7 days.
Hydrogels impregnated with vancomycin showed the lowest rate of contraction of the inhibition zone. The best antimicrobial effect was demonstrated
by the compared samples with cefazolin in relation to MSSE, the maximum index of the zone of suppression of which, on average, for the hydrogel was
29.3 mm, PMMA – 22.3 mm. Discussion Samples from polymer hydrogel impregnated with antibiotics demonstrated prolonged pronounced effective
antimicrobial activity against the leading pathogens of orthopedic infections in comparison with PMMA. The bactericidal efficacy of the polymer
hydrogel significantly exceeded the activity of PMMA already from the first day of the study (p = 0.002). The action of the hydrogel throughout the
study was uniform without abrupt changes or dips (p > 0.05), in contrast to bone cement, whose activity against all test cultures significantly decreased
(p = 0.042) on the 2nd day of the study. Conclusion Polymeric biodegradable hydrogels impregnated with antibiotics have a greater antimicrobial
potential compared to PMMA. Uniform, slow and controlled release of the drug and effective inhibition of the growth of microorganisms, as well as
prolonged action show that these properties may turn hydrogels into an effective depot system. |
| first_indexed | 2024-04-11T13:00:12Z |
| format | Article |
| id | doaj.art-3664a9bfe55940ff9a4c159b17952ebd |
| institution | Directory Open Access Journal |
| issn | 1028-4427 2542-131X |
| language | English |
| last_indexed | 2024-04-11T13:00:12Z |
| publishDate | 2022-12-01 |
| publisher | Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics |
| record_format | Article |
| series | Гений oртопедии |
| spelling | doaj.art-3664a9bfe55940ff9a4c159b17952ebd2022-12-22T04:22:56ZengRussian Ilizarov Scientific Center for Restorative Traumatology and OrthopaedicsГений oртопедии1028-44272542-131X2022-12-0128679480210.18019/1028-4427-2022-28-6-794-802In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infectionArchil V. Tsiskarashvili0Regina E. Melikova1Tamara Ya. Pkhakadze2Alexander A. Artyukhov3Natalya V. Sokorova4National Medical Research Center of Traumatology and Orthopedics n.a. N.N. Priorov, Moscow, Russian Federation; BIOORTHOTECH Research and Production Center, SKOLKOVO Innovation Center, Moscow, Russian FederationNational Medical Research Center of Traumatology and Orthopedics n.a. N.N. Priorov, Moscow, Russian FederationNational Medical Research Center of Traumatology and Orthopedics n.a. N.N. Priorov, Moscow, Russian FederationMendeleev University of Chemical Technology, Moscow, Russian FederationMendeleev University of Chemical Technology, Moscow, Russian FederationPurpose To evaluate the results of a comparative analysis of the in vitro antimicrobial activity of polymer hydrogels and PMMA impregnated with antibiotics against test cultures of the leading pathogens of bone infection. Materials and methods A comparative analysis of the antimicrobial activity of the polymer hydrogel and PMMA impregnated with antibiotics was carried out. The bactericidal efficacy and prolongation of action of the following microbe-antibiotic pairs were assessed: MSSA – gentamicin, MSSE – cefazolin, MRSA and MRSE – vancomycin, A. baumannii – tobramycin. The duration of the study is 7 days. Statistical comparison of groups was carried out using the Mann-Whitney and Wilcoxon tests; the median and 95 % CI were used to describe the data. Results The obtained indicators of the zone of inhibition of the growth of microorganisms around the polymer hydrogel were several times higher than the results of PMMA (p < 0.05 for all periods). The antimicrobial activity of hydrogels in all cases lasted more than 7 days. Hydrogels impregnated with vancomycin showed the lowest rate of contraction of the inhibition zone. The best antimicrobial effect was demonstrated by the compared samples with cefazolin in relation to MSSE, the maximum index of the zone of suppression of which, on average, for the hydrogel was 29.3 mm, PMMA – 22.3 mm. Discussion Samples from polymer hydrogel impregnated with antibiotics demonstrated prolonged pronounced effective antimicrobial activity against the leading pathogens of orthopedic infections in comparison with PMMA. The bactericidal efficacy of the polymer hydrogel significantly exceeded the activity of PMMA already from the first day of the study (p = 0.002). The action of the hydrogel throughout the study was uniform without abrupt changes or dips (p > 0.05), in contrast to bone cement, whose activity against all test cultures significantly decreased (p = 0.042) on the 2nd day of the study. Conclusion Polymeric biodegradable hydrogels impregnated with antibiotics have a greater antimicrobial potential compared to PMMA. Uniform, slow and controlled release of the drug and effective inhibition of the growth of microorganisms, as well as prolonged action show that these properties may turn hydrogels into an effective depot system.antimicrobial activitypolymeric biodegradable hydrogelbone cementorthopedic infectionin vitro studypolymethyl methacrylate (pmma) |
| spellingShingle | Archil V. Tsiskarashvili Regina E. Melikova Tamara Ya. Pkhakadze Alexander A. Artyukhov Natalya V. Sokorova In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infection Гений oртопедии antimicrobial activity polymeric biodegradable hydrogel bone cement orthopedic infection in vitro study polymethyl methacrylate (pmma) |
| title | In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infection |
| title_full | In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infection |
| title_fullStr | In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infection |
| title_full_unstemmed | In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infection |
| title_short | In vitro study of the antimicrobial activity of hydrogel-based matrices impregnated with antibiotics against the leading microorganisms of bone infection |
| title_sort | in vitro study of the antimicrobial activity of hydrogel based matrices impregnated with antibiotics against the leading microorganisms of bone infection |
| topic | antimicrobial activity polymeric biodegradable hydrogel bone cement orthopedic infection in vitro study polymethyl methacrylate (pmma) |
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