A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of Meningitis
Objective: Tuberculosis kills 3.70 lakh patients in India every year,out of which 7-12 % are meningeal involvement. Delay in its diagnosis and initiation of treatment results in poor prognosis and squeal in up to 25% of cases. The aim of the present study is to look for a simple, rapid, cost eff...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2014-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/4012/23-%207154_E(Ra)_F(P)_PF1(PAK)_PFA(AK)_PF2(PAK).pdf |
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| author | Ashok Kumar Agarwal Sonia Bansal Vidya Nand |
| author_facet | Ashok Kumar Agarwal Sonia Bansal Vidya Nand |
| author_sort | Ashok Kumar Agarwal |
| collection | DOAJ |
| description | Objective: Tuberculosis kills 3.70 lakh patients in India every
year,out of which 7-12 % are meningeal involvement. Delay in
its diagnosis and initiation of treatment results in poor prognosis
and squeal in up to 25% of cases. The aim of the present study
is to look for a simple, rapid, cost effective, and fairly specific
test in differentiating tubercular aetiology from other causes of
meningitis. In the present study we measured the adenosine
deaminase activity (ADA) in Cerebrospinal Fluid (CSF) of
Tubercular Meningitis (TBM) and non-TBM patients.
Methods: Fifty six patients attending hospital with symptoms
and signs of meningitis were selected and divided into three
groups: tubercular, pyogenic, and aseptic meningitis, depending
upon the accepted criteria. CSF was drawn and ADA estimated.
Results: Out of 32 tubercular patients, 28 had CSF-ADA at or
above the cut-off value while four had below. Out of 24 nontuberculous patients (pyogenic and aseptic meningitis), two
aseptic meningitis (AM) patient had ADA levels at or above
the cut-off value while 22 had below this value. Results of our
study indicate that ADA level estimation in CSF is not only of
considerable value in the diagnosis of TBM, CSF, and ADA level
10 U/L as a cut-off value with sensitivity 87.5% and specificity
83.33% and positive predictive value of the test was 87.5%.and
83.3% negative predictive value.
Conclusion: It can be concluded that ADA estimation in CSF
is not only simple, inexpensive and rapid but also fairly specific
method for making a diagnosis of tuberculous aetiology in
TBM, especially when there is a dilemma of differentiating the
tuberculous aetiology from non-tuberculous ones. For this
reason ADA estimation in TBM may find a place as a routine
investigation. |
| first_indexed | 2024-12-11T06:11:30Z |
| format | Article |
| id | doaj.art-36a80526e7b4454383c4e11e32262047 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-11T06:11:30Z |
| publishDate | 2014-02-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-36a80526e7b4454383c4e11e322620472022-12-22T01:18:07ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-02-0182737610.7860/JCDR/2014/7154.4012A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of MeningitisAshok Kumar Agarwal0Sonia Bansal1Vidya Nand2Senior Resident, Department of Medicine, Rajendra Institute of Medical Science (RIMS), Ranchi, Jharkhand, India.Microbiologist (Ex-Junior Resident), Department of Microbiology, Rajendra Institute of Medical Science (RIMS), Ranchi, Jharkhand, India.Assistant Professor, Department of Medicine, Rohilkhand Medical College, Bareilly, Uttar Pradesh, India.Objective: Tuberculosis kills 3.70 lakh patients in India every year,out of which 7-12 % are meningeal involvement. Delay in its diagnosis and initiation of treatment results in poor prognosis and squeal in up to 25% of cases. The aim of the present study is to look for a simple, rapid, cost effective, and fairly specific test in differentiating tubercular aetiology from other causes of meningitis. In the present study we measured the adenosine deaminase activity (ADA) in Cerebrospinal Fluid (CSF) of Tubercular Meningitis (TBM) and non-TBM patients. Methods: Fifty six patients attending hospital with symptoms and signs of meningitis were selected and divided into three groups: tubercular, pyogenic, and aseptic meningitis, depending upon the accepted criteria. CSF was drawn and ADA estimated. Results: Out of 32 tubercular patients, 28 had CSF-ADA at or above the cut-off value while four had below. Out of 24 nontuberculous patients (pyogenic and aseptic meningitis), two aseptic meningitis (AM) patient had ADA levels at or above the cut-off value while 22 had below this value. Results of our study indicate that ADA level estimation in CSF is not only of considerable value in the diagnosis of TBM, CSF, and ADA level 10 U/L as a cut-off value with sensitivity 87.5% and specificity 83.33% and positive predictive value of the test was 87.5%.and 83.3% negative predictive value. Conclusion: It can be concluded that ADA estimation in CSF is not only simple, inexpensive and rapid but also fairly specific method for making a diagnosis of tuberculous aetiology in TBM, especially when there is a dilemma of differentiating the tuberculous aetiology from non-tuberculous ones. For this reason ADA estimation in TBM may find a place as a routine investigation.https://jcdr.net/articles/PDF/4012/23-%207154_E(Ra)_F(P)_PF1(PAK)_PFA(AK)_PF2(PAK).pdfadenosine deaminase activaity (ada)cerebrospinal fluid (csf)tubercular meningitis (tbm)non-tuberculous meningitis |
| spellingShingle | Ashok Kumar Agarwal Sonia Bansal Vidya Nand A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of Meningitis Journal of Clinical and Diagnostic Research adenosine deaminase activaity (ada) cerebrospinal fluid (csf) tubercular meningitis (tbm) non-tuberculous meningitis |
| title | A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of Meningitis |
| title_full | A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of Meningitis |
| title_fullStr | A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of Meningitis |
| title_full_unstemmed | A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of Meningitis |
| title_short | A Hospital Based Study on Estimation of Adenosine Deaminase Activity (ADA) in Cerebrospinal Fluid (CSF) in Various Types of Meningitis |
| title_sort | hospital based study on estimation of adenosine deaminase activity ada in cerebrospinal fluid csf in various types of meningitis |
| topic | adenosine deaminase activaity (ada) cerebrospinal fluid (csf) tubercular meningitis (tbm) non-tuberculous meningitis |
| url | https://jcdr.net/articles/PDF/4012/23-%207154_E(Ra)_F(P)_PF1(PAK)_PFA(AK)_PF2(PAK).pdf |
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