| Summary: | The production of cucumber (<i>Cucumis sativus</i> L.) is often harmed by biotic and abiotic stresses. Although the dehydration-responsive element-binding (DREB) transcription factors, playing vital roles in stress responses, have been characterized in several plant species, little is known about the pan-genome characteristics of <i>DREB</i> genes and their expression patterns under different stresses in cucumber. In this study, we identified 55 CsDREBs from the cucumber pan-genomes of 13 accessions, but only four accessions had all the genes. Most of the CsDREB proteins had sequence length and/or amino acid variations, and only four of them had no variation among different accessions. Using the 55 <i>CsDREBs</i> from ‘9930’, we analyzed their gene structures, conserved domains, phylogenetic relationships, gene promoter’s cis-elements and syntenic relationships, and classified them into six groups. Expression pattern analysis revealed that eight <i>CsDREBs</i> showed constitutive expression (FPKM > 1 in all samples), and different <i>CsDREBs</i> showed specifically high expression in root, stem, leaf, tendril, male-flower, female flower, and ovary, respectively, suggesting that these genes might be important for morphogenesis and development in cucumber. Additionally, a total of 31, 22, 30 and nine <i>CsDREBs</i> were differentially expressed in responding to the treatments of heat, NaCl and/or silicon, power mildew and downy mildew, respectively. Interestingly, <i>CsDREB33</i> could respond to all the tested stresses. Our results provide a reference and basis for further investigation of the function and mechanism of the <i>DREB</i> genes for resistance breeding in cucumber.
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