Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro
Objective — to find out the peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro. Material and methods. The research was conducted in the lab microclonal reproduction of the National Dendropark Sofiyivka of the NAS of Ukraine. The method of microclonal propagation o...
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| Format: | Article |
| Language: | English |
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NAS of Ukraine, M.M. Gryshko National Botanical Garden
2019-12-01
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| Series: | Інтродукція Рослин |
| Subjects: | |
| Online Access: | https://plantintroduction.org/index.php/pi/article/view/1530 |
| _version_ | 1828433098428121088 |
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| author | М.V. Nebykov L.А. Koldar N.V. Derevianko |
| author_facet | М.V. Nebykov L.А. Koldar N.V. Derevianko |
| author_sort | М.V. Nebykov |
| collection | DOAJ |
| description | Objective — to find out the peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro.
Material and methods. The research was conducted in the lab microclonal reproduction of the National Dendropark Sofiyivka of the NAS of Ukraine. The method of microclonal propagation of plants was used, it base on the induction of morphogenic processes that occurred under the influence of phytohormones. The material for research was young, not well-grown shoots taken from 3-year-old plants P. laurocerasus, P. laurocerasus ‘Serbica’, P. laurocerasus ‘Schipcaensis’, P. laurocerasus ‘03.02’ obtained from Novokakhovsky research farm of the Institute of rice of the NAAS of Ukraine.
Results. The results of three-year research on the reproduction characteristics of P. laurocerasus, P. laurocerasus ‘Serbica’, P. laurocerasus ‘Schipcaensis’, P. laurocerasus ‘03.02’ in vitro are presented on the use of modified nutrient varieties with different phytohormonal composition. The dependence of the differentiation of explants in these genotypes and processes of morphogenesis, on the exogenous content of phytohormones: 6-benzylaminopurine (6-BAP), indolyl-3-butyric acid (IBA), indolyl-3-acetic acid, 1-naphthylacetic acid (1-NAA) in nutrient media, which contributed to the accelerated appearance of adventitious kidneys. It was found that for P. laurocerasus, P. laurocerasus ‘Schipcaensis’ and P. laurocerasus ‘03.02 the most optimal was the medium Murashige–Scoog (MS-6) to which was added 1.0 mg/l 6-BAP: and the average number of formed shoots respectively was 5.63, 4.66 and 6.61, the average length of shoots was 2.3 cm, and the reproduction coefficient was respectively 6.56, 5.34 and 7.24. For P. laurocerasus ‘Serbica’ the use of the MS-7 medium with the addition of 1.0 mg/l 6-BAP and 0.1 mg/l IBA was effective, which contributed to an average of 4.77 pieces shoots in length 2.09 cm with a multiplication factor of 4.98. Well-developed shoots were transferred to nutrient media for rhizogenesis. The most effective was the nutrient medium of MS with the addition 0.5 and 1.0 mg/l 1-NAA where the rooting of explants respectively was 73.2 and 59.5 %.
Conclusions. It has been found that for P. laurocerasus, P. laurocerasus ‘Schipcaensis’ and P. laurocerasus ‘03.02 ‘ the nutrient medium, to which was added 1.0 mg/l 6-BAP, was found to be the most optimal, for P. laurocerasus ‘Serbica’ — nutrient medium with the addition of 1.0 mg/l 6-BAP and 0.1 mg/l IBA. For rhizogenesis the nutrient medium with the addition 0.5 and 1.0 mg/l of 1-NAA was found to be most effective. |
| first_indexed | 2024-12-10T18:30:24Z |
| format | Article |
| id | doaj.art-36a52cec82fe4b568a139aa555c37f7a |
| institution | Directory Open Access Journal |
| issn | 1605-6574 2663-290X |
| language | English |
| last_indexed | 2024-12-10T18:30:24Z |
| publishDate | 2019-12-01 |
| publisher | NAS of Ukraine, M.M. Gryshko National Botanical Garden |
| record_format | Article |
| series | Інтродукція Рослин |
| spelling | doaj.art-36a52cec82fe4b568a139aa555c37f7a2022-12-22T01:37:58ZengNAS of Ukraine, M.M. Gryshko National Botanical GardenІнтродукція Рослин1605-65742663-290X2019-12-018410.5281/zenodo.3566618Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitroМ.V. Nebykov0L.А. Koldar1N.V. Derevianko2National Dendrological Park Sofiyivka, National Academy Sciences of Ukraine, Ukraine, Cherkassy Region, UmanNational Dendrological Park Sofiyivka, National Academy Sciences of Ukraine, Ukraine, Cherkassy Region, UmanInstitute of Rice, National Academy of Agrarion Sciences of Ukraine, Ukraine, Kherson Region, Skadovsk District, AntonivkaObjective — to find out the peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro. Material and methods. The research was conducted in the lab microclonal reproduction of the National Dendropark Sofiyivka of the NAS of Ukraine. The method of microclonal propagation of plants was used, it base on the induction of morphogenic processes that occurred under the influence of phytohormones. The material for research was young, not well-grown shoots taken from 3-year-old plants P. laurocerasus, P. laurocerasus ‘Serbica’, P. laurocerasus ‘Schipcaensis’, P. laurocerasus ‘03.02’ obtained from Novokakhovsky research farm of the Institute of rice of the NAAS of Ukraine. Results. The results of three-year research on the reproduction characteristics of P. laurocerasus, P. laurocerasus ‘Serbica’, P. laurocerasus ‘Schipcaensis’, P. laurocerasus ‘03.02’ in vitro are presented on the use of modified nutrient varieties with different phytohormonal composition. The dependence of the differentiation of explants in these genotypes and processes of morphogenesis, on the exogenous content of phytohormones: 6-benzylaminopurine (6-BAP), indolyl-3-butyric acid (IBA), indolyl-3-acetic acid, 1-naphthylacetic acid (1-NAA) in nutrient media, which contributed to the accelerated appearance of adventitious kidneys. It was found that for P. laurocerasus, P. laurocerasus ‘Schipcaensis’ and P. laurocerasus ‘03.02 the most optimal was the medium Murashige–Scoog (MS-6) to which was added 1.0 mg/l 6-BAP: and the average number of formed shoots respectively was 5.63, 4.66 and 6.61, the average length of shoots was 2.3 cm, and the reproduction coefficient was respectively 6.56, 5.34 and 7.24. For P. laurocerasus ‘Serbica’ the use of the MS-7 medium with the addition of 1.0 mg/l 6-BAP and 0.1 mg/l IBA was effective, which contributed to an average of 4.77 pieces shoots in length 2.09 cm with a multiplication factor of 4.98. Well-developed shoots were transferred to nutrient media for rhizogenesis. The most effective was the nutrient medium of MS with the addition 0.5 and 1.0 mg/l 1-NAA where the rooting of explants respectively was 73.2 and 59.5 %. Conclusions. It has been found that for P. laurocerasus, P. laurocerasus ‘Schipcaensis’ and P. laurocerasus ‘03.02 ‘ the nutrient medium, to which was added 1.0 mg/l 6-BAP, was found to be the most optimal, for P. laurocerasus ‘Serbica’ — nutrient medium with the addition of 1.0 mg/l 6-BAP and 0.1 mg/l IBA. For rhizogenesis the nutrient medium with the addition 0.5 and 1.0 mg/l of 1-NAA was found to be most effective.https://plantintroduction.org/index.php/pi/article/view/1530in vitro propagationPrunus laurocerasusdecorative formsphytohormonesmorphogenesisregeneration plants |
| spellingShingle | М.V. Nebykov L.А. Koldar N.V. Derevianko Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro Інтродукція Рослин in vitro propagation Prunus laurocerasus decorative forms phytohormones morphogenesis regeneration plants |
| title | Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro |
| title_full | Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro |
| title_fullStr | Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro |
| title_full_unstemmed | Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro |
| title_short | Peculiarities of reproduction of representatives of Prunus laurocerasus L. in vitro |
| title_sort | peculiarities of reproduction of representatives of prunus laurocerasus l in vitro |
| topic | in vitro propagation Prunus laurocerasus decorative forms phytohormones morphogenesis regeneration plants |
| url | https://plantintroduction.org/index.php/pi/article/view/1530 |
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