Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation
The aim of this investigation was to monitor metronidazole concentrations in the gingival crevicular fluid (GCF) collected from periodontal pockets of dogs after treatment with an experimental 15% metronidazole gel. Five dogs had periodontitis induced by cotton ligatures placed subgingivally and mai...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2008-06-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242008000200009 |
| _version_ | 1811245825861353472 |
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| author | Sandra Sato Maria José Vieira Fonseca José Orestes Del Ciampo José Roberto Jabor Vinícius Pedrazzi |
| author_facet | Sandra Sato Maria José Vieira Fonseca José Orestes Del Ciampo José Roberto Jabor Vinícius Pedrazzi |
| author_sort | Sandra Sato |
| collection | DOAJ |
| description | The aim of this investigation was to monitor metronidazole concentrations in the gingival crevicular fluid (GCF) collected from periodontal pockets of dogs after treatment with an experimental 15% metronidazole gel. Five dogs had periodontitis induced by cotton ligatures placed subgingivally and maintained for a 30-day period. After the induction period, only pockets with 4 mm or deeper received the gel. Each pocket was filled up to the gingival margin by means of a syringe with a blunt-end needle. GCF was collected in paper strips and quantified in an electronic device before and after 15 minutes, 1 h, 6 h, 24 h and 48 h of gel administration. The GCF samples were assayed for metronidazole content by means of a high performance liquid chromatography method. Concentrations of metronidazole in the GCF of the 5 dogs (mean ± SD, in µg/mL) were 0 ± 0 before gel application and 47,185.75 ± 24,874.35 after 15 minutes, 26,457.34 ± 25,516.91 after 1 h, 24.18 ± 23.11 after 6 h, 3.78 ± 3.45 after 24 h and 3.34 ± 5.54 after 48 h. A single administration of the 15% metronidazole gel released the drug in the GCF of dogs in levels several-fold higher than the minimum inhibitory concentration for some periodontopathogens grown in subgingival biofilms for up to one hour, but metronidazole could be detected in the GCF at least 48 hours after the gel application. |
| first_indexed | 2024-04-12T14:45:04Z |
| format | Article |
| id | doaj.art-367796b3ed2d41ce8562097360c5b004 |
| institution | Directory Open Access Journal |
| issn | 1806-8324 |
| language | English |
| last_indexed | 2024-04-12T14:45:04Z |
| publishDate | 2008-06-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-367796b3ed2d41ce8562097360c5b0042022-12-22T03:28:40ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1806-83242008-06-0122214515010.1590/S1806-83242008000200009Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluationSandra SatoMaria José Vieira FonsecaJosé Orestes Del CiampoJosé Roberto JaborVinícius PedrazziThe aim of this investigation was to monitor metronidazole concentrations in the gingival crevicular fluid (GCF) collected from periodontal pockets of dogs after treatment with an experimental 15% metronidazole gel. Five dogs had periodontitis induced by cotton ligatures placed subgingivally and maintained for a 30-day period. After the induction period, only pockets with 4 mm or deeper received the gel. Each pocket was filled up to the gingival margin by means of a syringe with a blunt-end needle. GCF was collected in paper strips and quantified in an electronic device before and after 15 minutes, 1 h, 6 h, 24 h and 48 h of gel administration. The GCF samples were assayed for metronidazole content by means of a high performance liquid chromatography method. Concentrations of metronidazole in the GCF of the 5 dogs (mean ± SD, in µg/mL) were 0 ± 0 before gel application and 47,185.75 ± 24,874.35 after 15 minutes, 26,457.34 ± 25,516.91 after 1 h, 24.18 ± 23.11 after 6 h, 3.78 ± 3.45 after 24 h and 3.34 ± 5.54 after 48 h. A single administration of the 15% metronidazole gel released the drug in the GCF of dogs in levels several-fold higher than the minimum inhibitory concentration for some periodontopathogens grown in subgingival biofilms for up to one hour, but metronidazole could be detected in the GCF at least 48 hours after the gel application.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242008000200009PeriodontitisMetronidazoleDrug delivery systems |
| spellingShingle | Sandra Sato Maria José Vieira Fonseca José Orestes Del Ciampo José Roberto Jabor Vinícius Pedrazzi Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation Brazilian Oral Research Periodontitis Metronidazole Drug delivery systems |
| title | Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation |
| title_full | Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation |
| title_fullStr | Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation |
| title_full_unstemmed | Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation |
| title_short | Metronidazole-containing gel for the treatment of periodontitis: an in vivo evaluation |
| title_sort | metronidazole containing gel for the treatment of periodontitis an in vivo evaluation |
| topic | Periodontitis Metronidazole Drug delivery systems |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242008000200009 |
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