Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for Beetles
Ladybird beetles (Coccinellidae) are one of the largest groups of beetles. Among them, some species are of economic interest since they can act as a biological control for some agricultural pests whereas other species are phytophagous and can damage crops. <i>Chnootriba argus</i> (Coccin...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/10/9/306 |
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| author | Pablo Mora Jesús Vela Areli Ruiz-Mena Teresa Palomeque Pedro Lorite |
| author_facet | Pablo Mora Jesús Vela Areli Ruiz-Mena Teresa Palomeque Pedro Lorite |
| author_sort | Pablo Mora |
| collection | DOAJ |
| description | Ladybird beetles (Coccinellidae) are one of the largest groups of beetles. Among them, some species are of economic interest since they can act as a biological control for some agricultural pests whereas other species are phytophagous and can damage crops. <i>Chnootriba argus</i> (Coccinellidae, Epilachnini) has large heterochromatic pericentromeric blocks on all chromosomes, including both sexual chromosomes. Classical digestion of total genomic DNA using restriction endonucleases failed to find the satellite DNA located on these heterochromatic regions. Cloning of C0t-1 DNA resulted in the isolation of a repetitive DNA with a repeat unit of six base pairs, TTAAAA. The amount of TTAAAA repeat in the <i>C. argus</i> genome was about 20%. Fluorescence in situ hybridization (FISH) analysis and digestion of chromosomes with the endonuclease <i>Tru</i>9I revealed that this repetitive DNA could be considered as the putative pericentromeric satellite DNA (satDNA) in this species. The presence of this satellite DNA was tested in other species of the tribe Epilachnini and it is also present in <i>Epilachna paenulata.</i> In both species, the TTAAAA repeat seems to be the main satellite DNA and it is located on the pericentromeric region on all chromosomes. The size of this satDNA, which has only six base pairs is unusual in Coleoptera satellite DNAs, where satDNAs usually have repeat units of a much larger size. Southern hybridization and FISH proved that this satDNA is conserved in some Epilachnini species but not in others. This result is in concordance with the controversial phylogenetic relationships among the genera of the tribe Epilachnini, where the limits between genera are unclear. |
| first_indexed | 2024-12-10T05:57:37Z |
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| language | English |
| last_indexed | 2024-12-10T05:57:37Z |
| publishDate | 2019-09-01 |
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| series | Insects |
| spelling | doaj.art-369ef19266d04944bb3a0db043f67f9a2022-12-22T01:59:52ZengMDPI AGInsects2075-44502019-09-0110930610.3390/insects10090306insects10090306Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for BeetlesPablo Mora0Jesús Vela1Areli Ruiz-Mena2Teresa Palomeque3Pedro Lorite4Department of Experimental Biology, Genetic Area, University of Jaén, 23071 Jaén, SpainDepartment of Experimental Biology, Genetic Area, University of Jaén, 23071 Jaén, SpainDepartment of Experimental Biology, Genetic Area, University of Jaén, 23071 Jaén, SpainDepartment of Experimental Biology, Genetic Area, University of Jaén, 23071 Jaén, SpainDepartment of Experimental Biology, Genetic Area, University of Jaén, 23071 Jaén, SpainLadybird beetles (Coccinellidae) are one of the largest groups of beetles. Among them, some species are of economic interest since they can act as a biological control for some agricultural pests whereas other species are phytophagous and can damage crops. <i>Chnootriba argus</i> (Coccinellidae, Epilachnini) has large heterochromatic pericentromeric blocks on all chromosomes, including both sexual chromosomes. Classical digestion of total genomic DNA using restriction endonucleases failed to find the satellite DNA located on these heterochromatic regions. Cloning of C0t-1 DNA resulted in the isolation of a repetitive DNA with a repeat unit of six base pairs, TTAAAA. The amount of TTAAAA repeat in the <i>C. argus</i> genome was about 20%. Fluorescence in situ hybridization (FISH) analysis and digestion of chromosomes with the endonuclease <i>Tru</i>9I revealed that this repetitive DNA could be considered as the putative pericentromeric satellite DNA (satDNA) in this species. The presence of this satellite DNA was tested in other species of the tribe Epilachnini and it is also present in <i>Epilachna paenulata.</i> In both species, the TTAAAA repeat seems to be the main satellite DNA and it is located on the pericentromeric region on all chromosomes. The size of this satDNA, which has only six base pairs is unusual in Coleoptera satellite DNAs, where satDNAs usually have repeat units of a much larger size. Southern hybridization and FISH proved that this satDNA is conserved in some Epilachnini species but not in others. This result is in concordance with the controversial phylogenetic relationships among the genera of the tribe Epilachnini, where the limits between genera are unclear.https://www.mdpi.com/2075-4450/10/9/306ColeopteraCoccinellidaebryony ladybirdsatellite DNAC0t-1 DNAheterochromatinin situ hybridization |
| spellingShingle | Pablo Mora Jesús Vela Areli Ruiz-Mena Teresa Palomeque Pedro Lorite Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for Beetles Insects Coleoptera Coccinellidae bryony ladybird satellite DNA C0t-1 DNA heterochromatin in situ hybridization |
| title | Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for Beetles |
| title_full | Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for Beetles |
| title_fullStr | Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for Beetles |
| title_full_unstemmed | Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for Beetles |
| title_short | Isolation of a Pericentromeric Satellite DNA Family in <i>Chnootriba argus</i> (<i>Henosepilachna argus</i>) with an Unusual Short Repeat Unit (TTAAAA) for Beetles |
| title_sort | isolation of a pericentromeric satellite dna family in i chnootriba argus i i henosepilachna argus i with an unusual short repeat unit ttaaaa for beetles |
| topic | Coleoptera Coccinellidae bryony ladybird satellite DNA C0t-1 DNA heterochromatin in situ hybridization |
| url | https://www.mdpi.com/2075-4450/10/9/306 |
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