Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation
Abstract Background Phosphorus (P) is an essential element of microalgae, which is either required for anabolism or for energy metabolism. When employing a nitrogen limitation strategy to trigger microalgal intracellular lipid accumulation, P supplementation was always simultaneously applied to comp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1458-z |
| _version_ | 1811233325502693376 |
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| author | Liang Fu Qingcheng Li Ge Yan Dandan Zhou John C. Crittenden |
| author_facet | Liang Fu Qingcheng Li Ge Yan Dandan Zhou John C. Crittenden |
| author_sort | Liang Fu |
| collection | DOAJ |
| description | Abstract Background Phosphorus (P) is an essential element of microalgae, which is either required for anabolism or for energy metabolism. When employing a nitrogen limitation strategy to trigger microalgal intracellular lipid accumulation, P supplementation was always simultaneously applied to compensate for the accompanied growth inhibition. Results This study identified that P exerts hormesis effects on microalgae. Slight excess of P (≤ 45 mg L−1) under nitrogen limitation condition stimulated the cell growth of Chlorella regularis and achieved a 10.2% biomass production increase. This also improved mitochondrial activity by 25.0% compared to control (P = 5.4 mg L−1). The lipid productivity reached 354.38 mg (L d)−1, which increased by 39.3% compared to control. Such an improvement was caused by the intracellularly stored polyphosphate energy pool. However, large excess of P (250 mg L−1) inhibited the cell growth by 38.8% and mitochondrial activity decreased by 71.3%. C. regularis cells showed obvious poisoning status, such as enlarged size, plasmolysis, deformation of cell walls, and disorganization of organelles. This is probably because the over-accumulated P protonated the amide-N and disrupted membrane permeability. Conclusions These results provide new insight into the roles of P in microalgae lipid production: P does not always play a positive role under nitrogen limitation conditions. |
| first_indexed | 2024-04-12T11:18:46Z |
| format | Article |
| id | doaj.art-36a53b0c075549039dbd2ec6c0083876 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-12T11:18:46Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-36a53b0c075549039dbd2ec6c00838762022-12-22T03:35:26ZengBMCBiotechnology for Biofuels1754-68342019-05-011211910.1186/s13068-019-1458-zHormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitationLiang Fu0Qingcheng Li1Ge Yan2Dandan Zhou3John C. Crittenden4Engineering Lab for Water Pollution Control and Resources Recovery, School of Environment, Northeast Normal UniversityEngineering Lab for Water Pollution Control and Resources Recovery, School of Environment, Northeast Normal UniversityEngineering Lab for Water Pollution Control and Resources Recovery, School of Environment, Northeast Normal UniversityEngineering Lab for Water Pollution Control and Resources Recovery, School of Environment, Northeast Normal UniversityEngineering Lab for Water Pollution Control and Resources Recovery, School of Environment, Northeast Normal UniversityAbstract Background Phosphorus (P) is an essential element of microalgae, which is either required for anabolism or for energy metabolism. When employing a nitrogen limitation strategy to trigger microalgal intracellular lipid accumulation, P supplementation was always simultaneously applied to compensate for the accompanied growth inhibition. Results This study identified that P exerts hormesis effects on microalgae. Slight excess of P (≤ 45 mg L−1) under nitrogen limitation condition stimulated the cell growth of Chlorella regularis and achieved a 10.2% biomass production increase. This also improved mitochondrial activity by 25.0% compared to control (P = 5.4 mg L−1). The lipid productivity reached 354.38 mg (L d)−1, which increased by 39.3% compared to control. Such an improvement was caused by the intracellularly stored polyphosphate energy pool. However, large excess of P (250 mg L−1) inhibited the cell growth by 38.8% and mitochondrial activity decreased by 71.3%. C. regularis cells showed obvious poisoning status, such as enlarged size, plasmolysis, deformation of cell walls, and disorganization of organelles. This is probably because the over-accumulated P protonated the amide-N and disrupted membrane permeability. Conclusions These results provide new insight into the roles of P in microalgae lipid production: P does not always play a positive role under nitrogen limitation conditions.http://link.springer.com/article/10.1186/s13068-019-1458-zMicroalgaePhosphorusHormesisNitrogen limitationToxic |
| spellingShingle | Liang Fu Qingcheng Li Ge Yan Dandan Zhou John C. Crittenden Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation Biotechnology for Biofuels Microalgae Phosphorus Hormesis Nitrogen limitation Toxic |
| title | Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation |
| title_full | Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation |
| title_fullStr | Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation |
| title_full_unstemmed | Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation |
| title_short | Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation |
| title_sort | hormesis effects of phosphorus on the viability of chlorella regularis cells under nitrogen limitation |
| topic | Microalgae Phosphorus Hormesis Nitrogen limitation Toxic |
| url | http://link.springer.com/article/10.1186/s13068-019-1458-z |
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