Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
Abstract Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Me...
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Format: | Article |
Language: | English |
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BMC
2019-11-01
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Series: | Microbial Cell Factories |
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Online Access: | https://doi.org/10.1186/s12934-019-1259-x |
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author | Vijaydev Ganesan Michael Spagnuolo Ayushi Agrawal Spencer Smith Difeng Gao Mark Blenner |
author_facet | Vijaydev Ganesan Michael Spagnuolo Ayushi Agrawal Spencer Smith Difeng Gao Mark Blenner |
author_sort | Vijaydev Ganesan |
collection | DOAJ |
description | Abstract Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR–Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica. In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica, discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica. |
first_indexed | 2024-12-14T23:33:40Z |
format | Article |
id | doaj.art-36997f5a9bed4f669be3158ccd081b60 |
institution | Directory Open Access Journal |
issn | 1475-2859 |
language | English |
last_indexed | 2024-12-14T23:33:40Z |
publishDate | 2019-11-01 |
publisher | BMC |
record_format | Article |
series | Microbial Cell Factories |
spelling | doaj.art-36997f5a9bed4f669be3158ccd081b602022-12-21T22:43:39ZengBMCMicrobial Cell Factories1475-28592019-11-011811910.1186/s12934-019-1259-xAdvances and opportunities in gene editing and gene regulation technology for Yarrowia lipolyticaVijaydev Ganesan0Michael Spagnuolo1Ayushi Agrawal2Spencer Smith3Difeng Gao4Mark Blenner5Department of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityAbstract Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR–Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica. In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica, discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica.https://doi.org/10.1186/s12934-019-1259-xYarrowia lipolyticaMetabolic engineeringSynthetic biologyGenetic toolsGenome editingCRISPR–Cas9 |
spellingShingle | Vijaydev Ganesan Michael Spagnuolo Ayushi Agrawal Spencer Smith Difeng Gao Mark Blenner Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica Microbial Cell Factories Yarrowia lipolytica Metabolic engineering Synthetic biology Genetic tools Genome editing CRISPR–Cas9 |
title | Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica |
title_full | Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica |
title_fullStr | Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica |
title_full_unstemmed | Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica |
title_short | Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica |
title_sort | advances and opportunities in gene editing and gene regulation technology for yarrowia lipolytica |
topic | Yarrowia lipolytica Metabolic engineering Synthetic biology Genetic tools Genome editing CRISPR–Cas9 |
url | https://doi.org/10.1186/s12934-019-1259-x |
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