Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2
Hepatitis C Virus (HCV) encodes two envelope glycoproteins, E1 and E2. Our previous work selected a specific aptamer ZE2, which could bind to E2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting HCV. In this...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2015-01-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/index.php/IJMS/article/view/1392 |
| _version_ | 1818580261521063936 |
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| author | Fan Chen Si-Chong Chen Jing Zhou Zhi-De Chen Fang Chen |
| author_facet | Fan Chen Si-Chong Chen Jing Zhou Zhi-De Chen Fang Chen |
| author_sort | Fan Chen |
| collection | DOAJ |
| description | Hepatitis C Virus (HCV) encodes two envelope glycoproteins, E1 and E2. Our previous work selected a specific aptamer ZE2, which could bind to E2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting HCV. In this study, the binding sites between E2 and aptamer ZE2 were further explored. E2 was truncated to 15 peptides (P1 to P15) and these peptides were used to detect the affinity with ZE2 by ELISA respectively. The peptide with high affinity was then further truncated, detected and compared with six kinds of HCV genotypes. The basic amino acid in 500 aa bound to ZE2 with high affinity, while acidic amino acid in 501 aa reduced the reaction between E2 and ZE2. The results showed the 500 aa and 501 aa of E2 were the key sites that bound to ZE2. |
| first_indexed | 2024-12-16T07:14:47Z |
| format | Article |
| id | doaj.art-36bdc2c2ecbc482dbf85189bc995919f |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-12-16T07:14:47Z |
| publishDate | 2015-01-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-36bdc2c2ecbc482dbf85189bc995919f2022-12-21T22:39:48ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882015-01-014016367Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2Fan Chen0Si-Chong Chen1Jing Zhou2Zhi-De Chen3Fang Chen4Department of Biochemistry and Molecular Biology, Life Sciences School of Hubei University, Wuhan, China;Evolution and Ecology Research Centre, School of Biological, Earth and Environmental Sciences, The University of New South Wales, Sydney, Australia;Clinical laboratory, Wuhan Tuberculosis Dispensary, Wuhan Health Bureau, Wuhan, China;Department of Biochemistry and Molecular Biology, Life Sciences School of Hubei University, Wuhan, China;Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Wuhan University, Wuhan, ChinaHepatitis C Virus (HCV) encodes two envelope glycoproteins, E1 and E2. Our previous work selected a specific aptamer ZE2, which could bind to E2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting HCV. In this study, the binding sites between E2 and aptamer ZE2 were further explored. E2 was truncated to 15 peptides (P1 to P15) and these peptides were used to detect the affinity with ZE2 by ELISA respectively. The peptide with high affinity was then further truncated, detected and compared with six kinds of HCV genotypes. The basic amino acid in 500 aa bound to ZE2 with high affinity, while acidic amino acid in 501 aa reduced the reaction between E2 and ZE2. The results showed the 500 aa and 501 aa of E2 were the key sites that bound to ZE2.http://ijms.sums.ac.ir/index.php/IJMS/article/view/1392Hepatitis C virusGlycoprotein E2AptamerBinding site |
| spellingShingle | Fan Chen Si-Chong Chen Jing Zhou Zhi-De Chen Fang Chen Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2 Iranian Journal of Medical Sciences Hepatitis C virus Glycoprotein E2 Aptamer Binding site |
| title | Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2 |
| title_full | Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2 |
| title_fullStr | Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2 |
| title_full_unstemmed | Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2 |
| title_short | Identification of Aptamer-Binding Sites in Hepatitis C Virus Envelope Glycoprotein E2 |
| title_sort | identification of aptamer binding sites in hepatitis c virus envelope glycoprotein e2 |
| topic | Hepatitis C virus Glycoprotein E2 Aptamer Binding site |
| url | http://ijms.sums.ac.ir/index.php/IJMS/article/view/1392 |
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