A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid Chromatography
Pyrethroid residues in traditional Chinese medicines have been a serious threat to the health and treatment of patients. However, because of the matrix complexity of traditional Chinese medicine, the detection of pyrethroid residues remains a challenge. Therefore, we developed a QuEChERS method coup...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/8/1470 |
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| author | Yuge Hou Hong Chen Xin Li Yiyi Liao Makoto Tsunoda Yingxia Zhang Shiming Deng Yanting Song |
| author_facet | Yuge Hou Hong Chen Xin Li Yiyi Liao Makoto Tsunoda Yingxia Zhang Shiming Deng Yanting Song |
| author_sort | Yuge Hou |
| collection | DOAJ |
| description | Pyrethroid residues in traditional Chinese medicines have been a serious threat to the health and treatment of patients. However, because of the matrix complexity of traditional Chinese medicine, the detection of pyrethroid residues remains a challenge. Therefore, we developed a QuEChERS method coupled with high-performance liquid chromatography and ultraviolet detection (HPLC-UV) for the determination of pyrethroid pesticides in three kinds of traditional Chinese medicine oral liquid preparations, and we investigated and optimized the extraction conditions. The matrix effect was estimated in the organic solvent and the actual samples by comparing the slopes of calibration curves, and the results showed that the matrix effect is not significant when using the modified QuEChERS method. The pyrethroid pesticides could be completely separated in 30 min. The linear correlation coefficients were more than 0.999, and the recoveries of all the pyrethroid pesticides ranged from 87.2% to 104.8%. The intra-day precisions (n = 5) were 2.44–4.62%, and the inter-day precisions (n = 5) were 1.06–3.02%. Moreover, the limits of detection were in the range of 0.007–0.018 ng mL<sup>−1</sup>, while the limits of quantitation were in the range of 0.022–0.057 ng mL<sup>−1</sup>. This simple, low-cost, and highly sensitive analytical method can be a potential tool for the analysis of pyrethroid residues in traditional Chinese medicine oral liquid preparations. |
| first_indexed | 2024-12-13T01:29:13Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T01:29:13Z |
| publishDate | 2019-04-01 |
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| series | Molecules |
| spelling | doaj.art-36a14fd3a6b4406ba0a966f8508e63f82022-12-22T00:04:03ZengMDPI AGMolecules1420-30492019-04-01248147010.3390/molecules24081470molecules24081470A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid ChromatographyYuge Hou0Hong Chen1Xin Li2Yiyi Liao3Makoto Tsunoda4Yingxia Zhang5Shiming Deng6Yanting Song7Key Laboratory of Tropical Biological Resources of Ministry of Education, Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education, Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education, Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education, Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaGraduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, JapanKey Laboratory of Tropical Biological Resources of Ministry of Education, Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education, Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaKey Laboratory of Tropical Biological Resources of Ministry of Education, Department of Pharmaceutical Sciences, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, ChinaPyrethroid residues in traditional Chinese medicines have been a serious threat to the health and treatment of patients. However, because of the matrix complexity of traditional Chinese medicine, the detection of pyrethroid residues remains a challenge. Therefore, we developed a QuEChERS method coupled with high-performance liquid chromatography and ultraviolet detection (HPLC-UV) for the determination of pyrethroid pesticides in three kinds of traditional Chinese medicine oral liquid preparations, and we investigated and optimized the extraction conditions. The matrix effect was estimated in the organic solvent and the actual samples by comparing the slopes of calibration curves, and the results showed that the matrix effect is not significant when using the modified QuEChERS method. The pyrethroid pesticides could be completely separated in 30 min. The linear correlation coefficients were more than 0.999, and the recoveries of all the pyrethroid pesticides ranged from 87.2% to 104.8%. The intra-day precisions (n = 5) were 2.44–4.62%, and the inter-day precisions (n = 5) were 1.06–3.02%. Moreover, the limits of detection were in the range of 0.007–0.018 ng mL<sup>−1</sup>, while the limits of quantitation were in the range of 0.022–0.057 ng mL<sup>−1</sup>. This simple, low-cost, and highly sensitive analytical method can be a potential tool for the analysis of pyrethroid residues in traditional Chinese medicine oral liquid preparations.https://www.mdpi.com/1420-3049/24/8/1470traditional Chinese medicine oral liquidpyrethroid pesticideQuEChERShigh-performance liquid chromatography |
| spellingShingle | Yuge Hou Hong Chen Xin Li Yiyi Liao Makoto Tsunoda Yingxia Zhang Shiming Deng Yanting Song A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid Chromatography Molecules traditional Chinese medicine oral liquid pyrethroid pesticide QuEChERS high-performance liquid chromatography |
| title | A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid Chromatography |
| title_full | A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid Chromatography |
| title_fullStr | A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid Chromatography |
| title_full_unstemmed | A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid Chromatography |
| title_short | A Modified QuEChERS Method for Determination of Pyrethroid Residues in Traditional Chinese Medicine Oral Liquids by High-Performance Liquid Chromatography |
| title_sort | modified quechers method for determination of pyrethroid residues in traditional chinese medicine oral liquids by high performance liquid chromatography |
| topic | traditional Chinese medicine oral liquid pyrethroid pesticide QuEChERS high-performance liquid chromatography |
| url | https://www.mdpi.com/1420-3049/24/8/1470 |
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