Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation
CONTEXT AND OBJECTIVE: Non-myeloablative hematopoietic stem cell transplantation (NMA-HSCT) is performed in onco-hematological patients who cannot tolerate ablative conditioning because of older age or comorbidities. This approach does not completely eliminate host cells and initially results in mix...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Paulista de Medicina
|
| Series: | São Paulo Medical Journal |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802009000500002&lng=en&tlng=en |
| _version_ | 1819021507214442496 |
|---|---|
| author | Azulamara da Silva Ruiz Maria de Lourdes Ferrari Chauffaille Solivanda Trindade Alves José Salvador Rodrigues de Oliveira |
| author_facet | Azulamara da Silva Ruiz Maria de Lourdes Ferrari Chauffaille Solivanda Trindade Alves José Salvador Rodrigues de Oliveira |
| author_sort | Azulamara da Silva Ruiz |
| collection | DOAJ |
| description | CONTEXT AND OBJECTIVE: Non-myeloablative hematopoietic stem cell transplantation (NMA-HSCT) is performed in onco-hematological patients who cannot tolerate ablative conditioning because of older age or comorbidities. This approach does not completely eliminate host cells and initially results in mixed chimerism. Long-term persistence of mixed chimerism results in graft rejection and relapse. Involvement of graft-versus-host disease is concomitant with complete chimerism and graft-versus-tumor effect. The aim of this study was to evaluate the prevalence of chimerism in onco-hematological patients who underwent NMA-HSCT. DESIGN AND SETTING: Observational clinical study on chimerism status after human leukocyte antigen-identical NMA-HSCT at the Discipline of Hematology and Hemotherapy of Universidade Federal de São Paulo. METHODS: We sequentially analyzed the amplification of APO-B, D1S80, DxS52, FVW, 33.6, YNZ-2 and H-ras primers using variable number of tandem repeats (VNTR) on 17 pairs and fluorescent in situ hybridization (FISH) with the XY probe and SRY primer on 13 sex-unmatched pairs. RESULTS: The informativeness of the primers using VNTR was 60% for APO-B, 75% D1S80, 36% DxS52, 14% FVW, 40% YNZ-22 and 16% H-ras. The SRY primer was informative in female receptors with male donors. The XY-FISH method was informative in 100% of the sex-unmatched pairs. CONCLUSION: These methods were sensitive and informative. In VNTR, the association of APO-B with D1S80 showed 88% informativeness. The quantitative FISH method was more sensitive, but had the disadvantage of only being used for sex-unmatched pairs. |
| first_indexed | 2024-12-21T04:08:12Z |
| format | Article |
| id | doaj.art-3667abd64c464079aff7c9ffda32ab8e |
| institution | Directory Open Access Journal |
| issn | 1806-9460 |
| language | English |
| last_indexed | 2024-12-21T04:08:12Z |
| publisher | Associação Paulista de Medicina |
| record_format | Article |
| series | São Paulo Medical Journal |
| spelling | doaj.art-3667abd64c464079aff7c9ffda32ab8e2022-12-21T19:16:33ZengAssociação Paulista de MedicinaSão Paulo Medical Journal1806-9460127525125810.1590/S1516-31802009000500002S1516-31802009000500002Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantationAzulamara da Silva Ruiz0Maria de Lourdes Ferrari Chauffaille1Solivanda Trindade Alves2José Salvador Rodrigues de Oliveira3Universidade Federal de São PauloUniversidade Federal de São PauloHospital Santa MarcelinaUniversidade Federal de São PauloCONTEXT AND OBJECTIVE: Non-myeloablative hematopoietic stem cell transplantation (NMA-HSCT) is performed in onco-hematological patients who cannot tolerate ablative conditioning because of older age or comorbidities. This approach does not completely eliminate host cells and initially results in mixed chimerism. Long-term persistence of mixed chimerism results in graft rejection and relapse. Involvement of graft-versus-host disease is concomitant with complete chimerism and graft-versus-tumor effect. The aim of this study was to evaluate the prevalence of chimerism in onco-hematological patients who underwent NMA-HSCT. DESIGN AND SETTING: Observational clinical study on chimerism status after human leukocyte antigen-identical NMA-HSCT at the Discipline of Hematology and Hemotherapy of Universidade Federal de São Paulo. METHODS: We sequentially analyzed the amplification of APO-B, D1S80, DxS52, FVW, 33.6, YNZ-2 and H-ras primers using variable number of tandem repeats (VNTR) on 17 pairs and fluorescent in situ hybridization (FISH) with the XY probe and SRY primer on 13 sex-unmatched pairs. RESULTS: The informativeness of the primers using VNTR was 60% for APO-B, 75% D1S80, 36% DxS52, 14% FVW, 40% YNZ-22 and 16% H-ras. The SRY primer was informative in female receptors with male donors. The XY-FISH method was informative in 100% of the sex-unmatched pairs. CONCLUSION: These methods were sensitive and informative. In VNTR, the association of APO-B with D1S80 showed 88% informativeness. The quantitative FISH method was more sensitive, but had the disadvantage of only being used for sex-unmatched pairs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802009000500002&lng=en&tlng=enVariable number of tandem repeatsHematologic neoplasmsBone marrow transplantationIn situ hybridization, fluorescenceChimerismLeukemia |
| spellingShingle | Azulamara da Silva Ruiz Maria de Lourdes Ferrari Chauffaille Solivanda Trindade Alves José Salvador Rodrigues de Oliveira Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation São Paulo Medical Journal Variable number of tandem repeats Hematologic neoplasms Bone marrow transplantation In situ hybridization, fluorescence Chimerism Leukemia |
| title | Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation |
| title_full | Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation |
| title_fullStr | Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation |
| title_full_unstemmed | Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation |
| title_short | Prevalence of chimerism after non-myeloablative hematopoietic stem cell transplantation |
| title_sort | prevalence of chimerism after non myeloablative hematopoietic stem cell transplantation |
| topic | Variable number of tandem repeats Hematologic neoplasms Bone marrow transplantation In situ hybridization, fluorescence Chimerism Leukemia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802009000500002&lng=en&tlng=en |
| work_keys_str_mv | AT azulamaradasilvaruiz prevalenceofchimerismafternonmyeloablativehematopoieticstemcelltransplantation AT mariadelourdesferrarichauffaille prevalenceofchimerismafternonmyeloablativehematopoieticstemcelltransplantation AT solivandatrindadealves prevalenceofchimerismafternonmyeloablativehematopoieticstemcelltransplantation AT josesalvadorrodriguesdeoliveira prevalenceofchimerismafternonmyeloablativehematopoieticstemcelltransplantation |