Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i>
Safer alternatives to synthetic pesticides are essential for sustainable agriculture. Methyl benzoate (MB) is a volatile essential oil found in several plants. Recent reports of the toxicity of MB to arthropod pests suggest that MB may be a useful alternative insecticide. The present study assessed...
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MDPI AG
2020-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/9/1313 |
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| author | Md Munir Mostafiz Md Badrul Alam Hsin Chi Errol Hassan Jae-Kyoung Shim Kyeong-Yeoll Lee |
| author_facet | Md Munir Mostafiz Md Badrul Alam Hsin Chi Errol Hassan Jae-Kyoung Shim Kyeong-Yeoll Lee |
| author_sort | Md Munir Mostafiz |
| collection | DOAJ |
| description | Safer alternatives to synthetic pesticides are essential for sustainable agriculture. Methyl benzoate (MB) is a volatile essential oil found in several plants. Recent reports of the toxicity of MB to arthropod pests suggest that MB may be a useful alternative insecticide. The present study assessed the effects of a sublethal concentration of MB (LC<sub>30</sub>, 0.22%) on the life history and reproductive characteristics of the cotton aphid, <i>Aphis gossypii</i> Glover, in both a treated parental generation (F<sub>0</sub>) and untreated progeny (F<sub>1</sub>). MB treatment significantly decreased longevity and fecundity in both the F<sub>0</sub> and F<sub>1</sub> generations, and prolonged the developmental duration of each immature instar of the F<sub>1</sub> generations, compared with controls. The intrinsic rate of increase (<i>r</i>), finite rate of increase (<i>λ</i>), and net reproductive rate (<i>R</i><sub>0</sub>) of the F<sub>1</sub> generation were significantly reduced, compared to controls. The mode of action of MB is not known, but in aphids treated with LC<sub>30</sub> MB, the activity of the enzyme acetylcholinesterase (AChE) decreased by more than 65%, compared with untreated controls. AChE activity was rapidly inhibited within 1 h, and remained inhibited for 6 h after in vivo exposure to MB. Moreover, molecular docking analysis revealed that MB had a strong affinity with the catalytic site of AChE, with a binding energy value of −6.2 kcal/mole. Our results suggest that MB targets AChE, and that a sublethal dose of MB can have adverse transgenerational effects on cotton aphids. |
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| language | English |
| last_indexed | 2024-03-10T16:36:38Z |
| publishDate | 2020-09-01 |
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| series | Agronomy |
| spelling | doaj.art-366eb752a5b94573a7f827cf6d1b9c442023-11-20T12:22:29ZengMDPI AGAgronomy2073-43952020-09-01109131310.3390/agronomy10091313Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i>Md Munir Mostafiz0Md Badrul Alam1Hsin Chi2Errol Hassan3Jae-Kyoung Shim4Kyeong-Yeoll Lee5Division of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, KoreaDepartment of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, KoreaDepartment of Entomology, National Chung Hsing University, Taichung 402, TaiwanSchool of Agriculture and Food Sciences, The University of Queensland Gatton, Gatton, QLD 4343, AustraliaDivision of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, KoreaDivision of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, KoreaSafer alternatives to synthetic pesticides are essential for sustainable agriculture. Methyl benzoate (MB) is a volatile essential oil found in several plants. Recent reports of the toxicity of MB to arthropod pests suggest that MB may be a useful alternative insecticide. The present study assessed the effects of a sublethal concentration of MB (LC<sub>30</sub>, 0.22%) on the life history and reproductive characteristics of the cotton aphid, <i>Aphis gossypii</i> Glover, in both a treated parental generation (F<sub>0</sub>) and untreated progeny (F<sub>1</sub>). MB treatment significantly decreased longevity and fecundity in both the F<sub>0</sub> and F<sub>1</sub> generations, and prolonged the developmental duration of each immature instar of the F<sub>1</sub> generations, compared with controls. The intrinsic rate of increase (<i>r</i>), finite rate of increase (<i>λ</i>), and net reproductive rate (<i>R</i><sub>0</sub>) of the F<sub>1</sub> generation were significantly reduced, compared to controls. The mode of action of MB is not known, but in aphids treated with LC<sub>30</sub> MB, the activity of the enzyme acetylcholinesterase (AChE) decreased by more than 65%, compared with untreated controls. AChE activity was rapidly inhibited within 1 h, and remained inhibited for 6 h after in vivo exposure to MB. Moreover, molecular docking analysis revealed that MB had a strong affinity with the catalytic site of AChE, with a binding energy value of −6.2 kcal/mole. Our results suggest that MB targets AChE, and that a sublethal dose of MB can have adverse transgenerational effects on cotton aphids.https://www.mdpi.com/2073-4395/10/9/1313natural pesticidesublethal effectstwo-sex life tablepopulation parametersenzyme activitymolecular target |
| spellingShingle | Md Munir Mostafiz Md Badrul Alam Hsin Chi Errol Hassan Jae-Kyoung Shim Kyeong-Yeoll Lee Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i> Agronomy natural pesticide sublethal effects two-sex life table population parameters enzyme activity molecular target |
| title | Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i> |
| title_full | Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i> |
| title_fullStr | Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i> |
| title_full_unstemmed | Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i> |
| title_short | Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of <i>Aphis gossypii</i> |
| title_sort | effects of sublethal doses of methyl benzoate on the life history traits and acetylcholinesterase ache activity of i aphis gossypii i |
| topic | natural pesticide sublethal effects two-sex life table population parameters enzyme activity molecular target |
| url | https://www.mdpi.com/2073-4395/10/9/1313 |
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