VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients
Introduction: The Vascular Adhesion Molecule-1 (VCAM-1) is a cell surface glycoprotein expressed by cytokine-activated endothelium, mediates the adhesion of monocytes and lymphocytes. Diabetes mellitus is a common global health problem represented by metabolic disorder in which common genetic v...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/10932/29397_CE(RA1)_F(T)_PF1(AG_TG_AP)_PFA(MJ_AP).pdf |
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| author | Nadia Fadhil Salman Asmaa M Salih Almohaidi Ahmed Kadhim Mohammed Dhurgham Hameed Hasan |
| author_facet | Nadia Fadhil Salman Asmaa M Salih Almohaidi Ahmed Kadhim Mohammed Dhurgham Hameed Hasan |
| author_sort | Nadia Fadhil Salman |
| collection | DOAJ |
| description | Introduction: The Vascular Adhesion Molecule-1 (VCAM-1) is
a cell surface glycoprotein expressed by cytokine-activated
endothelium, mediates the adhesion of monocytes and
lymphocytes. Diabetes mellitus is a common global health
problem represented by metabolic disorder in which common
genetic variants interact with environmental factors like older
age, obesity, family history of diabetes to unmask the disease
which might be a result of inflammation reflecting response.
Aim: Detection of the VCAM-1(rs3783605 A>G) gene polymorphism in a group of Type 2 Diabetes Mellitus (T2DM) Iraqi
patients.
Materials and Methods: The study enrolled 129 subjects
(68 patients, 61 controls). Clinical features and laboratory
investigations included: Body Mass Index, Abdominal
Circumference, fasting blood sugar, Glycohemoglobin (HbA1c)
and lipid profile were measured for each subject. Genotyping
of the VCAM-1(rs3783605 A>G) gene polymorphism was
performed using Sequence Specific Primer (SSP) Polymerase
Chain Reaction (PCR) technique and the PCR product was
visualized by agarose gel electrophoresis.
Results: The clinical characteristics showed a significant
increase (p<0.05) in the patients group compared to the
control group. VCAM-1polymorphism analysis showed a higher
frequency of A/G genotype in control group than patients group
(100% versus 88.2%), while the analysis of the allele impact,
including the genotypes that contain the G allele showed no
significant association in patient group compared with control.
Conclusion: The results of the present study showed that
control group had a higher frequency of the A/G genotype
regarding the patients group, and that the G allele of the VCAM-1
(rs3783605 A>G) single nucleotide polymorphism has more role
in the pathogenesis of T2DM than the A allele, and could be
considered as a risk factor for T2DM in Iraqi population, as well
as the association of the A/G genotype with the incidence of
T2DM in Iraqi patients. |
| first_indexed | 2024-12-21T10:29:08Z |
| format | Article |
| id | doaj.art-36c42a7e877b466a95e2de46114dc079 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T10:29:08Z |
| publishDate | 2017-12-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-36c42a7e877b466a95e2de46114dc0792022-12-21T19:07:14ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-12-011112GC01GC0510.7860/JCDR/2017/29397.10932VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi PatientsNadia Fadhil Salman0Asmaa M Salih Almohaidi1Ahmed Kadhim Mohammed2Dhurgham Hameed Hasan3PhD Student, Department of Biology, Baghdad University, Baghdad, Al Gazalia, Iraq.Assistant Professor, Department of Biology, Baghdad University, Baghdad, Al Gazalia, Iraq.Specialist DNA Analyst, Department of Serology, Medico-Legal Directorate, Baghdad, Iraq.Specialist DNA Analyst, Department of Paternity and Kinship, Medico-Legal Directorate, Baghdad, Iraq.Introduction: The Vascular Adhesion Molecule-1 (VCAM-1) is a cell surface glycoprotein expressed by cytokine-activated endothelium, mediates the adhesion of monocytes and lymphocytes. Diabetes mellitus is a common global health problem represented by metabolic disorder in which common genetic variants interact with environmental factors like older age, obesity, family history of diabetes to unmask the disease which might be a result of inflammation reflecting response. Aim: Detection of the VCAM-1(rs3783605 A>G) gene polymorphism in a group of Type 2 Diabetes Mellitus (T2DM) Iraqi patients. Materials and Methods: The study enrolled 129 subjects (68 patients, 61 controls). Clinical features and laboratory investigations included: Body Mass Index, Abdominal Circumference, fasting blood sugar, Glycohemoglobin (HbA1c) and lipid profile were measured for each subject. Genotyping of the VCAM-1(rs3783605 A>G) gene polymorphism was performed using Sequence Specific Primer (SSP) Polymerase Chain Reaction (PCR) technique and the PCR product was visualized by agarose gel electrophoresis. Results: The clinical characteristics showed a significant increase (p<0.05) in the patients group compared to the control group. VCAM-1polymorphism analysis showed a higher frequency of A/G genotype in control group than patients group (100% versus 88.2%), while the analysis of the allele impact, including the genotypes that contain the G allele showed no significant association in patient group compared with control. Conclusion: The results of the present study showed that control group had a higher frequency of the A/G genotype regarding the patients group, and that the G allele of the VCAM-1 (rs3783605 A>G) single nucleotide polymorphism has more role in the pathogenesis of T2DM than the A allele, and could be considered as a risk factor for T2DM in Iraqi population, as well as the association of the A/G genotype with the incidence of T2DM in Iraqi patients.https://jcdr.net/articles/PDF/10932/29397_CE(RA1)_F(T)_PF1(AG_TG_AP)_PFA(MJ_AP).pdfglycohemoglobintype 2 diabetes mellitusrs3783605gene polymorphism |
| spellingShingle | Nadia Fadhil Salman Asmaa M Salih Almohaidi Ahmed Kadhim Mohammed Dhurgham Hameed Hasan VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients Journal of Clinical and Diagnostic Research glycohemoglobin type 2 diabetes mellitus rs3783605gene polymorphism |
| title | VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients |
| title_full | VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients |
| title_fullStr | VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients |
| title_full_unstemmed | VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients |
| title_short | VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients |
| title_sort | vcam 1 rs3783605a g single nucleotide polymorphism genotyping in a sample of type 2 diabetes mellitus iraqi patients |
| topic | glycohemoglobin type 2 diabetes mellitus rs3783605gene polymorphism |
| url | https://jcdr.net/articles/PDF/10932/29397_CE(RA1)_F(T)_PF1(AG_TG_AP)_PFA(MJ_AP).pdf |
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