Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries
Human low-molecular weight salivary mucin (MUC7) is a small, secreted glycoprotein coded by MUC7. In the oral cavity, they inhibit the colonization of oral bacteria, including cariogenic ones, by masking their surface adhesions, thus helping saliva to avoid dental caries. The N-terminal domain is im...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2016-06-01
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| Series: | Balkan Journal of Medical Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/bjmg-2016-0009 |
| _version_ | 1797705309852008448 |
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| author | Koç Öztürk L Yarat A Akyuz S Furuncuoglu H Ulucan K |
| author_facet | Koç Öztürk L Yarat A Akyuz S Furuncuoglu H Ulucan K |
| author_sort | Koç Öztürk L |
| collection | DOAJ |
| description | Human low-molecular weight salivary mucin (MUC7) is a small, secreted glycoprotein coded by MUC7. In the oral cavity, they inhibit the colonization of oral bacteria, including cariogenic ones, by masking their surface adhesions, thus helping saliva to avoid dental caries. The N-terminal domain is important for low-molecular weight (MG2) mucins to contact with oral microorganisms. In this study, we aimed to identify the N-terminal coding region of the MUC7 gene between individuals with and without caries. Forty-four healthy dental students were enrolled in this study; 24 of them were classified to have caries [decayed, missing, filled-teeth (DMFT) = 5.6] according to the World Health Organization (WHO) criteria, and 20 of them were caries-free (DMFT = 0). Simplified oral hygiene index (OHI-S) and gingival index (GI) were used to determine the oral hygiene and gingival conditions. Total protein levels and salivary total protein levels and salivary buffer capacity (SBC) were determined by Lowry and Ericsson methods. DNA was extracted from peripheral blood cells of all the participants and genotyping was carried out by a polymerase chain reaction (PCR)-sequencing method. No statistical differences were found between two groups in the terms of salivary parameters, oral hygiene and gingival conditions. We detected one common single nucleotide polymorphism (SNP) that leads to a change of asparagine to lysine at codon 80. This substitution was found in 29.0 and 40.0%, respectively, of the groups with and without caries. No other sequence variations were detected. The SNP found in this study may be a specific polymorphism affecting the Turkish population. Further studies with extended numbers are necessary in order to clarify this finding. |
| first_indexed | 2024-03-12T05:34:14Z |
| format | Article |
| id | doaj.art-36b3072aa9cd44528e94c9e3b76472f3 |
| institution | Directory Open Access Journal |
| issn | 1311-0160 |
| language | English |
| last_indexed | 2024-03-12T05:34:14Z |
| publishDate | 2016-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Balkan Journal of Medical Genetics |
| spelling | doaj.art-36b3072aa9cd44528e94c9e3b76472f32023-09-03T06:32:28ZengSciendoBalkan Journal of Medical Genetics1311-01602016-06-01191717610.1515/bjmg-2016-0009bjmg-2016-0009Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without cariesKoç Öztürk L0Yarat A1Akyuz S2Furuncuoglu H3Ulucan K4Department of Basic Medical Sciences, Faculty of Dentistry, Marmara University, Istanbul, TurkeyDepartment of Basic Medical Sciences, Faculty of Dentistry, Marmara University, Istanbul, Turkey Department of Clinical Sciences, Faculty of Dentristry, Marmara University, Istanbul, Turkey Department of Clinical Sciences, Faculty of Dentristry, Marmara University, Istanbul, TurkeyDepartment of Basic Medical Sciences, Faculty of Dentistry, Marmara University, Istanbul, TurkeyHuman low-molecular weight salivary mucin (MUC7) is a small, secreted glycoprotein coded by MUC7. In the oral cavity, they inhibit the colonization of oral bacteria, including cariogenic ones, by masking their surface adhesions, thus helping saliva to avoid dental caries. The N-terminal domain is important for low-molecular weight (MG2) mucins to contact with oral microorganisms. In this study, we aimed to identify the N-terminal coding region of the MUC7 gene between individuals with and without caries. Forty-four healthy dental students were enrolled in this study; 24 of them were classified to have caries [decayed, missing, filled-teeth (DMFT) = 5.6] according to the World Health Organization (WHO) criteria, and 20 of them were caries-free (DMFT = 0). Simplified oral hygiene index (OHI-S) and gingival index (GI) were used to determine the oral hygiene and gingival conditions. Total protein levels and salivary total protein levels and salivary buffer capacity (SBC) were determined by Lowry and Ericsson methods. DNA was extracted from peripheral blood cells of all the participants and genotyping was carried out by a polymerase chain reaction (PCR)-sequencing method. No statistical differences were found between two groups in the terms of salivary parameters, oral hygiene and gingival conditions. We detected one common single nucleotide polymorphism (SNP) that leads to a change of asparagine to lysine at codon 80. This substitution was found in 29.0 and 40.0%, respectively, of the groups with and without caries. No other sequence variations were detected. The SNP found in this study may be a specific polymorphism affecting the Turkish population. Further studies with extended numbers are necessary in order to clarify this finding.https://doi.org/10.1515/bjmg-2016-0009dental cariesoral hygine indicislow molecular weight salivar mucin (muc7) |
| spellingShingle | Koç Öztürk L Yarat A Akyuz S Furuncuoglu H Ulucan K Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries Balkan Journal of Medical Genetics dental caries oral hygine indicis low molecular weight salivar mucin (muc7) |
| title | Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries |
| title_full | Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries |
| title_fullStr | Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries |
| title_full_unstemmed | Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries |
| title_short | Investigation of the N-terminal coding region of MUC7 alterations in dentistry students with and without caries |
| title_sort | investigation of the n terminal coding region of muc7 alterations in dentistry students with and without caries |
| topic | dental caries oral hygine indicis low molecular weight salivar mucin (muc7) |
| url | https://doi.org/10.1515/bjmg-2016-0009 |
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