Site-specific analysis of N-glycans from different sheep prion strains.
Prion diseases are a group of neurodegenerative diseases affecting a wide range of mammalian species, including humans. During the course of the disease, the abnormally folded scrapie prion protein (PrPSc) accumulates in the central nervous system where it causes neurodegeneration. In prion disorder...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-02-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1009232 |
| _version_ | 1818667026877513728 |
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| author | Natali Nakić Thanh Hoa Tran Mislav Novokmet Olivier Andreoletti Gordan Lauc Giuseppe Legname |
| author_facet | Natali Nakić Thanh Hoa Tran Mislav Novokmet Olivier Andreoletti Gordan Lauc Giuseppe Legname |
| author_sort | Natali Nakić |
| collection | DOAJ |
| description | Prion diseases are a group of neurodegenerative diseases affecting a wide range of mammalian species, including humans. During the course of the disease, the abnormally folded scrapie prion protein (PrPSc) accumulates in the central nervous system where it causes neurodegeneration. In prion disorders, the diverse spectrum of illnesses exists because of the presence of different isoforms of PrPSc where they occupy distinct conformational states called strains. Strains are biochemically distinguished by a characteristic three-band immunoblot pattern, defined by differences in the occupancy of two glycosylation sites on the prion protein (PrP). Characterization of the exact N-glycan structures attached on either PrPC or PrPSc is lacking. Here we report the characterization and comparison of N-glycans from two different sheep prion strains. PrPSc from both strains was isolated from brain tissue and enzymatically digested with trypsin. By using liquid chromatography coupled to electrospray mass spectrometry, a site-specific analysis was performed. A total of 100 structures were detected on both glycosylation sites. The N-glycan profile was shown to be similar to the one on mouse PrP, however, with additional 40 structures reported. The results presented here show no major differences in glycan composition, suggesting that glycans may not be responsible for the differences in the two analyzed prion strains. |
| first_indexed | 2024-12-17T06:13:53Z |
| format | Article |
| id | doaj.art-36823cb87fb44a3697ca22cbd80b079f |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-17T06:13:53Z |
| publishDate | 2021-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-36823cb87fb44a3697ca22cbd80b079f2022-12-21T22:00:34ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742021-02-01172e100923210.1371/journal.ppat.1009232Site-specific analysis of N-glycans from different sheep prion strains.Natali NakićThanh Hoa TranMislav NovokmetOlivier AndreolettiGordan LaucGiuseppe LegnamePrion diseases are a group of neurodegenerative diseases affecting a wide range of mammalian species, including humans. During the course of the disease, the abnormally folded scrapie prion protein (PrPSc) accumulates in the central nervous system where it causes neurodegeneration. In prion disorders, the diverse spectrum of illnesses exists because of the presence of different isoforms of PrPSc where they occupy distinct conformational states called strains. Strains are biochemically distinguished by a characteristic three-band immunoblot pattern, defined by differences in the occupancy of two glycosylation sites on the prion protein (PrP). Characterization of the exact N-glycan structures attached on either PrPC or PrPSc is lacking. Here we report the characterization and comparison of N-glycans from two different sheep prion strains. PrPSc from both strains was isolated from brain tissue and enzymatically digested with trypsin. By using liquid chromatography coupled to electrospray mass spectrometry, a site-specific analysis was performed. A total of 100 structures were detected on both glycosylation sites. The N-glycan profile was shown to be similar to the one on mouse PrP, however, with additional 40 structures reported. The results presented here show no major differences in glycan composition, suggesting that glycans may not be responsible for the differences in the two analyzed prion strains.https://doi.org/10.1371/journal.ppat.1009232 |
| spellingShingle | Natali Nakić Thanh Hoa Tran Mislav Novokmet Olivier Andreoletti Gordan Lauc Giuseppe Legname Site-specific analysis of N-glycans from different sheep prion strains. PLoS Pathogens |
| title | Site-specific analysis of N-glycans from different sheep prion strains. |
| title_full | Site-specific analysis of N-glycans from different sheep prion strains. |
| title_fullStr | Site-specific analysis of N-glycans from different sheep prion strains. |
| title_full_unstemmed | Site-specific analysis of N-glycans from different sheep prion strains. |
| title_short | Site-specific analysis of N-glycans from different sheep prion strains. |
| title_sort | site specific analysis of n glycans from different sheep prion strains |
| url | https://doi.org/10.1371/journal.ppat.1009232 |
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