Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach
In the growing ovarian follicle, the maturing oocyte is accompanied by cumulus (CCs) and granulosa (GCs) cells. Currently, there remain many unanswered questions about the epithelial origin of these cells. Global and targeted gene transcript levels were assessed on 1, 7, 15, 30 days of culture for C...
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MDPI AG
2021-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/1/73 |
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| author | Błażej Chermuła Wiesława Kranc Piotr Celichowski Bogusława Stelmach Hanna Piotrowska-Kempisty Paul Mozdziak Leszek Pawelczyk Robert Zygmunt Spaczyński Bartosz Kempisty |
| author_facet | Błażej Chermuła Wiesława Kranc Piotr Celichowski Bogusława Stelmach Hanna Piotrowska-Kempisty Paul Mozdziak Leszek Pawelczyk Robert Zygmunt Spaczyński Bartosz Kempisty |
| author_sort | Błażej Chermuła |
| collection | DOAJ |
| description | In the growing ovarian follicle, the maturing oocyte is accompanied by cumulus (CCs) and granulosa (GCs) cells. Currently, there remain many unanswered questions about the epithelial origin of these cells. Global and targeted gene transcript levels were assessed on 1, 7, 15, 30 days of culture for CCs and GCs. Detailed analysis of the genes belonging to epithelial cell-associated ontological groups allowed us to assess a total of 168 genes expressed in CCs (97 genes) and GCs (71 genes) during long-term in vitro culture. Expression changes of the analyzed genes allowed the identification of the group of genes: TGFBR3, PTGS2, PRKX, AHI1, and IL11, whose expression decreased the most and the group of ANXA3, DKK1, CCND1, STC1, CAV1, and SFRP4 genes, whose expression significantly increased. These genes’ expression indicates CCs and GCs epithelialization processes and their epithelial origin. Expression change analysis of genes involved in epithelization processes in GCs and CCs during their in vitro culture made it possible to describe the most significantly altered of the 11 genes. Detailed analysis of gene expression in these two cell populations at different time intervals confirms their ovarian surface epithelial origin. Furthermore, some gene expression profiles appear to have tumorigenic properties, suggesting that granulosa cells may play a role in cancerogenesis. |
| first_indexed | 2024-03-10T03:36:29Z |
| format | Article |
| id | doaj.art-368c82aec15b4c23a601e5926a3e3214 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T03:36:29Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-368c82aec15b4c23a601e5926a3e32142023-11-23T11:43:14ZengMDPI AGJournal of Clinical Medicine2077-03832021-12-011117310.3390/jcm11010073Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical ApproachBłażej Chermuła0Wiesława Kranc1Piotr Celichowski2Bogusława Stelmach3Hanna Piotrowska-Kempisty4Paul Mozdziak5Leszek Pawelczyk6Robert Zygmunt Spaczyński7Bartosz Kempisty8Department of Gynecology, Division of Infertility and Reproductive Endocrinology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, PolandDepartment of Anatomy, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, PolandDepartment of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, PolandDepartment of Gynecology, Division of Infertility and Reproductive Endocrinology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznan, PolandPhysiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USADepartment of Gynecology, Division of Infertility and Reproductive Endocrinology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, PolandDepartment of Gynecology, Division of Infertility and Reproductive Endocrinology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 33 Polna St., 60-535 Poznan, PolandDepartment of Anatomy, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781 Poznan, PolandIn the growing ovarian follicle, the maturing oocyte is accompanied by cumulus (CCs) and granulosa (GCs) cells. Currently, there remain many unanswered questions about the epithelial origin of these cells. Global and targeted gene transcript levels were assessed on 1, 7, 15, 30 days of culture for CCs and GCs. Detailed analysis of the genes belonging to epithelial cell-associated ontological groups allowed us to assess a total of 168 genes expressed in CCs (97 genes) and GCs (71 genes) during long-term in vitro culture. Expression changes of the analyzed genes allowed the identification of the group of genes: TGFBR3, PTGS2, PRKX, AHI1, and IL11, whose expression decreased the most and the group of ANXA3, DKK1, CCND1, STC1, CAV1, and SFRP4 genes, whose expression significantly increased. These genes’ expression indicates CCs and GCs epithelialization processes and their epithelial origin. Expression change analysis of genes involved in epithelization processes in GCs and CCs during their in vitro culture made it possible to describe the most significantly altered of the 11 genes. Detailed analysis of gene expression in these two cell populations at different time intervals confirms their ovarian surface epithelial origin. Furthermore, some gene expression profiles appear to have tumorigenic properties, suggesting that granulosa cells may play a role in cancerogenesis.https://www.mdpi.com/2077-0383/11/1/73cumulus cellsgranulosa cellshumangene expressionmicroarrayepithelialization |
| spellingShingle | Błażej Chermuła Wiesława Kranc Piotr Celichowski Bogusława Stelmach Hanna Piotrowska-Kempisty Paul Mozdziak Leszek Pawelczyk Robert Zygmunt Spaczyński Bartosz Kempisty Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach Journal of Clinical Medicine cumulus cells granulosa cells human gene expression microarray epithelialization |
| title | Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach |
| title_full | Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach |
| title_fullStr | Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach |
| title_full_unstemmed | Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach |
| title_short | Cellular Processes in Human Ovarian Follicles Are Regulated by Expression Profile of New Gene Markers—Clinical Approach |
| title_sort | cellular processes in human ovarian follicles are regulated by expression profile of new gene markers clinical approach |
| topic | cumulus cells granulosa cells human gene expression microarray epithelialization |
| url | https://www.mdpi.com/2077-0383/11/1/73 |
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