Role of mitochondria transmembrane potential in lymphocyte apoptosis
Background: Mitochondria play an important role in the regulation of physiological type of cell death (Apoptosis) this type of cell death can be stimulated by two major pathways: external (Fas-Fas ligand interaction) and internal mitochondrial pathway which require disruption of mitochondrial transm...
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| Format: | Article |
| Language: | English |
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College of Medicine University of Baghdad
2009-04-01
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| Series: | مجلة كلية الطب |
| Subjects: | |
| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1180 |
| _version_ | 1797368643460268032 |
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| author | Israa F. Jaffar |
| author_facet | Israa F. Jaffar |
| author_sort | Israa F. Jaffar |
| collection | DOAJ |
| description | Background: Mitochondria play an important role in the regulation of physiological type of cell death (Apoptosis) this type of cell death can be stimulated by two major pathways: external (Fas-Fas ligand interaction) and internal mitochondrial pathway which require disruption of mitochondrial transmembrane potential (ΔΨm ) which leads to opening of mitochondrial channels that lead to release of cytochrome –C which would execute apoptotic process.
Patients and Methods:The study included 32 normal subjects; 2 ml of venous blood were aspirated from each of them and processed for peripheral blood lymphocytes separation (PBL)
Then lymphocyte apoptosis was studied before and after exposing (PBL) to hyperthermia by these three methods to compare which of them can detect earliest apoptotic changes: Cell count and viability, morphological changes (by acredine orange stain) and the changes in mitochondrial membrane potential by Mitolight Apoptosis Detection Kit.
Results: Our results showed that the percentage of lymphocyte apoptosis detected by mitolight was significantly higher than those detected by other two methods.
Conclusion: This study shows that mitochondrial changes can detect earlier apoptotic process than morphological features detected by DNA stains. |
| first_indexed | 2024-03-08T17:35:42Z |
| format | Article |
| id | doaj.art-36b8a88561584fc99fa4aed79d06f2d3 |
| institution | Directory Open Access Journal |
| issn | 0041-9419 2410-8057 |
| language | English |
| last_indexed | 2024-03-08T17:35:42Z |
| publishDate | 2009-04-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj.art-36b8a88561584fc99fa4aed79d06f2d32024-01-02T12:34:41ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572009-04-0151110.32007/1180%g71-75Role of mitochondria transmembrane potential in lymphocyte apoptosisIsraa F. Jaffar0Department of Physiology- Medical College- Baghdad UniversityBackground: Mitochondria play an important role in the regulation of physiological type of cell death (Apoptosis) this type of cell death can be stimulated by two major pathways: external (Fas-Fas ligand interaction) and internal mitochondrial pathway which require disruption of mitochondrial transmembrane potential (ΔΨm ) which leads to opening of mitochondrial channels that lead to release of cytochrome –C which would execute apoptotic process. Patients and Methods:The study included 32 normal subjects; 2 ml of venous blood were aspirated from each of them and processed for peripheral blood lymphocytes separation (PBL) Then lymphocyte apoptosis was studied before and after exposing (PBL) to hyperthermia by these three methods to compare which of them can detect earliest apoptotic changes: Cell count and viability, morphological changes (by acredine orange stain) and the changes in mitochondrial membrane potential by Mitolight Apoptosis Detection Kit. Results: Our results showed that the percentage of lymphocyte apoptosis detected by mitolight was significantly higher than those detected by other two methods. Conclusion: This study shows that mitochondrial changes can detect earlier apoptotic process than morphological features detected by DNA stains.http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1180ΔΨm mitochondrial transmembrane potential. Mitolight, Lymphocyte apoptosis. |
| spellingShingle | Israa F. Jaffar Role of mitochondria transmembrane potential in lymphocyte apoptosis مجلة كلية الطب ΔΨm mitochondrial transmembrane potential. Mitolight, Lymphocyte apoptosis. |
| title | Role of mitochondria transmembrane potential in lymphocyte apoptosis |
| title_full | Role of mitochondria transmembrane potential in lymphocyte apoptosis |
| title_fullStr | Role of mitochondria transmembrane potential in lymphocyte apoptosis |
| title_full_unstemmed | Role of mitochondria transmembrane potential in lymphocyte apoptosis |
| title_short | Role of mitochondria transmembrane potential in lymphocyte apoptosis |
| title_sort | role of mitochondria transmembrane potential in lymphocyte apoptosis |
| topic | ΔΨm mitochondrial transmembrane potential. Mitolight, Lymphocyte apoptosis. |
| url | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1180 |
| work_keys_str_mv | AT israafjaffar roleofmitochondriatransmembranepotentialinlymphocyteapoptosis |