NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells.
Reactive oxygen species (ROS) primarily produced via NADPH oxidase play an important role for killing microorganisms in neutrophils. In this study we examined if ROS production in Human promyelocytic leukemia cells (HL60) differentiated into neutrophil-like cells (dHL60) induces ER stress and activa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4323339?pdf=render |
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| author | Wilson Mitsuo Tatagiba Kuwabara Liling Zhang Irmgard Schuiki Rui Curi Allen Volchuk Tatiana Carolina Alba-Loureiro |
| author_facet | Wilson Mitsuo Tatagiba Kuwabara Liling Zhang Irmgard Schuiki Rui Curi Allen Volchuk Tatiana Carolina Alba-Loureiro |
| author_sort | Wilson Mitsuo Tatagiba Kuwabara |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) primarily produced via NADPH oxidase play an important role for killing microorganisms in neutrophils. In this study we examined if ROS production in Human promyelocytic leukemia cells (HL60) differentiated into neutrophil-like cells (dHL60) induces ER stress and activates the unfolded protein response (UPR). To cause ROS production cells were treated with PMA or by chronic hyperglycemia. Chronic hyperglycemia failed to induce ROS production and did not cause activation of the UPR in dHL60 cells. PMA, a pharmacologic NADPH oxidase activator, induced ER stress in dHL60 cells as monitored by IRE-1 and PERK pathway activation, and this was independent of calcium signaling. The NADPH oxidase inhibitor, DPI, abolished both ROS production and UPR activation. These results show that ROS produced by NADPH oxidase induces ER stress and suggests a close association between the redox state of the cell and the activation of the UPR in neutrophil-like HL60 cells. |
| first_indexed | 2024-12-14T16:17:22Z |
| format | Article |
| id | doaj.art-369114e0e8584d3f9431d267b4e9a9eb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T16:17:22Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-369114e0e8584d3f9431d267b4e9a9eb2022-12-21T22:54:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011641010.1371/journal.pone.0116410NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells.Wilson Mitsuo Tatagiba KuwabaraLiling ZhangIrmgard SchuikiRui CuriAllen VolchukTatiana Carolina Alba-LoureiroReactive oxygen species (ROS) primarily produced via NADPH oxidase play an important role for killing microorganisms in neutrophils. In this study we examined if ROS production in Human promyelocytic leukemia cells (HL60) differentiated into neutrophil-like cells (dHL60) induces ER stress and activates the unfolded protein response (UPR). To cause ROS production cells were treated with PMA or by chronic hyperglycemia. Chronic hyperglycemia failed to induce ROS production and did not cause activation of the UPR in dHL60 cells. PMA, a pharmacologic NADPH oxidase activator, induced ER stress in dHL60 cells as monitored by IRE-1 and PERK pathway activation, and this was independent of calcium signaling. The NADPH oxidase inhibitor, DPI, abolished both ROS production and UPR activation. These results show that ROS produced by NADPH oxidase induces ER stress and suggests a close association between the redox state of the cell and the activation of the UPR in neutrophil-like HL60 cells.http://europepmc.org/articles/PMC4323339?pdf=render |
| spellingShingle | Wilson Mitsuo Tatagiba Kuwabara Liling Zhang Irmgard Schuiki Rui Curi Allen Volchuk Tatiana Carolina Alba-Loureiro NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells. PLoS ONE |
| title | NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells. |
| title_full | NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells. |
| title_fullStr | NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells. |
| title_full_unstemmed | NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells. |
| title_short | NADPH oxidase-dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil-like HL60 cells. |
| title_sort | nadph oxidase dependent production of reactive oxygen species induces endoplasmatic reticulum stress in neutrophil like hl60 cells |
| url | http://europepmc.org/articles/PMC4323339?pdf=render |
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