Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences
Australasian marsupials harbour a diverse group of gastrointestinal strongyloid nematodes. These nematodes are currently grouped into two subfamilies, namely the Cloacininae and Phascolostrongylinae. Based on morphological criteria, the Cloacininae and Phascolostrongylinae were defined as monophylet...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/12/21/2900 |
| _version_ | 1797469398614671360 |
|---|---|
| author | Tanapan Sukee Ian Beveridge Anson V. Koehler Ross S. Hall Robin B. Gasser Abdul Jabbar |
| author_facet | Tanapan Sukee Ian Beveridge Anson V. Koehler Ross S. Hall Robin B. Gasser Abdul Jabbar |
| author_sort | Tanapan Sukee |
| collection | DOAJ |
| description | Australasian marsupials harbour a diverse group of gastrointestinal strongyloid nematodes. These nematodes are currently grouped into two subfamilies, namely the Cloacininae and Phascolostrongylinae. Based on morphological criteria, the Cloacininae and Phascolostrongylinae were defined as monophyletic and placed in the family Cloacinidae, but this has not been supported by molecular data and they are currently placed in the Chabertiidae. Although molecular data (internal transcribed spacers of the nuclear ribosomal RNA genes or mitochondrial protein-coding genes) have been used to verify morphological classifications within the Cloacininae and Phascolostrongylinae, the phylogenetic relationships between the subfamilies have not been rigorously tested. This study determined the phylogenetic relationships of the subfamilies Cloacininae and Phascolostrongylinae using amino acid sequences conceptually translated from the twelve concatenated mitochondrial protein-coding genes. The findings demonstrated that the Cloacininae and Phascolostrongylinae formed a well-supported monophyletic assemblage, consistent with their morphological classification as an independent family, Cloacinidae. Unexpectedly, however, the subfamily Phascolostrongylinae was split into two groups comprising the genera from macropodid hosts (kangaroos and wallabies) and those from vombatid hosts (wombats). Genera of the Cloacininae and Phascolostrongylinae occurring in macropodid hosts were more closely related compared to genera of the Phascolostrongylinae occurring in wombats that formed a sister relationship with the remaining genera from macropods. These findings provide molecular evidence supporting the monophyly of the family Cloacinidae and an alternative hypothesis for the origin of marsupial strongyloid nematodes in vombatid hosts that requires further exploration using molecular approaches and additional samples |
| first_indexed | 2024-03-09T19:20:52Z |
| format | Article |
| id | doaj.art-368db3aa38f7492191f9bc09df7c4766 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T19:20:52Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-368db3aa38f7492191f9bc09df7c47662023-11-24T03:23:27ZengMDPI AGAnimals2076-26152022-10-011221290010.3390/ani12212900Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein SequencesTanapan Sukee0Ian Beveridge1Anson V. Koehler2Ross S. Hall3Robin B. Gasser4Abdul Jabbar5Department of Veterinary Biosciences, School of Veterinary Science, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, VIC 3030, AustraliaDepartment of Veterinary Biosciences, School of Veterinary Science, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, VIC 3030, AustraliaDepartment of Veterinary Biosciences, School of Veterinary Science, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, VIC 3030, AustraliaDepartment of Veterinary Biosciences, School of Veterinary Science, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, VIC 3030, AustraliaDepartment of Veterinary Biosciences, School of Veterinary Science, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, VIC 3030, AustraliaDepartment of Veterinary Biosciences, School of Veterinary Science, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Werribee, VIC 3030, AustraliaAustralasian marsupials harbour a diverse group of gastrointestinal strongyloid nematodes. These nematodes are currently grouped into two subfamilies, namely the Cloacininae and Phascolostrongylinae. Based on morphological criteria, the Cloacininae and Phascolostrongylinae were defined as monophyletic and placed in the family Cloacinidae, but this has not been supported by molecular data and they are currently placed in the Chabertiidae. Although molecular data (internal transcribed spacers of the nuclear ribosomal RNA genes or mitochondrial protein-coding genes) have been used to verify morphological classifications within the Cloacininae and Phascolostrongylinae, the phylogenetic relationships between the subfamilies have not been rigorously tested. This study determined the phylogenetic relationships of the subfamilies Cloacininae and Phascolostrongylinae using amino acid sequences conceptually translated from the twelve concatenated mitochondrial protein-coding genes. The findings demonstrated that the Cloacininae and Phascolostrongylinae formed a well-supported monophyletic assemblage, consistent with their morphological classification as an independent family, Cloacinidae. Unexpectedly, however, the subfamily Phascolostrongylinae was split into two groups comprising the genera from macropodid hosts (kangaroos and wallabies) and those from vombatid hosts (wombats). Genera of the Cloacininae and Phascolostrongylinae occurring in macropodid hosts were more closely related compared to genera of the Phascolostrongylinae occurring in wombats that formed a sister relationship with the remaining genera from macropods. These findings provide molecular evidence supporting the monophyly of the family Cloacinidae and an alternative hypothesis for the origin of marsupial strongyloid nematodes in vombatid hosts that requires further exploration using molecular approaches and additional sampleshttps://www.mdpi.com/2076-2615/12/21/2900CloacininaePhascolostrongylinaeStrongyloideamitochondrial protein-coding genesphylogenetics |
| spellingShingle | Tanapan Sukee Ian Beveridge Anson V. Koehler Ross S. Hall Robin B. Gasser Abdul Jabbar Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences Animals Cloacininae Phascolostrongylinae Strongyloidea mitochondrial protein-coding genes phylogenetics |
| title | Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences |
| title_full | Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences |
| title_fullStr | Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences |
| title_full_unstemmed | Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences |
| title_short | Phylogenetic Relationships of the Strongyloid Nematodes of Australasian Marsupials Based on Mitochondrial Protein Sequences |
| title_sort | phylogenetic relationships of the strongyloid nematodes of australasian marsupials based on mitochondrial protein sequences |
| topic | Cloacininae Phascolostrongylinae Strongyloidea mitochondrial protein-coding genes phylogenetics |
| url | https://www.mdpi.com/2076-2615/12/21/2900 |
| work_keys_str_mv | AT tanapansukee phylogeneticrelationshipsofthestrongyloidnematodesofaustralasianmarsupialsbasedonmitochondrialproteinsequences AT ianbeveridge phylogeneticrelationshipsofthestrongyloidnematodesofaustralasianmarsupialsbasedonmitochondrialproteinsequences AT ansonvkoehler phylogeneticrelationshipsofthestrongyloidnematodesofaustralasianmarsupialsbasedonmitochondrialproteinsequences AT rossshall phylogeneticrelationshipsofthestrongyloidnematodesofaustralasianmarsupialsbasedonmitochondrialproteinsequences AT robinbgasser phylogeneticrelationshipsofthestrongyloidnematodesofaustralasianmarsupialsbasedonmitochondrialproteinsequences AT abduljabbar phylogeneticrelationshipsofthestrongyloidnematodesofaustralasianmarsupialsbasedonmitochondrialproteinsequences |