The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudoviruses
Although most Coronavirus disease (COVID-19) patients can recover fully, the disease remains a significant cause of morbidity and mortality. In addition to the consequences of acute infection, a proportion of the population experiences long-term adverse effects associated with SARS-CoV-2. Therefore,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2023-12-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/16234.pdf |
| _version_ | 1797400509159571456 |
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| author | Conglian Tan Nian Wang Shanshan Deng Xiaoheng Wu Changwu Yue Xu Jia Yuhong Lyu |
| author_facet | Conglian Tan Nian Wang Shanshan Deng Xiaoheng Wu Changwu Yue Xu Jia Yuhong Lyu |
| author_sort | Conglian Tan |
| collection | DOAJ |
| description | Although most Coronavirus disease (COVID-19) patients can recover fully, the disease remains a significant cause of morbidity and mortality. In addition to the consequences of acute infection, a proportion of the population experiences long-term adverse effects associated with SARS-CoV-2. Therefore, it is still critical to comprehend the virus’s characteristics and how it interacts with its host to develop effective drugs and vaccines against COVID-19. SARS-CoV-2 pseudovirus, a replication-deficient recombinant glycoprotein chimeric viral particle, enables investigations of highly pathogenic viruses to be conducted without the constraint of high-level biosafety facilities, considerably advancing virology and being extensively employed in the study of SARS-CoV-2. This review summarizes three methods of establishing SARS-CoV-2 pseudovirus and current knowledge in vaccine development, neutralizing antibody research, and antiviral drug screening, as well as recent progress in virus entry mechanism and susceptible cell screening. We also discuss the potential advantages and disadvantages. |
| first_indexed | 2024-03-09T01:56:25Z |
| format | Article |
| id | doaj.art-36a4e304e5ed40ea8ba911c93c4167c2 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T01:56:25Z |
| publishDate | 2023-12-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-36a4e304e5ed40ea8ba911c93c4167c22023-12-08T15:05:06ZengPeerJ Inc.PeerJ2167-83592023-12-0111e1623410.7717/peerj.16234The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudovirusesConglian Tan0Nian Wang1Shanshan Deng2Xiaoheng Wu3Changwu Yue4Xu Jia5Yuhong Lyu6Key Laboratory of Microbial Drugs Innovation and Transformation, Medical College, Yan’an University, Yan’an, Shaanxi, ChinaChengdu Medical College, Chengdu, Sichuan, ChinaNon-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical College, Chengdu, Sichuan, ChinaKey Laboratory of Microbial Drugs Innovation and Transformation, Medical College, Yan’an University, Yan’an, Shaanxi, ChinaKey Laboratory of Microbial Drugs Innovation and Transformation, Medical College, Yan’an University, Yan’an, Shaanxi, ChinaNon-coding RNA and Drug Discovery Key Laboratory of Sichuan Province, Chengdu Medical College, Chengdu, Sichuan, ChinaKey Laboratory of Microbial Drugs Innovation and Transformation, Medical College, Yan’an University, Yan’an, Shaanxi, ChinaAlthough most Coronavirus disease (COVID-19) patients can recover fully, the disease remains a significant cause of morbidity and mortality. In addition to the consequences of acute infection, a proportion of the population experiences long-term adverse effects associated with SARS-CoV-2. Therefore, it is still critical to comprehend the virus’s characteristics and how it interacts with its host to develop effective drugs and vaccines against COVID-19. SARS-CoV-2 pseudovirus, a replication-deficient recombinant glycoprotein chimeric viral particle, enables investigations of highly pathogenic viruses to be conducted without the constraint of high-level biosafety facilities, considerably advancing virology and being extensively employed in the study of SARS-CoV-2. This review summarizes three methods of establishing SARS-CoV-2 pseudovirus and current knowledge in vaccine development, neutralizing antibody research, and antiviral drug screening, as well as recent progress in virus entry mechanism and susceptible cell screening. We also discuss the potential advantages and disadvantages.https://peerj.com/articles/16234.pdfSARS-CoV-2 pseudovirus packing systemNeutralizing antibodyVaccineSARS-CoV-2 variantDrugVirus-host interactions |
| spellingShingle | Conglian Tan Nian Wang Shanshan Deng Xiaoheng Wu Changwu Yue Xu Jia Yuhong Lyu The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudoviruses PeerJ SARS-CoV-2 pseudovirus packing system Neutralizing antibody Vaccine SARS-CoV-2 variant Drug Virus-host interactions |
| title | The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudoviruses |
| title_full | The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudoviruses |
| title_fullStr | The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudoviruses |
| title_full_unstemmed | The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudoviruses |
| title_short | The development and application of pseudoviruses: assessment of SARS-CoV-2 pseudoviruses |
| title_sort | development and application of pseudoviruses assessment of sars cov 2 pseudoviruses |
| topic | SARS-CoV-2 pseudovirus packing system Neutralizing antibody Vaccine SARS-CoV-2 variant Drug Virus-host interactions |
| url | https://peerj.com/articles/16234.pdf |
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