Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis
Deficiency in biosynthesis of inner core of lipopolysaccharide (LPS) rendered a characteristic biofilm-forming phenotype in E. coli. The pathological implications of this new phenotype in Shigella flexneri, a highly contagious enteric Gram-negative bacteria that is closely related to E. coli, were i...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2016-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/2178.pdf |
| _version_ | 1797422081638400000 |
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| author | Dan Xu Wei Zhang Bing Zhang Chongbing Liao Yongping Shao |
| author_facet | Dan Xu Wei Zhang Bing Zhang Chongbing Liao Yongping Shao |
| author_sort | Dan Xu |
| collection | DOAJ |
| description | Deficiency in biosynthesis of inner core of lipopolysaccharide (LPS) rendered a characteristic biofilm-forming phenotype in E. coli. The pathological implications of this new phenotype in Shigella flexneri, a highly contagious enteric Gram-negative bacteria that is closely related to E. coli, were investigated in this study. The ΔrfaC (also referred as waaC) mutant, with incomplete inner core of LPS due to deficiency in Hep biosynthesis, was characteristic of strong biofilm formation ability and exhibited much more pronounced adhesiveness and invasiveness to human epithelial cells than the parental strain and other LPS mutants, which also showed distinct pattern of F-actin recruitment. Failure to cause keratoconjunctivitis and colonize in the intestine in guinea pigs revealed that the fitness gain on host adhesion resulted from biofilm formation is not sufficient to offset the loss of fitness on survivability caused by LPS deletion. Our study suggests a clear positive relationship between increased surface hydrophobicity and adhesiveness of Shigella flexneri, which should be put into consideration of virulence of Shigella, especially when therapeutic strategy targeting the core oligosaccharide (OS) is considered an alternative to deal with bacterial antibiotics-resistance. |
| first_indexed | 2024-03-09T07:26:59Z |
| format | Article |
| id | doaj.art-3672e6f136ae480888174f686819dd7d |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:26:59Z |
| publishDate | 2016-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-3672e6f136ae480888174f686819dd7d2023-12-03T06:52:12ZengPeerJ Inc.PeerJ2167-83592016-07-014e217810.7717/peerj.2178Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesisDan Xu0Wei Zhang1Bing Zhang2Chongbing Liao3Yongping Shao4Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaKey Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaKey Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaKey Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaKey Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDeficiency in biosynthesis of inner core of lipopolysaccharide (LPS) rendered a characteristic biofilm-forming phenotype in E. coli. The pathological implications of this new phenotype in Shigella flexneri, a highly contagious enteric Gram-negative bacteria that is closely related to E. coli, were investigated in this study. The ΔrfaC (also referred as waaC) mutant, with incomplete inner core of LPS due to deficiency in Hep biosynthesis, was characteristic of strong biofilm formation ability and exhibited much more pronounced adhesiveness and invasiveness to human epithelial cells than the parental strain and other LPS mutants, which also showed distinct pattern of F-actin recruitment. Failure to cause keratoconjunctivitis and colonize in the intestine in guinea pigs revealed that the fitness gain on host adhesion resulted from biofilm formation is not sufficient to offset the loss of fitness on survivability caused by LPS deletion. Our study suggests a clear positive relationship between increased surface hydrophobicity and adhesiveness of Shigella flexneri, which should be put into consideration of virulence of Shigella, especially when therapeutic strategy targeting the core oligosaccharide (OS) is considered an alternative to deal with bacterial antibiotics-resistance.https://peerj.com/articles/2178.pdfShigella flexneriBiofilm formationEnhanced adhesion |
| spellingShingle | Dan Xu Wei Zhang Bing Zhang Chongbing Liao Yongping Shao Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis PeerJ Shigella flexneri Biofilm formation Enhanced adhesion |
| title | Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis |
| title_full | Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis |
| title_fullStr | Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis |
| title_full_unstemmed | Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis |
| title_short | Characterization of a biofilm-forming Shigella flexneri phenotype due to deficiency in Hep biosynthesis |
| title_sort | characterization of a biofilm forming shigella flexneri phenotype due to deficiency in hep biosynthesis |
| topic | Shigella flexneri Biofilm formation Enhanced adhesion |
| url | https://peerj.com/articles/2178.pdf |
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