Natural spread of Citrus tristeza virus in lemon varieties
Natural spread of Citrus tristeza virus (CTV) was monitored in different lemon varieties derived from shoot tip grafted (STG) selections proven to be free of CTV before planting. The trial was planted in November, 2007, with a randomized four-block design with four replications. This experiment was...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Leonardo Daniel Ploper
2012-06-01
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| Series: | Revista Industrial y Agrícola de Tucumán |
| Online Access: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-30182012000100006 |
| _version_ | 1811310220245204992 |
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| author | Julia Figueroa Lucas Foguet Cecilia Escobar Ponce de León Florencia Palacios Gustavo Martos Beatriz Stein |
| author_facet | Julia Figueroa Lucas Foguet Cecilia Escobar Ponce de León Florencia Palacios Gustavo Martos Beatriz Stein |
| author_sort | Julia Figueroa |
| collection | DOAJ |
| description | Natural spread of Citrus tristeza virus (CTV) was monitored in different lemon varieties derived from shoot tip grafted (STG) selections proven to be free of CTV before planting. The trial was planted in November, 2007, with a randomized four-block design with four replications. This experiment was originally established to compare selected clones with their duplicates recovered by STG. The selected clones were originally nucellar clones kept in the germplasm bank in the field for 30 to 40 years, so the plants were naturally infected with CTV. The lemon varieties tested were two lines of both Frost Eureka and Limoneira 8A Lisbon and one line of Feminello Santa Teresa and Genoa EEAT. Plants were grafted on Poncirus trifoliata Flying Dragon, except for Eureka lemon, which was grafted on 79 AC [Citrus reshni x (C. paradisi x P. Trifoliata)]. Direct immunoprinting-ELISA with 3DF1+3CA5 monoclonal antibodies (Plant Print Diagnostics, Valencia) was used to determine the presence of CTV in field samples. Each sample was composed of four young shoots collected from the four sides of each tree. Two diagnoses were performed, one in September 2009 and another in September 2010. STG trees showed significant differences in CTV infection among them. The variety most infected with CTV was Limoneria 8 A and the least infected was Feminello Santa Teresa. Infected plants were found in a random distribution in the plot. All selected lemon trees were CTV positive. CTV spread will be monitored until 100% of all varieties show positive. |
| first_indexed | 2024-04-13T09:54:35Z |
| format | Article |
| id | doaj.art-368084845ad54ae9b66ff36c604ca032 |
| institution | Directory Open Access Journal |
| issn | 0370-5404 1851-3018 |
| language | English |
| last_indexed | 2024-04-13T09:54:35Z |
| publishDate | 2012-06-01 |
| publisher | Leonardo Daniel Ploper |
| record_format | Article |
| series | Revista Industrial y Agrícola de Tucumán |
| spelling | doaj.art-368084845ad54ae9b66ff36c604ca0322022-12-22T02:51:27ZengLeonardo Daniel PloperRevista Industrial y Agrícola de Tucumán0370-54041851-30182012-06-018914346Natural spread of Citrus tristeza virus in lemon varietiesJulia FigueroaLucas FoguetCecilia Escobar Ponce de LeónFlorencia PalaciosGustavo MartosBeatriz SteinNatural spread of Citrus tristeza virus (CTV) was monitored in different lemon varieties derived from shoot tip grafted (STG) selections proven to be free of CTV before planting. The trial was planted in November, 2007, with a randomized four-block design with four replications. This experiment was originally established to compare selected clones with their duplicates recovered by STG. The selected clones were originally nucellar clones kept in the germplasm bank in the field for 30 to 40 years, so the plants were naturally infected with CTV. The lemon varieties tested were two lines of both Frost Eureka and Limoneira 8A Lisbon and one line of Feminello Santa Teresa and Genoa EEAT. Plants were grafted on Poncirus trifoliata Flying Dragon, except for Eureka lemon, which was grafted on 79 AC [Citrus reshni x (C. paradisi x P. Trifoliata)]. Direct immunoprinting-ELISA with 3DF1+3CA5 monoclonal antibodies (Plant Print Diagnostics, Valencia) was used to determine the presence of CTV in field samples. Each sample was composed of four young shoots collected from the four sides of each tree. Two diagnoses were performed, one in September 2009 and another in September 2010. STG trees showed significant differences in CTV infection among them. The variety most infected with CTV was Limoneria 8 A and the least infected was Feminello Santa Teresa. Infected plants were found in a random distribution in the plot. All selected lemon trees were CTV positive. CTV spread will be monitored until 100% of all varieties show positive.http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-30182012000100006 |
| spellingShingle | Julia Figueroa Lucas Foguet Cecilia Escobar Ponce de León Florencia Palacios Gustavo Martos Beatriz Stein Natural spread of Citrus tristeza virus in lemon varieties Revista Industrial y Agrícola de Tucumán |
| title | Natural spread of Citrus tristeza virus in lemon varieties |
| title_full | Natural spread of Citrus tristeza virus in lemon varieties |
| title_fullStr | Natural spread of Citrus tristeza virus in lemon varieties |
| title_full_unstemmed | Natural spread of Citrus tristeza virus in lemon varieties |
| title_short | Natural spread of Citrus tristeza virus in lemon varieties |
| title_sort | natural spread of citrus tristeza virus in lemon varieties |
| url | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-30182012000100006 |
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