The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes
Background & aim: In vitro maturation of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant cysteamine and &beta...
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2015-11-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-573&slc_lang=en&sid=1 |
| _version_ | 1797998567442350080 |
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| author | A Dehghan Manshadi M Rostami Hosseinkhani M Nikseresht A Hedayatpoor M Akbartabar toori S Mehrabi R Mahmoudi |
| author_facet | A Dehghan Manshadi M Rostami Hosseinkhani M Nikseresht A Hedayatpoor M Akbartabar toori S Mehrabi R Mahmoudi |
| author_sort | A Dehghan Manshadi |
| collection | DOAJ |
| description | Background & aim: In vitro maturation of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant cysteamine and &beta-Mercaptoethanol on maturation and fertilization of immature oocytes.
Methods: in this experimental study, following 48 hrs injection of 7.5 IU PMSG to immature female mice, the germinal vesicle oocytes from ovaries were removed and transferred to TCM199 culture medium containing 50 ng /ml insulin-like growth factor-1 and 100 &mumol Cysteamine and &beta -Mercaptoethanol. After 24 hrs of culture, the oocytes of MII in IVF were fertilized and embryonic development to the two cells was studied under an inverted microscope. Data analysis was performed by using ANOVA and Post hoc Tukey test.
Results: The results showed that the rate of maturation, fertilization and 2-cell embryo formation in GV oocytes with cumulus cells in TCM199 medium containing insulin-like growth factor-1, Cysteamine and BME were 92.10, 93.30, 80.60% and in the GV oocytes without Cumulus cells were cultured in the same medium were 65.80, 64.00, 58.60% respectively which were statistically significant (P <0.001).
Conclusion: In the present study, the simultaneous combination of insulin-like growth factor-1, &beta-Mercaptoethanol and CYS increased maturation, fertilization and developmental rate to 2-cells stage with cumulus cells more than the oocyte without cumulus cells to a greater extent. This represented the need of adding supplemental growth factors and antioxidants to the medium and is associated with cumulus cells. |
| first_indexed | 2024-04-11T10:50:49Z |
| format | Article |
| id | doaj.art-3680ea35f2914d9e9a0dc0f2fec121b1 |
| institution | Directory Open Access Journal |
| issn | 1728-6506 1728-6514 |
| language | fas |
| last_indexed | 2024-04-11T10:50:49Z |
| publishDate | 2015-11-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-3680ea35f2914d9e9a0dc0f2fec121b12022-12-22T04:28:55ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142015-11-01208720731The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice OocytesA Dehghan Manshadi0M Rostami Hosseinkhani1M Nikseresht2A Hedayatpoor3M Akbartabar toori4S Mehrabi5R Mahmoudi6 1Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran 1Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran departmant anatomy University of Medical Sciences, Tehran, Iran 4Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran 2Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran Background & aim: In vitro maturation of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant cysteamine and &beta-Mercaptoethanol on maturation and fertilization of immature oocytes. Methods: in this experimental study, following 48 hrs injection of 7.5 IU PMSG to immature female mice, the germinal vesicle oocytes from ovaries were removed and transferred to TCM199 culture medium containing 50 ng /ml insulin-like growth factor-1 and 100 &mumol Cysteamine and &beta -Mercaptoethanol. After 24 hrs of culture, the oocytes of MII in IVF were fertilized and embryonic development to the two cells was studied under an inverted microscope. Data analysis was performed by using ANOVA and Post hoc Tukey test. Results: The results showed that the rate of maturation, fertilization and 2-cell embryo formation in GV oocytes with cumulus cells in TCM199 medium containing insulin-like growth factor-1, Cysteamine and BME were 92.10, 93.30, 80.60% and in the GV oocytes without Cumulus cells were cultured in the same medium were 65.80, 64.00, 58.60% respectively which were statistically significant (P <0.001). Conclusion: In the present study, the simultaneous combination of insulin-like growth factor-1, &beta-Mercaptoethanol and CYS increased maturation, fertilization and developmental rate to 2-cells stage with cumulus cells more than the oocyte without cumulus cells to a greater extent. This represented the need of adding supplemental growth factors and antioxidants to the medium and is associated with cumulus cells.http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-573&slc_lang=en&sid=1Germinal vesicle oocytes insulin-like growth factor 1 Cysteamine Beta-Mercaptoethanol Cumulus cell in vitro maturation |
| spellingShingle | A Dehghan Manshadi M Rostami Hosseinkhani M Nikseresht A Hedayatpoor M Akbartabar toori S Mehrabi R Mahmoudi The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes Armaghane Danesh Bimonthly Journal Germinal vesicle oocytes insulin-like growth factor 1 Cysteamine Beta-Mercaptoethanol Cumulus cell in vitro maturation |
| title | The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes |
| title_full | The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes |
| title_fullStr | The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes |
| title_full_unstemmed | The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes |
| title_short | The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes |
| title_sort | effect of insulin like growth factor 1 cysteamine and β mercaptoethanol on the in vitro maturation of immature mice oocytes |
| topic | Germinal vesicle oocytes insulin-like growth factor 1 Cysteamine Beta-Mercaptoethanol Cumulus cell in vitro maturation |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-573&slc_lang=en&sid=1 |
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