The influence of C-ions and X-rays on human umbilical vein endothelial cells
Damage to the endothelium of blood vessels, which may occur during radiotherapy, is discussed as a potential precursor to the development of cardiovascular disease. We thus chose human umbilical vein endothelial cells (HUVEC) as a model system to examine the effect of low and high linear energy tran...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-01-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fonc.2016.00005/full |
| _version_ | 1811203753557098496 |
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| author | Alexander eHelm Ryonfa eLee Marco eDurante Marco eDurante Sylvia eRitter |
| author_facet | Alexander eHelm Ryonfa eLee Marco eDurante Marco eDurante Sylvia eRitter |
| author_sort | Alexander eHelm |
| collection | DOAJ |
| description | Damage to the endothelium of blood vessels, which may occur during radiotherapy, is discussed as a potential precursor to the development of cardiovascular disease. We thus chose human umbilical vein endothelial cells (HUVEC) as a model system to examine the effect of low and high linear energy transfer (LET) radiation. Cells were exposed to 250 kV X-rays or C-ions with the energies of either 9.8 MeV/u (LET=170 keV/µm) or 91 MeV/u (LET=28 keV/µm). Subculture of cells was performed regularly up to 49 days (~22 population doublings) post-irradiation. Immediately after exposure cells were seeded for the colony forming assay. Additionally, at regular intervals mitochondrial membrane potential (JC-1 staining) and cellular senescence (senescence associated β-galactosidase staining) were assessed. Cytogenetic damage was investigated by the micronucleus assay and the high-resolution mFISH technique. Analysis of radiation-induced damage shortly after exposure showed that C-ions are more effective than X-rays with respect to cell inactivation or the induction of cytogenetic damage (micronucleus assay) as observed in other cell systems. For 9.8 and 91 MeV/u C-ions relative biological effectiveness values of 2.4 and 1.5 were obtained for cell inactivation. At the subsequent time-points the number of micronucleated cells decreased to the control level. Analysis of chromosomal damage by mFISH technique revealed aberrations frequently involving chromosome 13 irrespective of dose or radiation quality. Disruption of the mitochondrial membrane potential was seen only a few days after exposure to X-rays or C-ions. Cellular senescence was not altered by radiation at any time-point investigated. Altogether our data indicate that C-ions were LET-dependently more effective in damaging endothelial cells shortly after exposure. Late damage to endothelial cells, however, was not found for the applied conditions and endpoints. |
| first_indexed | 2024-04-12T03:00:30Z |
| format | Article |
| id | doaj.art-366dacba3a124cc2adcb7e89eb5b9375 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T03:00:30Z |
| publishDate | 2016-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-366dacba3a124cc2adcb7e89eb5b93752022-12-22T03:50:40ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2016-01-01610.3389/fonc.2016.00005169875The influence of C-ions and X-rays on human umbilical vein endothelial cellsAlexander eHelm0Ryonfa eLee1Marco eDurante2Marco eDurante3Sylvia eRitter4GSI Helmholtz Centre for Heavy Ion ResearchGSI Helmholtz Centre for Heavy Ion ResearchGSI Helmholtz Centre for Heavy Ion ResearchTechnical University of DarmstadtGSI Helmholtz Centre for Heavy Ion ResearchDamage to the endothelium of blood vessels, which may occur during radiotherapy, is discussed as a potential precursor to the development of cardiovascular disease. We thus chose human umbilical vein endothelial cells (HUVEC) as a model system to examine the effect of low and high linear energy transfer (LET) radiation. Cells were exposed to 250 kV X-rays or C-ions with the energies of either 9.8 MeV/u (LET=170 keV/µm) or 91 MeV/u (LET=28 keV/µm). Subculture of cells was performed regularly up to 49 days (~22 population doublings) post-irradiation. Immediately after exposure cells were seeded for the colony forming assay. Additionally, at regular intervals mitochondrial membrane potential (JC-1 staining) and cellular senescence (senescence associated β-galactosidase staining) were assessed. Cytogenetic damage was investigated by the micronucleus assay and the high-resolution mFISH technique. Analysis of radiation-induced damage shortly after exposure showed that C-ions are more effective than X-rays with respect to cell inactivation or the induction of cytogenetic damage (micronucleus assay) as observed in other cell systems. For 9.8 and 91 MeV/u C-ions relative biological effectiveness values of 2.4 and 1.5 were obtained for cell inactivation. At the subsequent time-points the number of micronucleated cells decreased to the control level. Analysis of chromosomal damage by mFISH technique revealed aberrations frequently involving chromosome 13 irrespective of dose or radiation quality. Disruption of the mitochondrial membrane potential was seen only a few days after exposure to X-rays or C-ions. Cellular senescence was not altered by radiation at any time-point investigated. Altogether our data indicate that C-ions were LET-dependently more effective in damaging endothelial cells shortly after exposure. Late damage to endothelial cells, however, was not found for the applied conditions and endpoints.http://journal.frontiersin.org/Journal/10.3389/fonc.2016.00005/fullEndothelial Cellscardiovascular diseaseHigh LET RadiationCarbon ionsCarbon ion therapyChromosome 13 |
| spellingShingle | Alexander eHelm Ryonfa eLee Marco eDurante Marco eDurante Sylvia eRitter The influence of C-ions and X-rays on human umbilical vein endothelial cells Frontiers in Oncology Endothelial Cells cardiovascular disease High LET Radiation Carbon ions Carbon ion therapy Chromosome 13 |
| title | The influence of C-ions and X-rays on human umbilical vein endothelial cells |
| title_full | The influence of C-ions and X-rays on human umbilical vein endothelial cells |
| title_fullStr | The influence of C-ions and X-rays on human umbilical vein endothelial cells |
| title_full_unstemmed | The influence of C-ions and X-rays on human umbilical vein endothelial cells |
| title_short | The influence of C-ions and X-rays on human umbilical vein endothelial cells |
| title_sort | influence of c ions and x rays on human umbilical vein endothelial cells |
| topic | Endothelial Cells cardiovascular disease High LET Radiation Carbon ions Carbon ion therapy Chromosome 13 |
| url | http://journal.frontiersin.org/Journal/10.3389/fonc.2016.00005/full |
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