Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.)
Abstract Background In hexaploid wheat, quantitative trait loci (QTL) and meta-QTL (MQTL) analyses were conducted to identify genomic regions controlling resistance to cereal cyst nematode (CCN), Heterodera avenae. A mapping population comprising 149 RILs derived from the cross HUW 468 × C 306 was u...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04526-y |
| _version_ | 1797637030725812224 |
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| author | Saksham Pundir Rakhi Singh Vikas Kumar Singh Shiveta Sharma Harindra Singh Balyan Pushpendra Kumar Gupta Shailendra Sharma |
| author_facet | Saksham Pundir Rakhi Singh Vikas Kumar Singh Shiveta Sharma Harindra Singh Balyan Pushpendra Kumar Gupta Shailendra Sharma |
| author_sort | Saksham Pundir |
| collection | DOAJ |
| description | Abstract Background In hexaploid wheat, quantitative trait loci (QTL) and meta-QTL (MQTL) analyses were conducted to identify genomic regions controlling resistance to cereal cyst nematode (CCN), Heterodera avenae. A mapping population comprising 149 RILs derived from the cross HUW 468 × C 306 was used for composite interval mapping (CIM) and inclusive composite interval mapping (ICIM). Results Eight main effect QTLs on three chromosomes (1B, 2A and 3A) were identified using two repeat experiments. One of these QTLs was co-localized with a previously reported wheat gene Cre5 for resistance to CCN. Seven important digenic epistatic interactions (PVE = 5% or more) were also identified, each involving one main effect QTL and another novel E-QTL. Using QTLs earlier reported in literature, two meta-QTLs were also identified, which were also used for identification of 57 candidate genes (CGs). Out of these, 29 CGs have high expression in roots and encoded the following proteins having a role in resistance to plant parasitic nematodes (PPNs): (i) NB-ARC,P-loop containing NTP hydrolase, (ii) Protein Kinase, (iii) serine-threonine/tyrosine-PK, (iv) protein with leucine-rich repeat, (v) virus X resistance protein-like, (vi) zinc finger protein, (vii) RING/FYVE/PHD-type, (viii) glycosyl transferase, family 8 (GT8), (ix) rubisco protein with small subunit domain, (x) protein with SANT/Myb domain and (xi) a protein with a homeobox. Conclusion Identification and selection of resistance loci with additive and epistatic effect along with two MQTL and associated CGs, identified in the present study may prove useful for understanding the molecular basis of resistance against H. avenae in wheat and for marker-assisted selection (MAS) for breeding CCN resistant wheat cultivars. |
| first_indexed | 2024-03-11T12:42:31Z |
| format | Article |
| id | doaj.art-36b8a8babbd546599f0e1e3e1cb53836 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-11T12:42:31Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-36b8a8babbd546599f0e1e3e1cb538362023-11-05T12:11:51ZengBMCBMC Plant Biology1471-22292023-10-0123111410.1186/s12870-023-04526-yMapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.)Saksham Pundir0Rakhi Singh1Vikas Kumar Singh2Shiveta Sharma3Harindra Singh Balyan4Pushpendra Kumar Gupta5Shailendra Sharma6Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU)Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU)Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU)Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU)Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU)Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU)Department of Genetics and Plant Breeding, Chaudhary Charan Singh University (CCSU)Abstract Background In hexaploid wheat, quantitative trait loci (QTL) and meta-QTL (MQTL) analyses were conducted to identify genomic regions controlling resistance to cereal cyst nematode (CCN), Heterodera avenae. A mapping population comprising 149 RILs derived from the cross HUW 468 × C 306 was used for composite interval mapping (CIM) and inclusive composite interval mapping (ICIM). Results Eight main effect QTLs on three chromosomes (1B, 2A and 3A) were identified using two repeat experiments. One of these QTLs was co-localized with a previously reported wheat gene Cre5 for resistance to CCN. Seven important digenic epistatic interactions (PVE = 5% or more) were also identified, each involving one main effect QTL and another novel E-QTL. Using QTLs earlier reported in literature, two meta-QTLs were also identified, which were also used for identification of 57 candidate genes (CGs). Out of these, 29 CGs have high expression in roots and encoded the following proteins having a role in resistance to plant parasitic nematodes (PPNs): (i) NB-ARC,P-loop containing NTP hydrolase, (ii) Protein Kinase, (iii) serine-threonine/tyrosine-PK, (iv) protein with leucine-rich repeat, (v) virus X resistance protein-like, (vi) zinc finger protein, (vii) RING/FYVE/PHD-type, (viii) glycosyl transferase, family 8 (GT8), (ix) rubisco protein with small subunit domain, (x) protein with SANT/Myb domain and (xi) a protein with a homeobox. Conclusion Identification and selection of resistance loci with additive and epistatic effect along with two MQTL and associated CGs, identified in the present study may prove useful for understanding the molecular basis of resistance against H. avenae in wheat and for marker-assisted selection (MAS) for breeding CCN resistant wheat cultivars.https://doi.org/10.1186/s12870-023-04526-yWheatCereal cyst nematode (CCN)Heterodera avenaeMain effect QTLsEpistatic interactionsmeta-QTLs and candidate genes |
| spellingShingle | Saksham Pundir Rakhi Singh Vikas Kumar Singh Shiveta Sharma Harindra Singh Balyan Pushpendra Kumar Gupta Shailendra Sharma Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.) BMC Plant Biology Wheat Cereal cyst nematode (CCN) Heterodera avenae Main effect QTLs Epistatic interactions meta-QTLs and candidate genes |
| title | Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.) |
| title_full | Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.) |
| title_fullStr | Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.) |
| title_full_unstemmed | Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.) |
| title_short | Mapping of QTLs and meta-QTLs for Heterodera avenae Woll. resistance in common wheat (Triticum aestivum L.) |
| title_sort | mapping of qtls and meta qtls for heterodera avenae woll resistance in common wheat triticum aestivum l |
| topic | Wheat Cereal cyst nematode (CCN) Heterodera avenae Main effect QTLs Epistatic interactions meta-QTLs and candidate genes |
| url | https://doi.org/10.1186/s12870-023-04526-y |
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