Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeria
The present study was carried out to investigate the presence of Clostridium perfringens (C. perfringens) in broiler chickens from various locations in Tiaret province, western Algeria, and to characterize the bacterium isolates for the presence of cpa, cpb, etx, iA and netB gene. A total of 180 sam...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2017-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2089 |
| _version_ | 1797800557390331904 |
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| author | MERATI R TEMIM S MOHAMED AAAF |
| author_facet | MERATI R TEMIM S MOHAMED AAAF |
| author_sort | MERATI R |
| collection | DOAJ |
| description | The present study was carried out to investigate the presence of Clostridium perfringens (C. perfringens) in broiler chickens from various
locations in Tiaret province, western Algeria, and to characterize the bacterium isolates for the presence of cpa, cpb, etx, iA and netB gene.
A total of 180 samples representing intestinal contents of broiler chickens showing enteric disorder symptoms and lesions suspected to
be Necrotic Enteritis (NE) were analyzed by conventional methods and polymerase chain reaction (PCR). C. perfringens was isolated at the
rate of 34.44% (62/180), and its presence was conrmed by cultural and biochemical characterization. 83.87% (52/62) C. perfringens isolates
were toxigenic and 16.13% (10/62) were non-toxigenic. Multiplex PCR was performed to toxinotype the 52 toxigenic isolates, and the results
showed that all isolates were positive for the gene cpa and negative for cpb, etx and iA. This indicates that all the toxigenic isolates were C.
perfringens type A (52/52). Uniplex PCR for detection of NetB toxin gene was carried out on 22 type A isolates, and these results showed
none of the isolates as positive for the gene netB. This result indicates that the C. perfringens type A was the most predominant etiology of
NE without carrying the netB gene. |
| first_indexed | 2024-03-13T04:37:08Z |
| format | Article |
| id | doaj.art-36bdf4e1dc6146fab9a81b5a015af9e7 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:08Z |
| publishDate | 2017-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-36bdf4e1dc6146fab9a81b5a015af9e72023-06-19T06:45:26ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-07-0123459560110.9775/kvfd.2017.174312089Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeriaMERATI R0TEMIM S1MOHAMED AAAF2Laboratoire d’Hygiène et Pathologie Animale, Université Ibn Khaldoun, 14000, Tiaret, ALGERIELaboratoire de Recherche « Santé et Production Animales », Ecole Nationale Supérieure Vétérinaire, 16000, Alger, ALGERIEVeterinary Serum and Vaccine Research Institute, 11765, Abassia, Cairo, EGYPTThe present study was carried out to investigate the presence of Clostridium perfringens (C. perfringens) in broiler chickens from various locations in Tiaret province, western Algeria, and to characterize the bacterium isolates for the presence of cpa, cpb, etx, iA and netB gene. A total of 180 samples representing intestinal contents of broiler chickens showing enteric disorder symptoms and lesions suspected to be Necrotic Enteritis (NE) were analyzed by conventional methods and polymerase chain reaction (PCR). C. perfringens was isolated at the rate of 34.44% (62/180), and its presence was conrmed by cultural and biochemical characterization. 83.87% (52/62) C. perfringens isolates were toxigenic and 16.13% (10/62) were non-toxigenic. Multiplex PCR was performed to toxinotype the 52 toxigenic isolates, and the results showed that all isolates were positive for the gene cpa and negative for cpb, etx and iA. This indicates that all the toxigenic isolates were C. perfringens type A (52/52). Uniplex PCR for detection of NetB toxin gene was carried out on 22 type A isolates, and these results showed none of the isolates as positive for the gene netB. This result indicates that the C. perfringens type A was the most predominant etiology of NE without carrying the netB gene.https://vetdergikafkas.org/pdf.php?id=2089clostridium perfringensnecrotic enteritisbroilertoxinotypingnetb |
| spellingShingle | MERATI R TEMIM S MOHAMED AAAF Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeria Kafkas Universitesi Veteriner Fakültesi Dergisi clostridium perfringens necrotic enteritis broiler toxinotyping netb |
| title | Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeria |
| title_full | Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeria |
| title_fullStr | Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeria |
| title_full_unstemmed | Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeria |
| title_short | Identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret, western algeria |
| title_sort | identification and characterization of clostridium perfringens isolated from necrotic enteritis in broiler chickens in tiaret western algeria |
| topic | clostridium perfringens necrotic enteritis broiler toxinotyping netb |
| url | https://vetdergikafkas.org/pdf.php?id=2089 |
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