Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilis
Autolysins are endogenous cell wall degrading enzymes (CWDEs) in bacteria that remodel the peptidoglycan layer of its own cell wall. In the Bacillus subtilis genome, at least 35 autolysin genes have been identified. However, the study of their roles in bacterial physiology has been hampered by their...
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| Format: | Article |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1381018/full |
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| author | Luotao Wang Jianquan Huang Si Chen Xin Su Xun Zhang Lujun Wang Wei Zhang Zhenshuo Wang Qingchao Zeng Qi Wang Yan Li |
| author_facet | Luotao Wang Jianquan Huang Si Chen Xin Su Xun Zhang Lujun Wang Wei Zhang Zhenshuo Wang Qingchao Zeng Qi Wang Yan Li |
| author_sort | Luotao Wang |
| collection | DOAJ |
| description | Autolysins are endogenous cell wall degrading enzymes (CWDEs) in bacteria that remodel the peptidoglycan layer of its own cell wall. In the Bacillus subtilis genome, at least 35 autolysin genes have been identified. However, the study of their roles in bacterial physiology has been hampered by their complexity and functional redundancy. B. subtilis GLB191 is an effective biocontrol strain against grape downy mildew disease, the biocontrol effect of which results from both direct effect against the pathogen and stimulation of the plant defense. In this study, we show that the autolysin N-acetylglucosaminidase LytD, a major autolysin of vegetative growth in B. subtilis, plays an important role in its biocontrol activity against grape downy mildew. Disruption of lytD resulted in reduced suppression of the pathogen Plasmopara viticola and stimulation of the plant defense. LytD is also shown to affect the biofilm formation and colonization of B. subtilis on grape leaves. This is the first report that demonstrates the role of an endogenous CWDE in suppressing plant disease infection of a biological control microorganism. These findings not only expand our knowledge on the biological function of autolysins but also provide a new target to promote the biocontrol activity of B. subtilis. |
| first_indexed | 2024-04-24T11:39:13Z |
| format | Article |
| id | doaj.art-368afd34f4584d5fa6c6b22aca1a2bcf |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-24T11:39:13Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-368afd34f4584d5fa6c6b22aca1a2bcf2024-04-10T04:26:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-04-011510.3389/fpls.2024.13810181381018Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilisLuotao Wang0Jianquan Huang1Si Chen2Xin Su3Xun Zhang4Lujun Wang5Wei Zhang6Zhenshuo Wang7Qingchao Zeng8Qi Wang9Yan Li10Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaThe Research Institute of Forestry and Pomology, Tianjin Academy of Agricultural Sciences, Tianjin, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaAirport Research Institute, China Academy of Civil Aviation Science and Technology, Beijing, ChinaWeinan Grapevine Research Institute, Weinan, ChinaWeinan Grapevine Research Institute, Weinan, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, ChinaAutolysins are endogenous cell wall degrading enzymes (CWDEs) in bacteria that remodel the peptidoglycan layer of its own cell wall. In the Bacillus subtilis genome, at least 35 autolysin genes have been identified. However, the study of their roles in bacterial physiology has been hampered by their complexity and functional redundancy. B. subtilis GLB191 is an effective biocontrol strain against grape downy mildew disease, the biocontrol effect of which results from both direct effect against the pathogen and stimulation of the plant defense. In this study, we show that the autolysin N-acetylglucosaminidase LytD, a major autolysin of vegetative growth in B. subtilis, plays an important role in its biocontrol activity against grape downy mildew. Disruption of lytD resulted in reduced suppression of the pathogen Plasmopara viticola and stimulation of the plant defense. LytD is also shown to affect the biofilm formation and colonization of B. subtilis on grape leaves. This is the first report that demonstrates the role of an endogenous CWDE in suppressing plant disease infection of a biological control microorganism. These findings not only expand our knowledge on the biological function of autolysins but also provide a new target to promote the biocontrol activity of B. subtilis.https://www.frontiersin.org/articles/10.3389/fpls.2024.1381018/fullautolysinpeptidoglycancell wall degrading enzymecolonizationbiofilminduced resistance |
| spellingShingle | Luotao Wang Jianquan Huang Si Chen Xin Su Xun Zhang Lujun Wang Wei Zhang Zhenshuo Wang Qingchao Zeng Qi Wang Yan Li Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilis Frontiers in Plant Science autolysin peptidoglycan cell wall degrading enzyme colonization biofilm induced resistance |
| title | Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilis |
| title_full | Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilis |
| title_fullStr | Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilis |
| title_full_unstemmed | Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilis |
| title_short | Endogenous cell wall degrading enzyme LytD is important for the biocontrol activity of Bacillus subtilis |
| title_sort | endogenous cell wall degrading enzyme lytd is important for the biocontrol activity of bacillus subtilis |
| topic | autolysin peptidoglycan cell wall degrading enzyme colonization biofilm induced resistance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1381018/full |
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