Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
Abstract The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycl...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-02668-2 |
| _version_ | 1818421950521802752 |
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| author | Ryo Onuma Neha Mishra Shin-ya Miyagishima |
| author_facet | Ryo Onuma Neha Mishra Shin-ya Miyagishima |
| author_sort | Ryo Onuma |
| collection | DOAJ |
| description | Abstract The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycle along with the chloroplast. To understand how the chloroplast, nucleomorph and host cell divide in a coordinated manner, we examined the expression of genes/proteins that are related to nucleomorph replication and chloroplast division as well as the timing of nuclear and nucleomorph DNA synthesis in the cryptophyte Guillardia theta. Nucleus-encoded nucleomorph HISTONE H2A mRNA specifically accumulated during the nuclear S phase. In contrast, nucleomorph-encoded genes/proteins that are related to nucleomorph replication and chloroplast division (FtsZ) are constantly expressed throughout the cell cycle. The results of this study and previous studies on chlorarachniophytes suggest that there was a common evolutionary pattern in which an endosymbiont lost its replication cycle-dependent transcription while cell-cycle-dependent transcriptional regulation of host nuclear genes came to restrict the timing of nucleomorph replication and chloroplast division. |
| first_indexed | 2024-12-14T13:18:30Z |
| format | Article |
| id | doaj.art-369f2048556143d6bc0b6325f794114b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T13:18:30Z |
| publishDate | 2017-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-369f2048556143d6bc0b6325f794114b2022-12-21T22:59:59ZengNature PortfolioScientific Reports2045-23222017-05-017111210.1038/s41598-017-02668-2Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia thetaRyo Onuma0Neha Mishra1Shin-ya Miyagishima2Department of Cell Genetics, National Institute of GeneticsDepartment of Cell Genetics, National Institute of GeneticsDepartment of Cell Genetics, National Institute of GeneticsAbstract The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant of the endosymbiont nucleus (nucleomorph) that is replicated once in the cell cycle along with the chloroplast. To understand how the chloroplast, nucleomorph and host cell divide in a coordinated manner, we examined the expression of genes/proteins that are related to nucleomorph replication and chloroplast division as well as the timing of nuclear and nucleomorph DNA synthesis in the cryptophyte Guillardia theta. Nucleus-encoded nucleomorph HISTONE H2A mRNA specifically accumulated during the nuclear S phase. In contrast, nucleomorph-encoded genes/proteins that are related to nucleomorph replication and chloroplast division (FtsZ) are constantly expressed throughout the cell cycle. The results of this study and previous studies on chlorarachniophytes suggest that there was a common evolutionary pattern in which an endosymbiont lost its replication cycle-dependent transcription while cell-cycle-dependent transcriptional regulation of host nuclear genes came to restrict the timing of nucleomorph replication and chloroplast division.https://doi.org/10.1038/s41598-017-02668-2 |
| spellingShingle | Ryo Onuma Neha Mishra Shin-ya Miyagishima Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta Scientific Reports |
| title | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
| title_full | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
| title_fullStr | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
| title_full_unstemmed | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
| title_short | Regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta |
| title_sort | regulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte guillardia theta |
| url | https://doi.org/10.1038/s41598-017-02668-2 |
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