Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients
Objectives: The present study was aimed to investigate the antibiotic resistance, virulence potential and phylogenetic grouping of ESBL-producing uropathogenic Escherichia coli (EP-UPEC) isolated from long-term hospitalized patients. Materials and Methods: EP-UPEC isolates from September 2013 t...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/6157/13234_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1818962553899843584 |
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| author | Ruike Zhao Jinfang Shi Yimin Shen Yanmeng Li Qingzhen Han Xianfeng Zhang Guohao Gu Jie Xu |
| author_facet | Ruike Zhao Jinfang Shi Yimin Shen Yanmeng Li Qingzhen Han Xianfeng Zhang Guohao Gu Jie Xu |
| author_sort | Ruike Zhao |
| collection | DOAJ |
| description | Objectives: The present study was aimed to investigate the
antibiotic resistance, virulence potential and phylogenetic grouping
of ESBL-producing uropathogenic Escherichia coli (EP-UPEC)
isolated from long-term hospitalized patients.
Materials and Methods: EP-UPEC isolates from September
2013 to June 2014 at a tertiary care hospital of China were
screened for ESBL-production by the double disk diffusion test.
Isolates with ESBL-phenotype were further characterized by
antibiotic resistance testing, PCR of different ESBL and virulence
genes, and phylogenetic grouping.
Results: One hundred and twenty EP-UPEC were isolated from
long-term hospitalized patients. All EP-UPEC isolates were resistant
to Ampicillin, Cefazolin, Cefuroxime, Cefotaxime, Cefoperazone and
Ceftriaxone, and the majority of EP-UPEC isolates were resistant
to Piperacillin (82.5%), Ciprofloxacin (81.2%), TrimethoprimSulfamethoxazole (72.5%). The isolates showed the highest
sensitivity against Imipenem (98.4%), Piperacillin/tazobactam
(96.7%), Cefoperazone/sulbactam (91.7%), Amikacin (90.8%)
and Cefepime (75.8%). Nine different ESBL genotype patterns
were observed and CTX-M type was the most prevalent ESBL
genotype (42.5%, 51/120). Majority of EP-UPEC isolates possess
more than one ESBL genes. EP-UPEC isolates belonged mainly
to phylogenetic group B2(36.7%) and D(35.0%). The prevalence
of traT, ompT, iss, PAI, afa, fimH and papC were 75.8%, 63.3%,
63.3%, 60.8%, 40.8%, 19.2% and 6.7%, respectively. The number
of virulence genes (VGs) detected was significantly higher in group
B2 than in group A (ANOVA, p<0.001), group B1(ANOVA, p= 0.012)
and D (ANOVA, p<0.001).
Conclusions: EP-UPEC strains showed multidrug resistance and
co-resistance to other non β-lactam antibiotics. CTX-M was the
most prevalent ESBL genotype and majority of EP-UPEC strains
more than one ESBL genes. EP-UPEC strains belonged mainly to
phylogenetic group B2 and D, and most of the virulence genes
were more prevalent in group B2. |
| first_indexed | 2024-12-20T12:31:10Z |
| format | Article |
| id | doaj.art-36a30dcf12a448bc97fd0614cf929679 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-20T12:31:10Z |
| publishDate | 2015-07-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-36a30dcf12a448bc97fd0614cf9296792022-12-21T19:40:43ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-07-0197DC01DC0410.7860/JCDR/2015/13234.6157Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized PatientsRuike Zhao0Jinfang Shi1Yimin Shen2Yanmeng Li3Qingzhen Han4Xianfeng Zhang5Guohao Gu6Jie Xu7Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University, Suzhou, P.R. of China.Objectives: The present study was aimed to investigate the antibiotic resistance, virulence potential and phylogenetic grouping of ESBL-producing uropathogenic Escherichia coli (EP-UPEC) isolated from long-term hospitalized patients. Materials and Methods: EP-UPEC isolates from September 2013 to June 2014 at a tertiary care hospital of China were screened for ESBL-production by the double disk diffusion test. Isolates with ESBL-phenotype were further characterized by antibiotic resistance testing, PCR of different ESBL and virulence genes, and phylogenetic grouping. Results: One hundred and twenty EP-UPEC were isolated from long-term hospitalized patients. All EP-UPEC isolates were resistant to Ampicillin, Cefazolin, Cefuroxime, Cefotaxime, Cefoperazone and Ceftriaxone, and the majority of EP-UPEC isolates were resistant to Piperacillin (82.5%), Ciprofloxacin (81.2%), TrimethoprimSulfamethoxazole (72.5%). The isolates showed the highest sensitivity against Imipenem (98.4%), Piperacillin/tazobactam (96.7%), Cefoperazone/sulbactam (91.7%), Amikacin (90.8%) and Cefepime (75.8%). Nine different ESBL genotype patterns were observed and CTX-M type was the most prevalent ESBL genotype (42.5%, 51/120). Majority of EP-UPEC isolates possess more than one ESBL genes. EP-UPEC isolates belonged mainly to phylogenetic group B2(36.7%) and D(35.0%). The prevalence of traT, ompT, iss, PAI, afa, fimH and papC were 75.8%, 63.3%, 63.3%, 60.8%, 40.8%, 19.2% and 6.7%, respectively. The number of virulence genes (VGs) detected was significantly higher in group B2 than in group A (ANOVA, p<0.001), group B1(ANOVA, p= 0.012) and D (ANOVA, p<0.001). Conclusions: EP-UPEC strains showed multidrug resistance and co-resistance to other non β-lactam antibiotics. CTX-M was the most prevalent ESBL genotype and majority of EP-UPEC strains more than one ESBL genes. EP-UPEC strains belonged mainly to phylogenetic group B2 and D, and most of the virulence genes were more prevalent in group B2.https://jcdr.net/articles/PDF/6157/13234_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdfesblphylogenetic groupsresistanceupecvirulence |
| spellingShingle | Ruike Zhao Jinfang Shi Yimin Shen Yanmeng Li Qingzhen Han Xianfeng Zhang Guohao Gu Jie Xu Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients Journal of Clinical and Diagnostic Research esbl phylogenetic groups resistance upec virulence |
| title | Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients |
| title_full | Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients |
| title_fullStr | Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients |
| title_full_unstemmed | Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients |
| title_short | Phylogenetic Distribution of Virulence Genes Among ESBL-producing Uropathogenic Escherichia coli Isolated from Long-term Hospitalized Patients |
| title_sort | phylogenetic distribution of virulence genes among esbl producing uropathogenic escherichia coli isolated from long term hospitalized patients |
| topic | esbl phylogenetic groups resistance upec virulence |
| url | https://jcdr.net/articles/PDF/6157/13234_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
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