The Genomic Basis of Postponed Senescence in Drosophila melanogaster.
Natural populations harbor considerable genetic variation for lifespan. While evolutionary theory provides general explanations for the existence of this variation, our knowledge of the genes harboring naturally occurring polymorphisms affecting lifespan is limited. Here, we assessed the genetic div...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4574564?pdf=render |
| _version_ | 1818193397235580928 |
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| author | Megan Ulmer Carnes Terry Campbell Wen Huang Daniel G Butler Mary Anna Carbone Laura H Duncan Sasha V Harbajan Edward M King Kara R Peterson Alexander Weitzel Shanshan Zhou Trudy F C Mackay |
| author_facet | Megan Ulmer Carnes Terry Campbell Wen Huang Daniel G Butler Mary Anna Carbone Laura H Duncan Sasha V Harbajan Edward M King Kara R Peterson Alexander Weitzel Shanshan Zhou Trudy F C Mackay |
| author_sort | Megan Ulmer Carnes |
| collection | DOAJ |
| description | Natural populations harbor considerable genetic variation for lifespan. While evolutionary theory provides general explanations for the existence of this variation, our knowledge of the genes harboring naturally occurring polymorphisms affecting lifespan is limited. Here, we assessed the genetic divergence between five Drosophila melanogaster lines selected for postponed senescence for over 170 generations (O lines) and five lines from the same base population maintained at a two week generation interval for over 850 generations (B lines). On average, O lines live 70% longer than B lines, are more productive at all ages, and have delayed senescence for other traits than reproduction. We performed population sequencing of pools of individuals from all B and O lines and identified 6,394 genetically divergent variants in or near 1,928 genes at a false discovery rate of 0.068. A 2.6 Mb region at the tip of the X chromosome contained many variants fixed for alternative alleles in the two populations, suggestive of a hard selective sweep. We also assessed genome wide gene expression of O and B lines at one and five weeks of age using RNA sequencing and identified genes with significant (false discovery rate < 0.05) effects on gene expression with age, population and the age by population interaction, separately for each sex. We identified transcripts that exhibited the transcriptional signature of postponed senescence and integrated the gene expression and genetic divergence data to identify 98 (175) top candidate genes in females (males) affecting postponed senescence and increased lifespan. While several of these genes have been previously associated with Drosophila lifespan, most are novel and constitute a rich resource for future functional validation. |
| first_indexed | 2024-12-12T00:45:45Z |
| format | Article |
| id | doaj.art-36bb4da953e449adb4ef2286161087cd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T00:45:45Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-36bb4da953e449adb4ef2286161087cd2022-12-22T00:44:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013856910.1371/journal.pone.0138569The Genomic Basis of Postponed Senescence in Drosophila melanogaster.Megan Ulmer CarnesTerry CampbellWen HuangDaniel G ButlerMary Anna CarboneLaura H DuncanSasha V HarbajanEdward M KingKara R PetersonAlexander WeitzelShanshan ZhouTrudy F C MackayNatural populations harbor considerable genetic variation for lifespan. While evolutionary theory provides general explanations for the existence of this variation, our knowledge of the genes harboring naturally occurring polymorphisms affecting lifespan is limited. Here, we assessed the genetic divergence between five Drosophila melanogaster lines selected for postponed senescence for over 170 generations (O lines) and five lines from the same base population maintained at a two week generation interval for over 850 generations (B lines). On average, O lines live 70% longer than B lines, are more productive at all ages, and have delayed senescence for other traits than reproduction. We performed population sequencing of pools of individuals from all B and O lines and identified 6,394 genetically divergent variants in or near 1,928 genes at a false discovery rate of 0.068. A 2.6 Mb region at the tip of the X chromosome contained many variants fixed for alternative alleles in the two populations, suggestive of a hard selective sweep. We also assessed genome wide gene expression of O and B lines at one and five weeks of age using RNA sequencing and identified genes with significant (false discovery rate < 0.05) effects on gene expression with age, population and the age by population interaction, separately for each sex. We identified transcripts that exhibited the transcriptional signature of postponed senescence and integrated the gene expression and genetic divergence data to identify 98 (175) top candidate genes in females (males) affecting postponed senescence and increased lifespan. While several of these genes have been previously associated with Drosophila lifespan, most are novel and constitute a rich resource for future functional validation.http://europepmc.org/articles/PMC4574564?pdf=render |
| spellingShingle | Megan Ulmer Carnes Terry Campbell Wen Huang Daniel G Butler Mary Anna Carbone Laura H Duncan Sasha V Harbajan Edward M King Kara R Peterson Alexander Weitzel Shanshan Zhou Trudy F C Mackay The Genomic Basis of Postponed Senescence in Drosophila melanogaster. PLoS ONE |
| title | The Genomic Basis of Postponed Senescence in Drosophila melanogaster. |
| title_full | The Genomic Basis of Postponed Senescence in Drosophila melanogaster. |
| title_fullStr | The Genomic Basis of Postponed Senescence in Drosophila melanogaster. |
| title_full_unstemmed | The Genomic Basis of Postponed Senescence in Drosophila melanogaster. |
| title_short | The Genomic Basis of Postponed Senescence in Drosophila melanogaster. |
| title_sort | genomic basis of postponed senescence in drosophila melanogaster |
| url | http://europepmc.org/articles/PMC4574564?pdf=render |
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