β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice
The role of canonical Wnt signaling in metabolic regulation and development of physiological cardiac hypertrophy remains largely unknown. To explore the function of β-catenin in the regulation of cardiac metabolism and physiological cardiac hypertrophy development, we used mice heterozygous for card...
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MDPI AG
2020-12-01
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| Online Access: | https://www.mdpi.com/2075-1729/10/12/357 |
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| author | Volodymyr V. Balatskyi Oksana L. Palchevska Lina Bortnichuk Ana-Maria Gan Anna Myronova Larysa L. Macewicz Viktor O. Navrulin Lesya V. Tumanovska Adam Olichwier Pawel Dobrzyn Oksana O. Piven |
| author_facet | Volodymyr V. Balatskyi Oksana L. Palchevska Lina Bortnichuk Ana-Maria Gan Anna Myronova Larysa L. Macewicz Viktor O. Navrulin Lesya V. Tumanovska Adam Olichwier Pawel Dobrzyn Oksana O. Piven |
| author_sort | Volodymyr V. Balatskyi |
| collection | DOAJ |
| description | The role of canonical Wnt signaling in metabolic regulation and development of physiological cardiac hypertrophy remains largely unknown. To explore the function of β-catenin in the regulation of cardiac metabolism and physiological cardiac hypertrophy development, we used mice heterozygous for cardiac-specific <i>β-catenin</i> knockout that were subjected to a swimming training model. <i>β-Catenin</i> haploinsufficient mice subjected to endurance training displayed a decreased β-catenin transcriptional activity, attenuated cardiomyocytes hypertrophic growth, and enhanced activation of AMP-activated protein kinase (AMPK), phosphoinositide-3-kinase–Akt (Pi3K–Akt), and mitogen-activated protein kinase/extracellular signal-regulated kinases 1/2 (MAPK/Erk1/2) signaling pathways compared to trained wild type mice. We further observed an increased level of proteins involved in glucose aerobic metabolism and β-oxidation along with perturbed activity of mitochondrial oxidative phosphorylation complexes (OXPHOS) in trained <i>β-catenin</i> haploinsufficient mice. Taken together, Wnt/β-catenin signaling appears to govern metabolic regulatory programs, sustaining metabolic plasticity in adult hearts during the adaptation to endurance training. |
| first_indexed | 2024-03-10T13:58:54Z |
| format | Article |
| id | doaj.art-36c8f4c06c47434da057c1e6a569c161 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T13:58:54Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-36c8f4c06c47434da057c1e6a569c1612023-11-21T01:22:23ZengMDPI AGLife2075-17292020-12-01101235710.3390/life10120357β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained MiceVolodymyr V. Balatskyi0Oksana L. Palchevska1Lina Bortnichuk2Ana-Maria Gan3Anna Myronova4Larysa L. Macewicz5Viktor O. Navrulin6Lesya V. Tumanovska7Adam Olichwier8Pawel Dobrzyn9Oksana O. Piven10Department of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Akademika Zabolotnogo Street, 03680 Kyiv, UkraineDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Akademika Zabolotnogo Street, 03680 Kyiv, UkraineDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Akademika Zabolotnogo Street, 03680 Kyiv, UkraineLaboratory of Molecular Medical Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Akademika Zabolotnogo Street, 03680 Kyiv, UkraineDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Akademika Zabolotnogo Street, 03680 Kyiv, UkraineLaboratory of Molecular Medical Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandDepartment of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, 4 Bogomoletz Street, 01024 Kyiv, UkraineLaboratory of Molecular Medical Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandLaboratory of Molecular Medical Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, PolandDepartment of Human Genetics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Akademika Zabolotnogo Street, 03680 Kyiv, UkraineThe role of canonical Wnt signaling in metabolic regulation and development of physiological cardiac hypertrophy remains largely unknown. To explore the function of β-catenin in the regulation of cardiac metabolism and physiological cardiac hypertrophy development, we used mice heterozygous for cardiac-specific <i>β-catenin</i> knockout that were subjected to a swimming training model. <i>β-Catenin</i> haploinsufficient mice subjected to endurance training displayed a decreased β-catenin transcriptional activity, attenuated cardiomyocytes hypertrophic growth, and enhanced activation of AMP-activated protein kinase (AMPK), phosphoinositide-3-kinase–Akt (Pi3K–Akt), and mitogen-activated protein kinase/extracellular signal-regulated kinases 1/2 (MAPK/Erk1/2) signaling pathways compared to trained wild type mice. We further observed an increased level of proteins involved in glucose aerobic metabolism and β-oxidation along with perturbed activity of mitochondrial oxidative phosphorylation complexes (OXPHOS) in trained <i>β-catenin</i> haploinsufficient mice. Taken together, Wnt/β-catenin signaling appears to govern metabolic regulatory programs, sustaining metabolic plasticity in adult hearts during the adaptation to endurance training.https://www.mdpi.com/2075-1729/10/12/357Wnt/β-catenin signalingtraining-induced heart hypertrophyglucose metabolismlipid metabolismβ-oxidationoxidative phosphorylation |
| spellingShingle | Volodymyr V. Balatskyi Oksana L. Palchevska Lina Bortnichuk Ana-Maria Gan Anna Myronova Larysa L. Macewicz Viktor O. Navrulin Lesya V. Tumanovska Adam Olichwier Pawel Dobrzyn Oksana O. Piven β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice Life Wnt/β-catenin signaling training-induced heart hypertrophy glucose metabolism lipid metabolism β-oxidation oxidative phosphorylation |
| title | β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice |
| title_full | β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice |
| title_fullStr | β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice |
| title_full_unstemmed | β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice |
| title_short | β-Catenin Regulates Cardiac Energy Metabolism in Sedentary and Trained Mice |
| title_sort | β catenin regulates cardiac energy metabolism in sedentary and trained mice |
| topic | Wnt/β-catenin signaling training-induced heart hypertrophy glucose metabolism lipid metabolism β-oxidation oxidative phosphorylation |
| url | https://www.mdpi.com/2075-1729/10/12/357 |
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