Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra)
<i>Calloselasma rhodostoma</i> (CR) and <i>Ophiophagus hannah</i> (OH) are two medically important snakes found in Malaysia. While some studies have described the biological properties of these venoms, feeding and environmental conditions also influence the concentration and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/10/11/434 |
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| author | Sugita Kunalan Iekhsan Othman Sharifah Syed Hassan Wayne C. Hodgson |
| author_facet | Sugita Kunalan Iekhsan Othman Sharifah Syed Hassan Wayne C. Hodgson |
| author_sort | Sugita Kunalan |
| collection | DOAJ |
| description | <i>Calloselasma rhodostoma</i> (CR) and <i>Ophiophagus hannah</i> (OH) are two medically important snakes found in Malaysia. While some studies have described the biological properties of these venoms, feeding and environmental conditions also influence the concentration and distribution of snake venom toxins, resulting in variations in venom composition. Therefore, a combined proteomic approach using shotgun and gel filtration chromatography, analyzed by tandem mass spectrometry, was used to examine the composition of venoms from these Malaysian snakes. The analysis revealed 114 proteins (15 toxin families) and 176 proteins (20 toxin families) in Malaysian <i>Calloselasma rhodostoma</i> and <i>Ophiophagus hannah</i> species, respectively. Flavin monoamine oxidase, phospholipase A<sub>2</sub>, phosphodiesterase, snake venom metalloproteinase, and serine protease toxin families were identified in both venoms. Aminopeptidase, glutaminyl-peptide cyclotransferase along with ankyrin repeats were identified for the first time in CR venom, and insulin, c-type lectins/snaclecs, hepatocyte growth factor, and macrophage colony-stimulating factor together with tumor necrosis factor were identified in OH venom for the first time. Our combined proteomic approach has identified a comprehensive arsenal of toxins in CR and OH venoms. These data may be utilized for improved antivenom production, understanding pathological effects of envenoming, and the discovery of biologically active peptides with medical and/or biotechnological value. |
| first_indexed | 2024-04-11T13:41:47Z |
| format | Article |
| id | doaj.art-36a068becb5745e48c21a81e78db891d |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:41:47Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-36a068becb5745e48c21a81e78db891d2022-12-22T04:21:14ZengMDPI AGToxins2072-66512018-10-01101143410.3390/toxins10110434toxins10110434Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra)Sugita Kunalan0Iekhsan Othman1Sharifah Syed Hassan2Wayne C. Hodgson3Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, MalaysiaJeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, MalaysiaJeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan 47500, MalaysiaMonash Venom Group, Department of Pharmacology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Victoria 3800, Australia<i>Calloselasma rhodostoma</i> (CR) and <i>Ophiophagus hannah</i> (OH) are two medically important snakes found in Malaysia. While some studies have described the biological properties of these venoms, feeding and environmental conditions also influence the concentration and distribution of snake venom toxins, resulting in variations in venom composition. Therefore, a combined proteomic approach using shotgun and gel filtration chromatography, analyzed by tandem mass spectrometry, was used to examine the composition of venoms from these Malaysian snakes. The analysis revealed 114 proteins (15 toxin families) and 176 proteins (20 toxin families) in Malaysian <i>Calloselasma rhodostoma</i> and <i>Ophiophagus hannah</i> species, respectively. Flavin monoamine oxidase, phospholipase A<sub>2</sub>, phosphodiesterase, snake venom metalloproteinase, and serine protease toxin families were identified in both venoms. Aminopeptidase, glutaminyl-peptide cyclotransferase along with ankyrin repeats were identified for the first time in CR venom, and insulin, c-type lectins/snaclecs, hepatocyte growth factor, and macrophage colony-stimulating factor together with tumor necrosis factor were identified in OH venom for the first time. Our combined proteomic approach has identified a comprehensive arsenal of toxins in CR and OH venoms. These data may be utilized for improved antivenom production, understanding pathological effects of envenoming, and the discovery of biologically active peptides with medical and/or biotechnological value.https://www.mdpi.com/2072-6651/10/11/434<i>Calloselasma rhodostoma</i><i>Ophiophagus hannah</i>toxinproteomicsvenomicsvenom proteome |
| spellingShingle | Sugita Kunalan Iekhsan Othman Sharifah Syed Hassan Wayne C. Hodgson Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra) Toxins <i>Calloselasma rhodostoma</i> <i>Ophiophagus hannah</i> toxin proteomics venomics venom proteome |
| title | Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra) |
| title_full | Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra) |
| title_fullStr | Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra) |
| title_full_unstemmed | Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra) |
| title_short | Proteomic Characterization of Two Medically Important Malaysian Snake Venoms, <i>Calloselasma rhodostoma</i> (Malayan Pit Viper) and <i>Ophiophagus hannah</i> (King Cobra) |
| title_sort | proteomic characterization of two medically important malaysian snake venoms i calloselasma rhodostoma i malayan pit viper and i ophiophagus hannah i king cobra |
| topic | <i>Calloselasma rhodostoma</i> <i>Ophiophagus hannah</i> toxin proteomics venomics venom proteome |
| url | https://www.mdpi.com/2072-6651/10/11/434 |
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