Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitis
In this study, we examined whether the documented increase of plasma triglycerides in patients with generalized aggressive periodontitis (GAgP) is associated with changes in lipoprotein subclass distribution and/or LDL-associated platelet-activating factor acetylhydrolase (PAF-AH) activity. Lipoprot...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005-12-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520328649 |
| _version_ | 1823935393987297280 |
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| author | Miguel L. Rufail Harvey A. Schenkein Suzanne E. Barbour John G. Tew Rik van Antwerpen |
| author_facet | Miguel L. Rufail Harvey A. Schenkein Suzanne E. Barbour John G. Tew Rik van Antwerpen |
| author_sort | Miguel L. Rufail |
| collection | DOAJ |
| description | In this study, we examined whether the documented increase of plasma triglycerides in patients with generalized aggressive periodontitis (GAgP) is associated with changes in lipoprotein subclass distribution and/or LDL-associated platelet-activating factor acetylhydrolase (PAF-AH) activity. Lipoprotein subclasses were analyzed in whole plasma samples using nuclear magnetic resonance methods. Compared with subjects without periodontitis (NP subjects; n = 12), GAgP subjects (n = 12) had higher plasma levels of large, medium, and small VLDL (35.0 ± 6.7 vs. 63.1 ± 9.6 nmol/l; P = 0.025), higher levels of intermediate density lipoprotein (24.8 ± 11.6 vs. 87.2 ± 16.6 nmol/l; P = 0.006), lower levels of large LDL (448.3 ± 48.5 vs. 315.8 ± 59.4 nmol/l; P = 0.098), and higher levels of small LDL (488.2 ± 104.2 vs. 946.7 ± 151.6 nmol/l; P = 0.021). The average size of LDL from NP and GAgP subjects was 21.4 ± 0.2 and 20.6 ± 0.3 nm, respectively (P = 0.031). Compared with NP subjects, GAgP subjects had a greater number of circulating LDL particles (961.3 ± 105.3 vs. 1,349.0 ± 133.2 nmol/l; P = 0.032). Differences in the plasma levels of large, medium, and small HDL were not statistically significant. NP and GAgP subjects had similar plasma levels of total LDL-associated PAF-AH activity; however, LDL of GAgP subjects contained less PAF-AH activity per microgram of LDL protein (1,458.0 ± 171.0 and 865.2 ± 134 pmol/min/μg; P = 0.014).These results indicate that, in general, GAgP subjects have a more atherogenic lipoprotein profile and lower LDL-associated PAF-AH activity than NP subjects. These differences may help explain the increased risk of GAgP subjects for cardiovascular disease. |
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| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T22:36:52Z |
| publishDate | 2005-12-01 |
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| spelling | doaj.art-367bb53e700c4d33ac67e45e92592a4e2022-12-21T22:13:34ZengElsevierJournal of Lipid Research0022-22752005-12-01461227522760Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitisMiguel L. Rufail0Harvey A. Schenkein1Suzanne E. Barbour2John G. Tew3Rik van Antwerpen4Department of Biochemistry, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, VA 23298Clinical Research Center for Periodontal Diseases, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, VA 23298Department of Biochemistry, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, VA 23298Department of Microbiology and Immunology, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, VA 23298To whom correspondence should be addressed.; Department of Biochemistry, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, VA 23298In this study, we examined whether the documented increase of plasma triglycerides in patients with generalized aggressive periodontitis (GAgP) is associated with changes in lipoprotein subclass distribution and/or LDL-associated platelet-activating factor acetylhydrolase (PAF-AH) activity. Lipoprotein subclasses were analyzed in whole plasma samples using nuclear magnetic resonance methods. Compared with subjects without periodontitis (NP subjects; n = 12), GAgP subjects (n = 12) had higher plasma levels of large, medium, and small VLDL (35.0 ± 6.7 vs. 63.1 ± 9.6 nmol/l; P = 0.025), higher levels of intermediate density lipoprotein (24.8 ± 11.6 vs. 87.2 ± 16.6 nmol/l; P = 0.006), lower levels of large LDL (448.3 ± 48.5 vs. 315.8 ± 59.4 nmol/l; P = 0.098), and higher levels of small LDL (488.2 ± 104.2 vs. 946.7 ± 151.6 nmol/l; P = 0.021). The average size of LDL from NP and GAgP subjects was 21.4 ± 0.2 and 20.6 ± 0.3 nm, respectively (P = 0.031). Compared with NP subjects, GAgP subjects had a greater number of circulating LDL particles (961.3 ± 105.3 vs. 1,349.0 ± 133.2 nmol/l; P = 0.032). Differences in the plasma levels of large, medium, and small HDL were not statistically significant. NP and GAgP subjects had similar plasma levels of total LDL-associated PAF-AH activity; however, LDL of GAgP subjects contained less PAF-AH activity per microgram of LDL protein (1,458.0 ± 171.0 and 865.2 ± 134 pmol/min/μg; P = 0.014).These results indicate that, in general, GAgP subjects have a more atherogenic lipoprotein profile and lower LDL-associated PAF-AH activity than NP subjects. These differences may help explain the increased risk of GAgP subjects for cardiovascular disease.http://www.sciencedirect.com/science/article/pii/S0022227520328649low density lipoproteinpattern-B lipoprotein profilelipoprotein-associated phospholipase A2nuclear magnetic resonanceplatelet-activating factor acetylhydrolase |
| spellingShingle | Miguel L. Rufail Harvey A. Schenkein Suzanne E. Barbour John G. Tew Rik van Antwerpen Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitis Journal of Lipid Research low density lipoprotein pattern-B lipoprotein profile lipoprotein-associated phospholipase A2 nuclear magnetic resonance platelet-activating factor acetylhydrolase |
| title | Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitis |
| title_full | Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitis |
| title_fullStr | Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitis |
| title_full_unstemmed | Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitis |
| title_short | Altered lipoprotein subclass distribution and PAF-AH activity in subjects with generalized aggressive periodontitis |
| title_sort | altered lipoprotein subclass distribution and paf ah activity in subjects with generalized aggressive periodontitis |
| topic | low density lipoprotein pattern-B lipoprotein profile lipoprotein-associated phospholipase A2 nuclear magnetic resonance platelet-activating factor acetylhydrolase |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520328649 |
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