27 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight Analysis
OBJECTIVES/GOALS: We developed a non-invasive test of ocular alignment (OA) as a measure of otolith asymmetry, which impacts vestibular function. This test has also been administered on commercial astronauts to study the effect of microgravity on vestibular function. We will assess OA test performan...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2023-04-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866123001243/type/journal_article |
| _version_ | 1797840466487541760 |
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| author | Nabila Ali Johns Sabina Haberlen Phyllis Friello Kylie Perkins Mallika Sarma Mark Shelhamer |
| author_facet | Nabila Ali Johns Sabina Haberlen Phyllis Friello Kylie Perkins Mallika Sarma Mark Shelhamer |
| author_sort | Nabila Ali Johns |
| collection | DOAJ |
| description | OBJECTIVES/GOALS: We developed a non-invasive test of ocular alignment (OA) as a measure of otolith asymmetry, which impacts vestibular function. This test has also been administered on commercial astronauts to study the effect of microgravity on vestibular function. We will assess OA test performance in ground subjects, which has not yet been characterized. METHODS/STUDY POPULATION: Subjects were recruited from visitors to Space Center Houston from June to October 2022. Participants wore red-blue glasses in a darkened room and aligned two vertically or torsionally misaligned line segments, one red and one blue, on a tablet. Each subject underwent 11 vertical and torsional trials. The remaining misalignment was used to calculate ocular misalignment. Subjects self-reported demographic and health data. We calculated descriptive statistics and explored the distribution of overall vertical and torsional OA and distributions by age. Next steps will establish the reliability of the outcome measures with intraclass correlation coefficients and analyze associations between demographic variables and health status with OA performance using linear regression analysis. RESULTS/ANTICIPATED RESULTS: A total of 143 participants (67 female) with a median age of 21 (range 5-74 years old) completed OA testing. Of participants, 64% identified as white, 10% as black, 14% as Asian, 3% as Native American, and 8% as unknown. This test battery was feasible to implement in this mixed-age sample, with a 95% completion rate. Median absolute vertical OA was 0.08 degrees (IQR = 0.17 degrees), and median absolute torsional OA was 0.80 degrees (IQR = 1.20 degrees). Visual analysis of plots of OA by trial number reveals no apparent learning effect within subjects, though some individuals had considerable variation in performance. Average absolute vertical ocular misalignment increased modestly when plotted by age (fitted regression line b = 0.0032 degrees per year of age, p = 0.004), unadjusted for potential confounders. DISCUSSION/SIGNIFICANCE: Ocular alignment testing was feasible in a mixed-age general population sample. Our findings will expand understanding of how OA varies with age. These results will be used to create a normative database to compare and inform analysis of OA results from commercial spaceflights, which include individuals of varying age and health statuses. |
| first_indexed | 2024-04-09T16:15:33Z |
| format | Article |
| id | doaj.art-366662da9d14494aa1249a62fd4feb8d |
| institution | Directory Open Access Journal |
| issn | 2059-8661 |
| language | English |
| last_indexed | 2024-04-09T16:15:33Z |
| publishDate | 2023-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj.art-366662da9d14494aa1249a62fd4feb8d2023-04-24T05:55:55ZengCambridge University PressJournal of Clinical and Translational Science2059-86612023-04-0177710.1017/cts.2023.12427 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight AnalysisNabila Ali Johns0Sabina Haberlen1Phyllis Friello2Kylie Perkins3Mallika Sarma4Mark Shelhamer5Hopkins School of MedicineJohns Hopkins Bloomberg School of Public HealthSpace Center HoustonSpace Center HoustonDepartment of Otolaryngology, Johns Hopkins School of MedicineDepartment of Otolaryngology, Johns Hopkins School of MedicineOBJECTIVES/GOALS: We developed a non-invasive test of ocular alignment (OA) as a measure of otolith asymmetry, which impacts vestibular function. This test has also been administered on commercial astronauts to study the effect of microgravity on vestibular function. We will assess OA test performance in ground subjects, which has not yet been characterized. METHODS/STUDY POPULATION: Subjects were recruited from visitors to Space Center Houston from June to October 2022. Participants wore red-blue glasses in a darkened room and aligned two vertically or torsionally misaligned line segments, one red and one blue, on a tablet. Each subject underwent 11 vertical and torsional trials. The remaining misalignment was used to calculate ocular misalignment. Subjects self-reported demographic and health data. We calculated descriptive statistics and explored the distribution of overall vertical and torsional OA and distributions by age. Next steps will establish the reliability of the outcome measures with intraclass correlation coefficients and analyze associations between demographic variables and health status with OA performance using linear regression analysis. RESULTS/ANTICIPATED RESULTS: A total of 143 participants (67 female) with a median age of 21 (range 5-74 years old) completed OA testing. Of participants, 64% identified as white, 10% as black, 14% as Asian, 3% as Native American, and 8% as unknown. This test battery was feasible to implement in this mixed-age sample, with a 95% completion rate. Median absolute vertical OA was 0.08 degrees (IQR = 0.17 degrees), and median absolute torsional OA was 0.80 degrees (IQR = 1.20 degrees). Visual analysis of plots of OA by trial number reveals no apparent learning effect within subjects, though some individuals had considerable variation in performance. Average absolute vertical ocular misalignment increased modestly when plotted by age (fitted regression line b = 0.0032 degrees per year of age, p = 0.004), unadjusted for potential confounders. DISCUSSION/SIGNIFICANCE: Ocular alignment testing was feasible in a mixed-age general population sample. Our findings will expand understanding of how OA varies with age. These results will be used to create a normative database to compare and inform analysis of OA results from commercial spaceflights, which include individuals of varying age and health statuses.https://www.cambridge.org/core/product/identifier/S2059866123001243/type/journal_article |
| spellingShingle | Nabila Ali Johns Sabina Haberlen Phyllis Friello Kylie Perkins Mallika Sarma Mark Shelhamer 27 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight Analysis Journal of Clinical and Translational Science |
| title | 27 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight Analysis |
| title_full | 27 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight Analysis |
| title_fullStr | 27 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight Analysis |
| title_full_unstemmed | 27 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight Analysis |
| title_short | 27 Measuring Ocular Misalignment by Age at Space Center Houston to Inform Spaceflight Analysis |
| title_sort | 27 measuring ocular misalignment by age at space center houston to inform spaceflight analysis |
| url | https://www.cambridge.org/core/product/identifier/S2059866123001243/type/journal_article |
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