Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea)
Microplastic pollution appears to be one of the major environmental problems in the world today, and researchers have been paying special attention to the study of the impact of microplastics on biota. In this article, we studied the short-term effects of polystyrene micro-spheres on genome integrit...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/9/10/1088 |
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| author | Andrey Alexandrovich Mazur Viktor Pavlovich Chelomin Elena Vladimirovna Zhuravel Sergey Petrovich Kukla Valentina Vladimirovna Slobodskova Nadezda Vladimirovna Dovzhenko |
| author_facet | Andrey Alexandrovich Mazur Viktor Pavlovich Chelomin Elena Vladimirovna Zhuravel Sergey Petrovich Kukla Valentina Vladimirovna Slobodskova Nadezda Vladimirovna Dovzhenko |
| author_sort | Andrey Alexandrovich Mazur |
| collection | DOAJ |
| description | Microplastic pollution appears to be one of the major environmental problems in the world today, and researchers have been paying special attention to the study of the impact of microplastics on biota. In this article, we studied the short-term effects of polystyrene micro-spheres on genome integrity using the gametes of the <i>Scaphechinus mirabilis</i> sand dollar with the comet assay method. This highly sensitive method allowed us to identify the level of genome damage in both gametes before and after short-term exposure to PS microparticles. It was shown that primary polystyrene microspheres at concentrations of 10<sup>4</sup>, 10<sup>5</sup>, and 10<sup>6</sup> particles/L had a genotoxic effect during short-term exposure to the sperm of the sand dollar <i>S. mirabilis</i>, which was expressed as a significant increase in sperm DNA damage. The highest percentage of DNA damage (more than 20%) was detected in spermatozoa exposed for 1 h in water containing 105 microspheres of plastic per 1 L. Additionally, at all concentrations of microplastic studied in the experiment, the genetic damage index (GDI) values in spermatozoa exceeded the control level. However, regardless of the level of DNA damage, spermatozoa retained the ability to fertilise eggs with up to 97% efficiency. We must acknowledge that the genotoxic property of microplastic against sperm to some extent predicts the development of long-term adverse effects of environmental significance. |
| first_indexed | 2024-03-10T06:27:42Z |
| format | Article |
| id | doaj.art-369ed04126c64a79b4fcfb9c0c570cb6 |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-10T06:27:42Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-369ed04126c64a79b4fcfb9c0c570cb62023-11-22T18:45:20ZengMDPI AGJournal of Marine Science and Engineering2077-13122021-10-01910108810.3390/jmse9101088Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea)Andrey Alexandrovich Mazur0Viktor Pavlovich Chelomin1Elena Vladimirovna Zhuravel2Sergey Petrovich Kukla3Valentina Vladimirovna Slobodskova4Nadezda Vladimirovna Dovzhenko5Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaDepartment of Ecology, Far Eastern Federal University, 690922 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaIl’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, RussiaMicroplastic pollution appears to be one of the major environmental problems in the world today, and researchers have been paying special attention to the study of the impact of microplastics on biota. In this article, we studied the short-term effects of polystyrene micro-spheres on genome integrity using the gametes of the <i>Scaphechinus mirabilis</i> sand dollar with the comet assay method. This highly sensitive method allowed us to identify the level of genome damage in both gametes before and after short-term exposure to PS microparticles. It was shown that primary polystyrene microspheres at concentrations of 10<sup>4</sup>, 10<sup>5</sup>, and 10<sup>6</sup> particles/L had a genotoxic effect during short-term exposure to the sperm of the sand dollar <i>S. mirabilis</i>, which was expressed as a significant increase in sperm DNA damage. The highest percentage of DNA damage (more than 20%) was detected in spermatozoa exposed for 1 h in water containing 105 microspheres of plastic per 1 L. Additionally, at all concentrations of microplastic studied in the experiment, the genetic damage index (GDI) values in spermatozoa exceeded the control level. However, regardless of the level of DNA damage, spermatozoa retained the ability to fertilise eggs with up to 97% efficiency. We must acknowledge that the genotoxic property of microplastic against sperm to some extent predicts the development of long-term adverse effects of environmental significance.https://www.mdpi.com/2077-1312/9/10/1088microplasticPSsand dollar<i>Scaphechinus mirabilis</i>genotoxicitygametes |
| spellingShingle | Andrey Alexandrovich Mazur Viktor Pavlovich Chelomin Elena Vladimirovna Zhuravel Sergey Petrovich Kukla Valentina Vladimirovna Slobodskova Nadezda Vladimirovna Dovzhenko Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea) Journal of Marine Science and Engineering microplastic PS sand dollar <i>Scaphechinus mirabilis</i> genotoxicity gametes |
| title | Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea) |
| title_full | Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea) |
| title_fullStr | Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea) |
| title_full_unstemmed | Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea) |
| title_short | Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar <i>Scaphechinus mirabilis</i> (Agassiz, 1864) (Echinodermata, Echinoidea) |
| title_sort | genotoxicity of polystyrene ps microspheres in short term exposure to gametes of the sand dollar i scaphechinus mirabilis i agassiz 1864 echinodermata echinoidea |
| topic | microplastic PS sand dollar <i>Scaphechinus mirabilis</i> genotoxicity gametes |
| url | https://www.mdpi.com/2077-1312/9/10/1088 |
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