Suppression of type III effector secretion by polymers
Bacteria secrete effector proteins required for successful infection and expression of toxicity into host cells. The type III secretion apparatus is involved in these processes. Previously, we showed that the viscous polymer polyethylene glycol (PEG) 8000 suppressed effector secretion by Pseudomonas...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2013-01-01
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| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.130133 |
| _version_ | 1818417835775361024 |
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| author | Takashi Ohgita Naoki Hayashi Naomasa Gotoh Kentaro Kogure |
| author_facet | Takashi Ohgita Naoki Hayashi Naomasa Gotoh Kentaro Kogure |
| author_sort | Takashi Ohgita |
| collection | DOAJ |
| description | Bacteria secrete effector proteins required for successful infection and expression of toxicity into host cells. The type III secretion apparatus is involved in these processes. Previously, we showed that the viscous polymer polyethylene glycol (PEG) 8000 suppressed effector secretion by Pseudomonas aeruginosa. We thus considered that other viscous polymers might also suppress secretion. We initially showed that PEG200 (formed from the same monomer (ethylene glycol) as PEG8000, but which forms solutions of lower viscosity than the latter compound) did not decrease effector secretion. By contrast, alginate, a high-viscous polymer formed from mannuronic and guluronic acid, unlike PEG8000, effectively inhibited secretion. The effectiveness of PEG8000 and alginate in this regard was closely associated with polymer viscosity, but the nature of viscosity dependence differed between the two polymers. Moreover, not only the natural polymer alginate, but also mucin, which protects against infection, suppressed secretion. We thus confirmed that polymer viscosity contributes to the suppression of effector secretion, but other factors (e.g. electrostatic interaction) may also be involved. Moreover, the results suggest that regulation of bacterial secretion by polymers may occur naturally via the action of components of biofilm or mucin layer. |
| first_indexed | 2024-12-14T12:13:06Z |
| format | Article |
| id | doaj.art-3674f18109864648b4c3cabd9fc526fe |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-12-14T12:13:06Z |
| publishDate | 2013-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-3674f18109864648b4c3cabd9fc526fe2022-12-21T23:01:41ZengThe Royal SocietyOpen Biology2046-24412013-01-0131210.1098/rsob.130133130133Suppression of type III effector secretion by polymersTakashi OhgitaNaoki HayashiNaomasa GotohKentaro KogureBacteria secrete effector proteins required for successful infection and expression of toxicity into host cells. The type III secretion apparatus is involved in these processes. Previously, we showed that the viscous polymer polyethylene glycol (PEG) 8000 suppressed effector secretion by Pseudomonas aeruginosa. We thus considered that other viscous polymers might also suppress secretion. We initially showed that PEG200 (formed from the same monomer (ethylene glycol) as PEG8000, but which forms solutions of lower viscosity than the latter compound) did not decrease effector secretion. By contrast, alginate, a high-viscous polymer formed from mannuronic and guluronic acid, unlike PEG8000, effectively inhibited secretion. The effectiveness of PEG8000 and alginate in this regard was closely associated with polymer viscosity, but the nature of viscosity dependence differed between the two polymers. Moreover, not only the natural polymer alginate, but also mucin, which protects against infection, suppressed secretion. We thus confirmed that polymer viscosity contributes to the suppression of effector secretion, but other factors (e.g. electrostatic interaction) may also be involved. Moreover, the results suggest that regulation of bacterial secretion by polymers may occur naturally via the action of components of biofilm or mucin layer.https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.130133type iii secretionpolymerpseudomonas aeruginosapegalginatemucin |
| spellingShingle | Takashi Ohgita Naoki Hayashi Naomasa Gotoh Kentaro Kogure Suppression of type III effector secretion by polymers Open Biology type iii secretion polymer pseudomonas aeruginosa peg alginate mucin |
| title | Suppression of type III effector secretion by polymers |
| title_full | Suppression of type III effector secretion by polymers |
| title_fullStr | Suppression of type III effector secretion by polymers |
| title_full_unstemmed | Suppression of type III effector secretion by polymers |
| title_short | Suppression of type III effector secretion by polymers |
| title_sort | suppression of type iii effector secretion by polymers |
| topic | type iii secretion polymer pseudomonas aeruginosa peg alginate mucin |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.130133 |
| work_keys_str_mv | AT takashiohgita suppressionoftypeiiieffectorsecretionbypolymers AT naokihayashi suppressionoftypeiiieffectorsecretionbypolymers AT naomasagotoh suppressionoftypeiiieffectorsecretionbypolymers AT kentarokogure suppressionoftypeiiieffectorsecretionbypolymers |