Molecular markers as tools in breeding for resistance to Potato Virus Y
Potato virus Y (PVY) is an economically important pathogen of potato as a vegetatively propagated crop. High resistance to all strains of PVY is determined by Ry-genes, which are introgressed into modern potato varieties from a limited number of sources of resistance ‒ Solanum stoloniferum Schlechtd...
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| Format: | Article |
| Language: | Russian |
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Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
2022-12-01
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| Series: | Аграрная наука Евро-Северо-Востока |
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| Online Access: | https://www.agronauka-sv.ru/jour/article/view/1170 |
| _version_ | 1797290188599197696 |
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| author | V. A. Biryukova V. A. Zharova N. A. Chalaya I. V. Shmyglya E. V. Rogozina |
| author_facet | V. A. Biryukova V. A. Zharova N. A. Chalaya I. V. Shmyglya E. V. Rogozina |
| author_sort | V. A. Biryukova |
| collection | DOAJ |
| description | Potato virus Y (PVY) is an economically important pathogen of potato as a vegetatively propagated crop. High resistance to all strains of PVY is determined by Ry-genes, which are introgressed into modern potato varieties from a limited number of sources of resistance ‒ Solanum stoloniferum Schlechtd. et Bche., Solanum andigenum Juz. et Buk., Solanum chacoense Bitt. Use of new species Solanum and interspecific hybrids based on them provides for the expansion of existing potato gene pool in breeding for resistance to PVY. Traditional breeding for resistance to viruses has still a great potential, though, it is a long and laborious process. Molecular markers linked to Ry-genes are widely integrated in order to increase the effectiveness of practical breeding. Previous studies have revealed a number of shortcomings in using the molecular markers of Ry-genes. To assess the predictive abilities of molecular markers RYSC3, M45, M6 of the Ryadg gene and YES3-3A of the Rysto gene for resistance to PVY, the F1 generation of two potato populations was studied, in the creation of which inter-specific hybrids were used. The nature of segregation 5:3 obtained by phenotype showed that the original parental forms can be the sources of not only previously identified, but also unidentified Ry-genes and Ny-genes of hypersensitivity. Correlation coefficient between the presence of markers and resistance to PVY was 0.64 for the YES3-3 marker (79 % matching) and 0.54 for RYSC3, М45, М6 markers (76 % matching). There have been revealed the cases of “false positive” results of the study (the presence of a marker in susceptible genotypes), which indicate to the insufficient effectiveness of the markers used. The marker segregation observed in the populations was consistent with chromatid segregation, confirming the simplex nature of Ry-genes inheritance from resistant parents. The ratio of genotypes with the presence/absence of markers was 0.86:1. |
| first_indexed | 2024-03-07T19:18:46Z |
| format | Article |
| id | doaj.art-366c252cadc146fba37cb1459d9807b7 |
| institution | Directory Open Access Journal |
| issn | 2072-9081 2500-1396 |
| language | Russian |
| last_indexed | 2024-03-07T19:18:46Z |
| publishDate | 2022-12-01 |
| publisher | Federal Agricultural Research Center of the North-East named N.V. Rudnitsky |
| record_format | Article |
| series | Аграрная наука Евро-Северо-Востока |
| spelling | doaj.art-366c252cadc146fba37cb1459d9807b72024-02-29T11:31:14ZrusFederal Agricultural Research Center of the North-East named N.V. RudnitskyАграрная наука Евро-Северо-Востока2072-90812500-13962022-12-0123677778710.30766/2072-9081.2022.23.6.777-787611Molecular markers as tools in breeding for resistance to Potato Virus YV. A. Biryukova0V. A. Zharova1N. A. Chalaya2I. V. Shmyglya3E. V. Rogozina4Russian Potato Research CentreRussian Potato Research CentreFederal Research Center the N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Russian Potato Research CentreFederal Research Center the N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Potato virus Y (PVY) is an economically important pathogen of potato as a vegetatively propagated crop. High resistance to all strains of PVY is determined by Ry-genes, which are introgressed into modern potato varieties from a limited number of sources of resistance ‒ Solanum stoloniferum Schlechtd. et Bche., Solanum andigenum Juz. et Buk., Solanum chacoense Bitt. Use of new species Solanum and interspecific hybrids based on them provides for the expansion of existing potato gene pool in breeding for resistance to PVY. Traditional breeding for resistance to viruses has still a great potential, though, it is a long and laborious process. Molecular markers linked to Ry-genes are widely integrated in order to increase the effectiveness of practical breeding. Previous studies have revealed a number of shortcomings in using the molecular markers of Ry-genes. To assess the predictive abilities of molecular markers RYSC3, M45, M6 of the Ryadg gene and YES3-3A of the Rysto gene for resistance to PVY, the F1 generation of two potato populations was studied, in the creation of which inter-specific hybrids were used. The nature of segregation 5:3 obtained by phenotype showed that the original parental forms can be the sources of not only previously identified, but also unidentified Ry-genes and Ny-genes of hypersensitivity. Correlation coefficient between the presence of markers and resistance to PVY was 0.64 for the YES3-3 marker (79 % matching) and 0.54 for RYSC3, М45, М6 markers (76 % matching). There have been revealed the cases of “false positive” results of the study (the presence of a marker in susceptible genotypes), which indicate to the insufficient effectiveness of the markers used. The marker segregation observed in the populations was consistent with chromatid segregation, confirming the simplex nature of Ry-genes inheritance from resistant parents. The ratio of genotypes with the presence/absence of markers was 0.86:1.https://www.agronauka-sv.ru/jour/article/view/1170<i>solanum tuberosum l.</i>resistance genesmarker-assistant selectionmolecular markers |
| spellingShingle | V. A. Biryukova V. A. Zharova N. A. Chalaya I. V. Shmyglya E. V. Rogozina Molecular markers as tools in breeding for resistance to Potato Virus Y Аграрная наука Евро-Северо-Востока <i>solanum tuberosum l.</i> resistance genes marker-assistant selection molecular markers |
| title | Molecular markers as tools in breeding for resistance to Potato Virus Y |
| title_full | Molecular markers as tools in breeding for resistance to Potato Virus Y |
| title_fullStr | Molecular markers as tools in breeding for resistance to Potato Virus Y |
| title_full_unstemmed | Molecular markers as tools in breeding for resistance to Potato Virus Y |
| title_short | Molecular markers as tools in breeding for resistance to Potato Virus Y |
| title_sort | molecular markers as tools in breeding for resistance to potato virus y |
| topic | <i>solanum tuberosum l.</i> resistance genes marker-assistant selection molecular markers |
| url | https://www.agronauka-sv.ru/jour/article/view/1170 |
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