Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica

Abstract Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Me...

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Main Authors: Vijaydev Ganesan, Michael Spagnuolo, Ayushi Agrawal, Spencer Smith, Difeng Gao, Mark Blenner
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Microbial Cell Factories
Subjects:
Online Access:https://doi.org/10.1186/s12934-019-1259-x
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author Vijaydev Ganesan
Michael Spagnuolo
Ayushi Agrawal
Spencer Smith
Difeng Gao
Mark Blenner
author_facet Vijaydev Ganesan
Michael Spagnuolo
Ayushi Agrawal
Spencer Smith
Difeng Gao
Mark Blenner
author_sort Vijaydev Ganesan
collection DOAJ
description Abstract Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR–Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica. In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica, discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica.
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spelling doaj.art-36997f5a9bed4f669be3158ccd081b602022-12-21T22:43:39ZengBMCMicrobial Cell Factories1475-28592019-11-011811910.1186/s12934-019-1259-xAdvances and opportunities in gene editing and gene regulation technology for Yarrowia lipolyticaVijaydev Ganesan0Michael Spagnuolo1Ayushi Agrawal2Spencer Smith3Difeng Gao4Mark Blenner5Department of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityDepartment of Chemical and Biomolecular Engineering, Clemson UniversityAbstract Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR–Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y. lipolytica. In this review we summarize the work to develop these tools and their demonstrated uses in engineering Y. lipolytica, discuss important subtleties and challenges to using these tools, and give our perspective on important gaps in gene/genome editing tools in Y. lipolytica.https://doi.org/10.1186/s12934-019-1259-xYarrowia lipolyticaMetabolic engineeringSynthetic biologyGenetic toolsGenome editingCRISPR–Cas9
spellingShingle Vijaydev Ganesan
Michael Spagnuolo
Ayushi Agrawal
Spencer Smith
Difeng Gao
Mark Blenner
Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
Microbial Cell Factories
Yarrowia lipolytica
Metabolic engineering
Synthetic biology
Genetic tools
Genome editing
CRISPR–Cas9
title Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
title_full Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
title_fullStr Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
title_full_unstemmed Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
title_short Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
title_sort advances and opportunities in gene editing and gene regulation technology for yarrowia lipolytica
topic Yarrowia lipolytica
Metabolic engineering
Synthetic biology
Genetic tools
Genome editing
CRISPR–Cas9
url https://doi.org/10.1186/s12934-019-1259-x
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